Superoxide dismutase (SOD) as a selection criterion for triticale grain yield under drought stress: a comprehensive study on genomics and expression profiling, bioinformatics, heritability, and phenotypic variability

Saed-Moucheshi, Armin; Sohrabi, Fatemeh; Fasihfar, Elham; Baniasadi, Fatemeh; Riasat, Mehrnaz; Mozafari, Ali-Akbar

doi:10.1186/s12870-021-02919-5

Cited by 63 publications

(32 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The descriptive and analysis of variance values recorded significant differences among the studied characteristics. Similar results [42] suggested that significant differences were recorded in the studied characteristics under different abiotic stress conditions in barley varieties. Among different types of legumes, Faba bean is salt-sensitive a [43].…”

Section: Discussionsupporting

confidence: 81%

See 1 more Smart Citation

Agronomical and Physiological Responses of Faba Bean Genotypes to Salt Stress

et al. 2022

View full text Add to dashboard Cite

Considering the importance of salinity stress and genotype screening under stress conditions, the current study evaluated faba bean genotypes in response to saline stress and identified those that were tolerant and determined the influential ratio of each yield component on seed yield under both conditions. As a result, 12 faba bean genotypes were tested under 2 levels of salt stress (100 mM and 200 mM) and a control. The study was analyzed with multivariate (descriptive, ANOVA, PCA, biplot, cluster analysis, and indices) analysis techniques to determine the tolerance level of each genotype. Similarly, the cluster analysis results reported that faba bean genotypes were divided into two groups under the control and 100 mM salinity levels; however, the 200 mM salinity level recorded three groups of faba bean genotypes, showing that salinity stress may limit phenotypic variability among faba bean genotypes. The descriptive analysis results showed a wide range of diversity among the studied characteristics under control and salinity stress conditions. The number of seeds/plants recorded a significant association with plant height (cm) (PH), stomatal conductance (SC), days to flowering (DF), the number of pods, and seed weight (g) (SW); however, an insignificant association was recorded with leaf temperature (LT), fresh weight (g) (FW), Na+, K+, and Na/K ratio. The first three principal components (PCs) represent 81.45% of the variance among the studied traits. The most significant characteristics that contributed the most to the diversity were (PH, leaf area, SPAD reading, stomatal conductance, DF, number of pods/plants, number of seeds/pods, SW, K, and total chlorophyll content); however, the significant genotypes (Hassawi-2, Sakha, ILB-4347, Misr-3, FLIP12501FB) were present in PC1 under both conditions. The results predicted that Hassawi-2, ILB-4347, Sakha, Misr-3, and Flip12501FB were the significant (tolerant) genotypes. However, FLIP12504FB represents a sensitive genotype based on its final grain yield. The results of the indices also recorded significant index correlations with grain yield, demonstrating that these indices are effective tools for screening faba bean-tolerant genotypes under salinity stress conditions.

show abstract

Section: Discussionsupporting

confidence: 81%

“…Ahmad et al [43] suggested that sensitive cultivars are more vulnerable to damage relative to tolerance in pea plants and mulberry seedlings [43]. Similar results were reported in Solanum lycopersicum and Vicia faba [42]. Sodium and potassium ions share similar physiochemical structures and compete for Na and K ion uptake in the soil [47].…”

Section: Discussionmentioning

confidence: 76%

Agronomical and Physiological Responses of Faba Bean Genotypes to Salt Stress

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In the present investigation, among the 17 candidate genes, 15 SOD ’s genes in Brassica rapa exhibited a detectable expression genes in at least one of the 6 lesions which are significantly supported with the findings of Feng et al (2015) as investigated in MUSA acuminate. Field experiments and in silico analysis revealed that the superoxide dismutase (SOD) was the most influential antioxidant in resistance of triticale to drought stress; therefore, it could be used as an indirect selection index in early stages to distinguish resistant genotypes to drought stress ( Moucheshi et al, 2021 ). Moreover, Mn-SOD and Fe-SOD showed similar expression levels for both genotypes under drought stress in triticale, but the expression level Cu/Zn-SOD was higher in range in the root and shoot of the tolerant genotype than the susceptible genotype.…”

