Isolation of Novel Actinomycetes from Spider Materials

Iwai, Kimika; Iwamoto, Susumu; Aisaka, Kazuo

doi:10.3209/saj.saj230103

Cited by 10 publications

(8 citation statements)

References 17 publications

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“…In recent years, with the progress in direct 16S rDNA sequencing techniques and the establishment of public-domain data- bases, 16S rDNA sequence comparison has been used successfully for simple identification at the genus or species level in ecological studies of actinomycetes (Wang et al, 1999;Muramatsu et al, 2003;Matsumoto, et al, 2006;Iwai, et al, 2009). In the present investigation, we demonstrated that 16S rDNA-based identification is an efficient method of identification, with 98.5% of 1,234 actinomycete isolates being identified to the genus level and 74.8% being identified to the species level.…”

Section: Discussionmentioning

confidence: 99%

Diversity Analysis of Actinomycetes Assemblages Isolated from Soils in Cool-temperate and Subtropical Areas of Japan

et al. 2010

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The taxonomic diversity of actinomycetes from soils in a cool-temperate area (74 samples from Rishiri Island) and a subtropical area (94 samples from Iriomote Island) of Japan was compared. Soil samples were treated with five different pre-treatment methods and subsequently cultured on humic acid-vitamin agar, resulting in the selective isolation of 1,234 actinomycete strains. These isolates were classified using 16S rDNA sequence analysis. 16S rDNA sequence similarity values of 97% and 98:7% were tentatively used to identify isolates at the genus and species ranks, respectively. The actinomycete assemblages isolated comprised diverse species. Specifically, 668 of the Rishiri isolates fell into 17 families, 40 genera, and 178 species, and 566 of the Iriomote isolates belonged to 21 families, 59 genera, and 194 species. Diversity analyses based on the Shannon index revealed that there was no significant difference between the species diversity of the Rishiri isolates and that of the Iriomote isolates. However, there was little taxonomic overlap between the Rishiri isolates and the Iriomote isolates, as only 66 of the species occurred in both groups. Several species that were reportedly isolated from other tropical areas were found among the Iriomote isolates but not among the Rishiri isolates. This result suggests that climate may influence the species types of soil-inhabiting actinomycetes.

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Section: Discussionmentioning

confidence: 99%

Diversity Analysis of Actinomycetes Assemblages Isolated from Soils in Cool-temperate and Subtropical Areas of Japan

et al. 2010

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“…Actinobacteria associated with insect hosts are a very promising source of providing novel active small molecules that confer benefits to hosts [1][2][3][4]. The insect inner gut system is an important natural environment harboring diverse microbes that may produce bioactive substances.…”

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confidence: 99%

Cytotoxic Angucyclines fromAmycolatopsissp. HCa1, a Rare Actinobacteria Derived fromOxya chinensis

Zhong¹,

Wang²,

Guo³

et al. 2011

Planta Med

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Two new angucyclines, named (2R,3R)-2-hydroxy-8- O-methyltetrangomycin (1) and (2R,3R)-2-hydroxy-5-O-methyltetrangomycin (2), together with eight known compounds (3-10), were isolated from the culture of Amycolatopsis sp. HCa1, a rare actinobacteria isolated from the gut of Oxya chinensis. The new structures were elucidated through extensive spectroscopic data analysis, and their absolute configurations were assigned by application of the modified Mosher's method and CD spectrum comparison. Their IN VITRO cytotoxic activities against four cell lines including human cervical cancer cell line (HeLa), human gastric adenocarcinoma cell line (SGC-7901), human lung adenocarcinoma cell line (SPC-A-1), and mouse macrophage cell line (RAW264.7) were then investigated. Compounds 3, 4, 9, and 10 showed potent cytotoxic activities towards the HeLa cells with IC (50) values of 0.27, 0.11, 0.56, and 0.39 µM, respectively.

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“…Secretary products of actinomycetes of rare ecosystems are meant to antagonistic to test pathogens are likely to be novel anti-mycobacterial compounds as they new to human pathogens. Analysis of 16S rDNA and the repetitive extragenic palindromic (REP)-PCR fingerprinting technique (25) have been suggested for characterization and classification of environmental actinomycetes. In recent years, with the progress in direct 16S rDNA sequencing techniques and the establishment of publicdomain data bases, 16S rDNA sequence comparison has been used successfully for simple identification at the genus or species level in ecological studies of actinomycetes (26).…”

Section: Characterization Of Active Strainmentioning

confidence: 99%

Selective isolation and characterization of rare actinomycetes adopted in glacier soil of Manali ice point and its activity against Mycobacterium spp

A¹,

Gajalakshmi

2017

J Microbiol Biotech Res

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An exigent demand of antimicrobial agent against M. tuberculosis was lead to the isolation of novel rare actinomycetes from the unexplored cryophilic environment. Soil samples were collected from glacier ice point of Kullu Manali and processed for further studies. A novel approach was described for the isolation of rare actinomycetes from a heterogeneous population. Isolation was done by conventional and density gradient centrifugation. Sucrose gradient centrifugation showed a maximum of 24 actinomycetes isolates which belong to the genera of Streptomyces sp (12), Micromonospora sp (5) Planomonospora sp(2), Micropolyspora sp (2), Actinopolyspora sp (1) Nocardia sp (1) and Intrasporangium sp (1). Of these 24 actinomycetes, isolate Planomonospora sp (PL-2) showed potent anti-mycobacterial activity against Mycobacterium smegmatis (MTCC300) and M.tuberculosis (MTCC 6). Bioautography reveals that the Rf value of active compound was 0.75 and retains the antimicrobial activity at 75° C. Based on the C 13 and H 1 NMR the active compound was characterized as 2-(2-ethenylphenyl) heptane-1-ol. Phylogenetic analysis reveals active isolate was closely related to Planomonospora alba and the Genbank accession is JQ280498.Key Words: Extremophiles, cryophilic, Planomonospora sp, planosporin, Mycobacterium tuberculosis.HOW TO CITE THIS ARTICLE Raja A and Gajalakshmi P, Selective isolation and characterization of rare actinomycetes adopted in glacier soil of Manali ice point and its activity against Mycobacterium spp, J Microbiol Biotech Res, 2017, 7 (5): 1-10,

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Isolation of Novel Actinomycetes from Spider Materials

Cited by 10 publications

References 17 publications

Diversity Analysis of Actinomycetes Assemblages Isolated from Soils in Cool-temperate and Subtropical Areas of Japan

Diversity Analysis of Actinomycetes Assemblages Isolated from Soils in Cool-temperate and Subtropical Areas of Japan

Cytotoxic Angucyclines fromAmycolatopsissp. HCa1, a Rare Actinobacteria Derived fromOxya chinensis

Selective isolation and characterization of rare actinomycetes adopted in glacier soil of Manali ice point and its activity against Mycobacterium spp

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