Superoxide dismutase produced by soil bacteria increases bacterial colony growth from soil samples

Takahashi, Yōko; Katoh, Shogo; Shikura, Noriyasu; Tomoda, Hiroshi; Ōmura, Satoshi

doi:10.2323/jgam.49.263

Cited by 33 publications

(25 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strain KV-614 T was isolated from a soil sample collected at Minato-Ku, Tokyo, Japan, using an agar medium supplemented with SOD following the method of Takahashi et al (2003). Glucose/peptone/meat extract (GPM) agar medium, consisting of 1 % D-glucose (Wako Pure Chem.…”

Section: Methodsmentioning

confidence: 99%

“…For many unknown micro-organisms, the appropriate cultivation conditions have not yet been found. In the course of searching for factors which promote bacterial colony growth, we found that using an isolation agar medium supplemented with superoxide dismutases (SODs) increased the number of colonies isolated from a soil sample, a response that was further enhanced by the addition of catalase (Takahashi et al, 2003). In this paper, we report the taxonomic characterization and classification of strain T isolated from a soil sample using agar medium supplemented with SOD.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov.

Takahashi

Matsumoto

Morisaki

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov. The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, JapanA novel Gram-positive bacterial strain, designated KV-614 T , was isolated from a soil sample using an agar medium supplemented with superoxide dismutase. Based on 16S rRNA gene sequence analysis, it was found that the strain represented a novel deep-rooting lineage within the class Actinobacteria and clustered with yet-uncultivated bacteria from terrestrial environments and some unidentified strains isolated by unique methods. The most closely related established genus was Conexibacter (92?4 % sequence similarity to Conexibacter woesei DSM 14684 T ). Cells of strain T were rod-shaped and motile with long flagella. The strain was catalase-positive, oxidase-negative and grew under aerobic conditions. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine and glutamic acid. The peptidoglycan acyl type was acetyl. The only detected isoprenoid quinone was demethylmenaquinone with seven isoprene units (DMK-7). Mycolic acids were not detected. The predominant cellular fatty acid was v9c-octadecenoic acid (C18 : 1v9c). Minor components were 12-methyl tetradecanoic acid (anteiso-C15 : 0) and 14-methyl hexadecanoic acid (anteiso-C17 : 0). The DNA G+C content was 72 mol%. On the basis of phenotypic and genotypic characteristics, it is proposed that strain KV-614 T represents a new genus and a novel species, Patulibacter minatonensis gen. nov., sp. nov., in the class Actinobacteria. The type strain is KV-614. The creation of the family Patulibacteraceae fam. nov. is proposed to encompass the genus Patulibacter gen. nov. INTRODUCTIONThe number of micro-organisms that have been successfully identified and cultured in vitro represents only a small portion of the total existing in nature; it has been estimated that the number of known bacteria is less than 10 % of the total number of bacterial species in the world (Whitman et al., 1998;Schleifer, 2004). For many unknown micro-organisms, the appropriate cultivation conditions have not yet been found. In the course of searching for factors which promote bacterial colony growth, we found that using an isolation agar medium supplemented with superoxide dismutases (SODs) increased the number of colonies isolated from a soil sample, a response that was further enhanced by the addition of catalase (Takahashi et al., 2003). In this paper, we report the taxonomic characterization and classification of strain T isolated from a soil sample using agar medium supplemented with SOD. METHODSIsolation and cultivation conditions. Strain KV-614 T was isolated from a soil sample collected at Minato-Ku, Tokyo, Japan, using an agar medium supplemented with SOD following the method of Takahashi et al. (2003). Glucose/peptone/meat extract (GPM) agar medium, consisting of 1 % D-glucose (Wako Pur...

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov.

