Effect of a Radical Scavenger "Water Soluble Protein" from Broad Beans (Vicia faba) on Antioxidative Enzyme Activity in Cellular Aging.

Abstract

SummaryWe isolated a free-radical scavenger "water soluble protein (WSP)" from broad beans. Hydrocortisone (HC) is known to inhibit superoxide generation and was used as the reference scavenger. WSP was examined for its effect on antioxidation in young (PDL 20, 25% of the maximum life span) and old (PDL 50, 62.5% of the maximum life span) human fibroblasts (TIG-1). Cells were treated with WSP or HC for 4 and 6wk in young cells, and for 3 and 6 wk in old cells. The cytosolic superoxide dismutase activity in the cells treated with WSP or HC tended to decrease as compared with that in the non-treated cells (control) with the exception of WSP-treated young cells 4 wk after culturing. Young cells were equal in glutathione peroxidase activity to the control, but the activity level in WSP or HC-treated young cells 6 wk after culturing was 10-50% lower than that in the control. Young and old cells treated with WSP or HC were superior to the control in catalase activity with the exception of HC-treated old cells. WSP or HC-treated cells were higher in glu tathione (GSH) concentration than the control with the exception of WSP-treated young cells 4 wk after culturing and HC-treated old cells 6 wk after culturing. Such increases in catalase activity and GSH concentration by WSP treatment may be related to the delay of cellular aging-dependent degeneration.

Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

“…For example, the presence of HC reversibly mediated growth inhibition as well as anchorage-dependence of rat C6 glioma cells and blocked colony formation in agarose [36,37]. HC-mediated ROS suppression [39,40], decrease of nuclear NF-κB [41], and increases in antioxidant enzymes [42] are likely responsible for such transformation-retarding effects.…”

Lentils, Green and Yellow Split-Peas (Sprouted and Non-Sprouted) on Azoxymethane-Induced Colon Carcinogenesis

“…Dietary lectin has been suggested to inhibit proliferation of colonic cancer cells by inducing cellular differentiation and therefore slows the carcingenesis progression (Madar and Stark, 2002). Another mechanism by which dietary legumes play a role in the chemoprevention is via free radicals scavenging ability of water soluble proteins found in these foods (Okada and Okada, 2000). According to Madar and Stark (2002), these proteins enhance antioxidative capacity in cell lines by increasing catalase activity and glutathione concentrations.…”

Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts

“…21) Okada et al reported that antioxidants with free radical-scavenging activity delayed cellular aging-dependent degeneration by increasing glutathione and catalase activities in human fibroblasts. 43) It was reported that Kangen-karyu extract showed strong anti-aging effects on H 2 O 2 -treated HDFs due to its antioxidative activity and carnosine can delay aging and rejuvenate senescent cultured human fibroblasts. 22,44) Our present data showed that S phase arrest of MRC-5 cells was caused by H 2 O 2 -induced oxidative stress, whereas treatment with the dipeptide attenuated the oxidative status and normalized the cell cycle by decreasing the proportion of cells in the S phase, implying a correlation between a quiescent state of cells, such as growth arrest, and the antioxidant defense system.…”