Agronomical and Physiological Responses of Faba Bean Genotypes to Salt Stress

Afzal, Muhammad; Alghamdi, Salem S.; Migdadi, Hussein H.; El-Harty, Ehab H.; Al-Faifi, Sulieman A.

doi:10.3390/agriculture12020235

Cited by 17 publications

(14 citation statements)

References 50 publications

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“…Similarly, a recent study on faba bean genotypes was evaluated to determine the salt stress effect on agronomical and physiological response. The results suggested that the salinity affects the agronomical and physiological traits under high (100 mM and 200 mM) salt stress relative to control (Afzal et al 2022a).…”

Section: Effects Of Salinity On Plant Phenological Traitsmentioning

confidence: 89%

Potential Breeding Strategies for Improving Salt Tolerance in Crop Plants

Afzal

Hindawi

Alghamdi

et al. 2022

J Plant Growth Regul

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Salinity is one of the significant abiotic stresses that negatively affect plant production processes, growth, and development, which ultimately reduce yield. Plants adapt specific mechanisms to withstand saline conditions and activate diverse salt tolerance genes to counter osmotic and oxidative stresses induced by salinity. Genetic development in salinity tolerance is quite complex, while advancement has made less progress than expectation over the past few decades. Generating an explosion of genetics- and genomics-related information and technology in recent decades pledge to deliver innovative and advanced resources for the potential production of tolerant genotypes. Despite considerable progress in defining the primary salinity tolerance mechanisms, main obstacles are yet to be solved in the translation and incorporation of the resulting molecular knowledge into the plant breeding activities. Diverse approaches are proposed to enhance plant breeding efficacy to increase plant productivity in saline environments. Understanding the genetics of salt tolerance is a difficult task because multiple genes and pathways are involved. Important advances in tools and methods for updating and manipulating plant genomics knowledge provide detailed insights and dissect the salinity tolerance mechanism accomplished by the breeding goals. Genome-wide analyses (GWA) identify SNP variations and functional effects that appear to be the way of the future for developing salinity-tolerant plants. Gene discovery to manipulate the molecular mechanisms which underlie the complex phenotype of salinity tolerance methods, identification of genes, QTL, association mapping, linkage, and functional genomics, such as transcript identifying and proteins related to salinity, is necessary. The present analysis also discussed some of the opportunities and challenges, focusing on molecular breeding strategies used in conjunction with other crop development approaches to growing elite salt-tolerant lines.

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Section: Effects Of Salinity On Plant Phenological Traitsmentioning

confidence: 89%

Potential Breeding Strategies for Improving Salt Tolerance in Crop Plants

Afzal

Hindawi

Alghamdi

et al. 2022

J Plant Growth Regul

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“…Salinity has a significant impact on yield and productivity in faba beans in KSA. Hassawi-2 (H2) is a salt tolerant genotype most widely cultivated in central and western Saudi Arabia 26 . The current study aims to get deep transcriptome profiling and characterization of DEGs genes under salt stress for different time exposure at early vegetative stage using RNA-Sequencing.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis reveals candidate genes associated with salinity stress tolerance during the early vegetative stage in fababean genotype, Hassawi-2

