Superoxide dismutase specific activities in cultured human diploid cells of various donor ages

Duncan, Michael R.; Dell’Orco, Robert T.; Kirk, Kirsty-Anne

doi:10.1002/jcp.1040980219

Cited by 18 publications

(5 citation statements)

References 20 publications

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“…No difference between early and late passage cells was apparent in their data obtained using fetal lung fibroblasts (IMR-90;Linskens et al, 1995). Interestingly, Duncan et al (1979) reported that SOD activity increased with proliferative age in cells derived from fetal skin and lung fibroblasts and newborn foreskin fibroblasts but not in a cell line established from adult skin. Although we occasionally observe an elevation in SOD-2 mRNA abundance and activity in senescent WI-38 cells, we have been unable to investigate this artifact further due to the low frequency of its occurrence.…”

Section: Discussionmentioning

confidence: 88%

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

et al. 1999

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The activities and mRNA abundances of enzymes that regulate the rate of electron flow through the electron transport chain (ETC), including NADH dehydrogenase, succinate dehydrogenase, and cytochrome c oxidase, were examined in young and senescent fetal lung fibroblasts (WI-38). We also determined the activities and mRNA abundances of antioxidant defenses including superoxide dismutase, catalase, and glutathione peroxidase. We confirmed our previous report of a senescence-related increase in the abundance of ND4, a mitochondrially encoded subunit of NADH dehydrogenase. The activities of cytochrome c oxidase and NADH dehydrogenase were also elevated in senescent cultures. No differences were observed in the mRNA abundances of COX-1, a mitochondrially encoded subunit of cytochrome c oxidase or of nuclearly encoded subunits of various electron transport components (SD, COX-4, and ND 51). Lucigenin-detected chemiluminescence and H2O2 generation were both elevated in senescent cells. Catalase activity was also elevated in senescent fibroblasts. However, no differences in catalase mRNA abundance were observed. A small decrease in GSH peroxidase (GPx) mRNA abundance was observed in senescent cells. No other changes in the activities or mRNA abundances of any of the antioxidant defenses were observed in early and late passage cultures. The relationships between oxidant generation, mitochondrial enzyme activities, and antioxidant defense observed during proliferative senescence are dissimilar to those detected between fetal and postnatal fibroblasts as well as those found between fibroblast lines obtained from young and old individuals. The relevance of the differences between these models is discussed.

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Section: Discussionmentioning

confidence: 88%

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

et al. 1999

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“…In addition, reports have indicated increases in SOD‐2 mRNA abundance in senescent fibroblast cultures established from foetal foreskin and adult skin , but not in senescent foetal lung fibroblasts where no changes were observed in both SOD‐1 and SOD‐2 activities and mRNA abundance. In contrast, Duncan et al , reported increased total SOD activity in senescent foetal lung fibroblasts, but not in senescent fibroblasts of adult skin. All the above age‐related controversies in SOD‐1 and SOD‐2 activities can be attributed to the type of fibroblasts studied and whether the cells were derived from donors of different ages or underwent in vitro replicative senescence where the possibility of subjecting the cells to exogenous oxidative stress arises.…”

Section: Discussionmentioning

confidence: 97%

‘The effect of micronutrients on superoxide dismutase in senescent fibroblasts’

Al‐Sheikh

Ghneim

2011

Cell Biochemistry & Function

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The specific activities of zinc/copper (Zn/Cu)-superoxide dismutase (SOD-1) and manganese (Mn)-superoxide dismutase (SOD-2) were assayed in young passage 5 fibroblasts and in serially subcultured cells that were characterized as senescent at passages 15-35. SOD-1 and SOD-2 activities did not significantly change in senescent and young cells cultured in either routine medium [minimum essential medium 1 (MEM1)], or in Zn, Cu and Mn supplemented medium (MEM2) containing normal human plasma levels of the cations. SOD-1 and SOD-2 activities, however, underwent parallel progressive significant activity increases in senescent passage 20 and 25 cells, which peaked in value in passage 30 and 35 cells subcultured in supplemented medium (MEM3) containing triple human plasma levels of the cations. Concurrently, superoxide radical generation rates underwent progressive significant increases in senescent passage 15-25 cells, which peaked in value in passage 30 and 35 cells subcultured in MEM1 or MEM2. These rates, however, were significantly lowered in senescent cells subcultured in MEM3. We infer that it was only possible to significantly stimulate SOD-1 and SOD-2 activities in senescent MEM3 cultured cells enabling them to combat oxidative stress.

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“…There are several reports on aging dependent alterations in the antioxidative capacities of human diploid fibroblasts such as superoxide dismu tase (SOD; EC 1.15.1.1) activity (3)(4)(5), catalase (EC 1.11.1.6) activity (6) and glutathione peroxidase (GSH Px; EC 1.11.1.9) activity (7) or reduced glutathione (GSH) concentration (7). Cytosol contains Cu, Zn-SOD and GSH-Px with three-quarters as much as the total activity within the cell (8).…”

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confidence: 99%

Effect of a Radical Scavenger "Water Soluble Protein" from Broad Beans (Vicia faba) on Antioxidative Enzyme Activity in Cellular Aging.

Okada¹,

Okada

2000

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryWe isolated a free-radical scavenger "water soluble protein (WSP)" from broad beans. Hydrocortisone (HC) is known to inhibit superoxide generation and was used as the reference scavenger. WSP was examined for its effect on antioxidation in young (PDL 20, 25% of the maximum life span) and old (PDL 50, 62.5% of the maximum life span) human fibroblasts (TIG-1). Cells were treated with WSP or HC for 4 and 6wk in young cells, and for 3 and 6 wk in old cells. The cytosolic superoxide dismutase activity in the cells treated with WSP or HC tended to decrease as compared with that in the non-treated cells (control) with the exception of WSP-treated young cells 4 wk after culturing. Young cells were equal in glutathione peroxidase activity to the control, but the activity level in WSP or HC-treated young cells 6 wk after culturing was 10-50% lower than that in the control. Young and old cells treated with WSP or HC were superior to the control in catalase activity with the exception of HC-treated old cells. WSP or HC-treated cells were higher in glu tathione (GSH) concentration than the control with the exception of WSP-treated young cells 4 wk after culturing and HC-treated old cells 6 wk after culturing. Such increases in catalase activity and GSH concentration by WSP treatment may be related to the delay of cellular aging-dependent degeneration.

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Superoxide dismutase specific activities in cultured human diploid cells of various donor ages

Cited by 18 publications

References 20 publications

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

‘The effect of micronutrients on superoxide dismutase in senescent fibroblasts’

Effect of a Radical Scavenger "Water Soluble Protein" from Broad Beans (Vicia faba) on Antioxidative Enzyme Activity in Cellular Aging.

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