Section: Discussionmentioning

confidence: 99%

“…Plants have various SOD isozymes according to several research reports, which are grouped as copper/zinc (Cu/Zn-) SOD , manganese (Mn-) SOD , or iron (Fe-) SOD , identified on the basis of metal cofactor, protein folds, and subcellular localization ( Moucheshi et al, 2021 ). These SOD s are found in various cell compartments and these SOD s are found in various cell compartments ( Alscher et al, 2002 , Molina et al, 2013 , Alscher et al, 2002 , Filiz et al, 2014 , Feng et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Insilco identification and characterization of superoxide dismutase gene family in Brassica rapa

Qureshi

Sofi

Dar

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Superoxide Dismutase SODs are defense associated proteins that detoxify ROS and primarily serve as scavengers. They have been described in numerous plant species, but their in-depth characterization in Brassica rapa has not been reported. Therefore, the present investigation on genome wide study of SOD gene family was conducted to identify Br SOD genes, their domain-based organization, gene structure analysis, phylogenetic analysis, intron-exon structure of genes and expression analysis. The sequence characterization of Super oxide dismutase gene family in Brassica rapa , their syntenic associateship of conserved motifs and phylogenetic correlationship, prediction of cis -elements and determing the expression analysis in distinct tissues namely plant callus, root, stem, leaf, flower, and silique under abiotic conditions have been analysed using different software’s. The study on SOD gene family identified 17 Br SOD genes which were grouped into eight Br Cu-ZnSODs and nine Br Fe-MnSODs domain-based organization. Furthermore, the conserved character of Br SODs were confirmed by intron-exon organisation, motif arrangements and domain architectural investigations. Expression analysis using RNA Sequence data of different developmental stages proclaimed that genes were manifested in all six tissues with an exception of Br Cu-ZnSOD3, which was not manifested in roots; however, whose transcript was detected in all other tested tissues. The study has genome wide insight into the occurrence and functional specifications of Br SOD gene family in Brassica rapa that can be potentially utilized in breeding program for resilience to climate change and abiotic stresses tolerance Brassica variety.

show abstract

“…Langroudi et al (2013) showed that drought stress can change essential oils contents of Rosmarinus o cinalis and affect the quantity of its nal production. Saed-Moucheshi et al (2021) stated that drought stress is able to increase the antioxidant contents of triticale as a result of the higher expression rate of related genes.…”

Section: Introductionmentioning

confidence: 99%

The response of Hymenocrater longiflorus to water stress and proline application under in vitro cultivation

Saed-Moucheshi

Mozafari

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Hymenocrater longiflorus Benth. (surahalala) is a wild plant species having properties to be categorized as both pharmaceutical and ornamental plants. To date, the genomics of this plant is unknown and the gene expression profiling of the genes related to its metabolite has never been studied before. In order to study the responses of surahalala plant grown under in vitro conditions to abiotic stresses and the deferential expressions of the genes related to its essential oils under exogenous proline application; three levels of PEG6000 (0, 10, and 20%) and five levels of proline (0, 5, 10, 15, and 20 µM) were applied in mixture with its culture medium. Accordingly, induced water deficit increased oxidant levels while decreased fresh weight of surahalala tissues; whereas, application of proline up to 15 µM was able to relatively compensate the negative effect of water deficit. Contrarily, high proline level (20 µM) showed negative effect surahalala plants which is probably due to the stress simulation (nutrition) in this plant under high proline concentration. Since PEG and proline applications increased essential oil contents, the best combination of proline and PEG treatment in the surahalala plant for achieving the highest content of its essential oils were 10 µM and 10% levels, respectively. The expression profiles of TPS27, L3H, TPS2, TPS1, OMT and GDH3 in this plant were successfully carried out and the results verified the involvement of these genes in 1,8-cineole, carvone, α-pinene, thymol, estragole, and β-Citronellol biosynthesis, respectively. In addition, our results indicated that these genes could get involved in the other metabolite synthesis under water deficit conditions.

show abstract

Superoxide dismutase (SOD) as a selection criterion for triticale grain yield under drought stress: a comprehensive study on genomics and expression profiling, bioinformatics, heritability, and phenotypic variability

Cited by 63 publications

References 53 publications

Agronomical and Physiological Responses of Faba Bean Genotypes to Salt Stress

Agronomical and Physiological Responses of Faba Bean Genotypes to Salt Stress

Insilco identification and characterization of superoxide dismutase gene family in Brassica rapa

The response of Hymenocrater longiflorus to water stress and proline application under in vitro cultivation

Contact Info

Product

Resources

About