Afzal,

Alghamdi,

Khan

et al. 2023

Sci Rep

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Abiotic stresses are a significant constraint to plant production globally. Identifying stress-related genes can aid in the development of stress-tolerant elite genotypes and facilitate trait and crop manipulation. The primary aim of this study was to conduct whole transcriptome analyses of the salt-tolerant faba bean genotype, Hassawi-2, under different durations of salt stress (6 h, 12 h, 24 h, 48 h, and 72 h) at the early vegetative stage, to better understand the molecular basis of salt tolerance. After de novo assembly, a total of 140,308 unigenes were obtained. The up-regulated differentially expressed genes (DEGs) were 2380, 2863, 3057, 3484, and 4820 at 6 h, 12 h, 24 h, 48 h, and 72 h of salt stress, respectively. Meanwhile, 1974, 3436, 2371, 3502, and 5958 genes were downregulated at 6 h, 12 h, 24 h, 48 h, and 72 h of salt stress, respectively. These DEGs encoded various regulatory and functional proteins, including kinases, plant hormone proteins, transcriptional factors (TFs) basic helix-loop-helix (bHLH), Myeloblastosis (MYB), and (WRKY), heat shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, dehydrin, antioxidant enzymes, and aquaporin proteins. This suggests that the faba bean genome possesses an abundance of salinity resistance genes, which trigger different adaptive mechanisms under salt stress. Some selected DEGs validated the RNA sequencing results, thus confirming similar gene expression levels. This study represents the first transcriptome analysis of faba bean leaves subjected to salinity stress offering valuable insights into the mechanisms governing salt tolerance in faba bean during the vegetative stage. This comprehensive investigation enhances our understanding of precise gene regulatory mechanisms and holds promise for the development of novel salt-tolerant faba bean salt-tolerant cultivars.

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“…Principle component analysis is a powerful statistical procedure to reduce the dimensions of variables and to divulge constructive evidence-driven feedback from a highly correlated dataset. PCA biplot analysis has been used widely and effectively by other researchers for screening drought-tolerant cultivars of Vicia and lentil species [ 27 , 77 , 78 ]. Current results clearly demonstrated that correlations of a trait pair were well coordinated with the approximation of the vector angles.…”

Section: Discussionmentioning

confidence: 99%

“…Crop wild relatives (CWRs) can play important roles in the improvement of cultivated crops because of exhibiting resistance to various abiotic and biotic stresses [ 26 , 27 , 28 ]. Different genes which are present in CWRs can be utilized to develop new varieties which produce better yield and tolerance [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Wild Vicia Species Possess a Drought Tolerance System for Faba Bean Improvement

Haq

Binjawhar

Ullah

et al. 2022

Genes

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Faba bean (Vicia faba L.), a drought-sensitive crop, is drastically affected by drought stresses compromising its growth and yield. However, wild relatives of faba bean are considered a reservoir of potential genetic resources for tolerance against abiotic stresses. This study was conducted to characterize wild relatives of faba bean for identification of a specific tolerance system required for its improvement against drought stress. The study focused on physiological, biochemical, and anatomical responses of wild Vicia species under drought stress conditions. The experiment was carried out under various levels of drought stress imposed through different field capacities (FC) which included 80% FC ie (well-watered condition), 55% FC (moderate stress), and 30% FC (severe stress). When compared to plants grown in a control environment, drought stress significantly reduced the studied physiological attributes including soluble sugars (21.3% and 15.8%), protein contents (14.7 and 14.6%), and chlorophyll (8.4 and 28.6%) under moderate (55% FC) and severe drought stress (30% FC), respectively. However, proline content increased by 20.5% and 27.6%, peroxidase activity by 48.5% and 57.1%, and superoxide dismutase activity by 72.6% and 64.8% under moderate and severe stress, respectively. The studied anatomical attributes were also affected under drought stress treatments, including diameter of stem xylem vessels (9.1% and 13.7%), leaf lower epidermal thickness (8.05% and 13.34%), and leaf phloem width (5.3% and 10.1%) under moderate and severe stress, respectively. Wild Vicia spp. showed better tolerance to water-deficit conditions as compared to cultivated Vicia L. The observed potential diversity for drought tolerance in wild Vicia spp. may assist in improvement of faba bean and may also help in understanding the mechanisms of adaptations in drought-prone environments.

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Agronomical and Physiological Responses of Faba Bean Genotypes to Salt Stress

Cited by 17 publications

References 50 publications

Potential Breeding Strategies for Improving Salt Tolerance in Crop Plants

Potential Breeding Strategies for Improving Salt Tolerance in Crop Plants

Transcriptomic analysis reveals candidate genes associated with salinity stress tolerance during the early vegetative stage in fababean genotype, Hassawi-2

Wild Vicia Species Possess a Drought Tolerance System for Faba Bean Improvement

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