Takahashi

Matsumoto

Morisaki

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…These include the development of a humic acid-vitamin (HV) agar and other methods for the isolation of rare actinomycetes (Hayakawa et al, 1988, Hayakawa, 1990, 1994, 2003, Hayakawa & Nonomura, 1993. Takahashi et al (2003) addressed the problem of oxidative stress in bacteria by the addition of superoxide dismutase and catalase to the isolation medium, and succeeded in obtaining more colonies.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Novel Actinomycetes from Spider Materials

2009

View full text Add to dashboard Cite

To collect new kinds of microorganisms for screening of biologically active substances, we focused on spider materials (webs, cuticle, egg sac), previously uninvestigated sources of such organisms. Using a new method of pre-treatment with 70% ethanol, 1,159 strains of actinomycetes were isolated from 196 spider materials, based on their morphological features. Of these, 293 strains were identified as non-filamentous actinomycetes from their 16S rRNA gene sequences. More detailed examination indicated that 139 strains belonged to the suborders Micrococcineae, Frankineae and Propionibacterineae, and they included some novel strains of non-filamentous actinomycetes. Thus, spider materials provide a more useful source of nonfilamentous actinomycetes than do soil samples.

show abstract

“…The first is isolation of bacteria using agar medium supplemented with superoxide dismutase and catalase. Using this method, we have succeeded in increasing the number of bacterial colonies on isolation plates (Takahashi et al, 2003). The second is isolation of bacterial strains from soil aggregates.…”

mentioning

confidence: 99%

Actinocatenispora sera sp. nov., isolated by long-term culturing

Matsumoto

Takahashi

Fukumoto

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two novel actinomycete strains, KV-744 T and KV-856, were isolated by long-term cultivation. Aerial long-chain spores were produced directly from vegetative mycelia and possessed no motility. Vegetative mycelia developed very well and exhibited fragmentation. The cell-wall peptidoglycan contained meso-diaminopimelic acid, glycine, alanine and glutamic acid, and whole-cell hydrolysates contained arabinose, galactose and xylose. The acyl type of the peptidoglycan was glycolyl. The predominant menaquinone was MK-9(H 4 ) and mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were 14-methylhexadecanoic (ai-C 17 : 0 ), 14-methylpentadecanoic (i-C 16 : 0 ), 15-methylhexadecanoic (i-C 17 : 0 ) and 13-methyltetradecanoic (i-C 15 : 0 ) acids. The G+C content of the DNA was 72-73 mol%. The phenotypic and chemical properties indicated that the two isolates belong to the family Micromonosporaceae and the 16S rRNA gene sequence analysis suggested that the closest relationship was with Actinocatenispora thailandica. The DNA-DNA hybridization values between strain KV-744 T or KV-856 and A. thailandica TT2-10 T were 42-53 %. Based on the data above, strains KV-744 T and KV-856 should be classified as representing a novel species of the genus Actinocatenispora, for which the name Actinocatenispora sera sp. nov. is proposed. The type strain is KV-744 T (5NRRL B-24477 T 5NBRC 101916 T ).Members of the order Actinomycetales offer the possibility of discovering new bioactive compounds. Many new bioactive metabolites have been found from actinomycete strains that were isolated from soil. It is our view that an efficient way of finding new bioactive metabolites is by the discovery of novel micro-organisms, and we have focused on three techniques. The first is isolation of bacteria using agar medium supplemented with superoxide dismutase and catalase. Using this method, we have succeeded in increasing the number of bacterial colonies on isolation plates (Takahashi et al., 2003). The second is isolation of bacterial strains from soil aggregates. The environment for bacteria is known to differ inside and outside soil aggregates (Vargas & Hattori, 1991). We have isolated bacterial strains separately from the inside and outside of soil aggregates and have obtained many isolates (Matsumoto et al., 2006). The third technique is isolation of slow-growing bacteria by long-term cultivation. Janssen et al. (2002) reported that incubation of isolation plates for at least 10 weeks resulted in an increased colony number. We conducted long-term incubation of isolation plates in order to isolate slow-growing bacteria. As a result, strains KV-744 T and KV-856 were isolated using the latter two techniques.The genus Actinocatenispora, members of which produce aerial hyphae bearing spore chains, was proposed by Thawai et al. (2006) as a member of the family Micromonosporaceae. At present, the only recognized species of the genus Actinocatenispora is Actinocatenispora thailandica....

show abstract

Superoxide dismutase produced by soil bacteria increases bacterial colony growth from soil samples

Cited by 33 publications

References 11 publications

Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov.

Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov.

Isolation of Novel Actinomycetes from Spider Materials

Actinocatenispora sera sp. nov., isolated by long-term culturing

Contact Info

Product

Resources

About