Superoxide anion production and some sperm-specific enzyme activities in infertile men

Abstract

The relationship between superoxide anion production and the activities of sperm specific NADH-diaphorase and isozyme LDH-C, has been investigated in 25 normozoospermic and 17 oligozoospermic semen samples. The samples were separated into high and low density sperm populations on discontinuous, two step (40%:80%) Percoll gradients prior to analysis. Superoxide anion generation was significantly higher (P< 0.001) and diaphorase activity significantly increased (P<0.01) in both low and high density sperm fractions from oligozoospermic patients compared to the values found in normozoospermic men. A significant positive correlation ( P < 0.0 1) between diaphorase activity and superoxide anion production was established in the oligozoospermic samples of low density sperm fraction. A tendency of statistically insignificant increase in LDH-C, isozyme activity was observed in the oligozoospermic samples. The activity of this isozyme appears to have no association with superoxide anion generation or diaphorase activity. These findings seem to suggest that excessive superoxide anion production along with increased diaphorase activity point to biochemical abnormalities which may affect the functional status of sperms. Hence, determination of these parameters may provide useful data in male infertility of unknown etiology.

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

“…Our findings have shown that superoxide anion levels were higher in infertile men with abnormal semen parameters compared with normal group, and this is in accordance with the results of other studies (Gavella et al, 1995;Said et al, 2004). In asthenooligoteratozoospermic patients, the level of superoxide anion was the highest compared with other infertile groups.…”

Relationship of sperm superoxide dismutase-like activity with other sperm-specific enzymes and experimentally induced lipid peroxidation in infertile men

“…It was previously established that after TritonX-100 treatment, both the soluble and membrane-bound diaphorase were measured (Gavella et al, 1995). For lactate dehydrogenase-C4 isoenzyme and NADH diaphorase activity determination, spermatozoa were permeabilized with 0.2% TritonX-100 (Sigma, St. Louis, MO, USA).…”

“…For lactate dehydrogenase-C4 isoenzyme and NADH diaphorase activity determination, spermatozoa were permeabilized with 0.2% TritonX-100 (Sigma, St. Louis, MO, USA). It was previously established that after TritonX-100 treatment, both the soluble and membrane-bound diaphorase were measured (Gavella et al, 1995). TritonX-100 treatment was also employed for LDH-C4 isoenzyme activity determination, as proposed by Casano et al (1991).…”

“…lactate dehydrogenase-C4 isoenzyme (Casano et al, 1991;Orlando et al, 1994) and NADH-diaphorase (Gavella & Lipovac, 1992, Gavella et al, 1995, and (ii) to investigate the physiological relevance of SODlike activity in human spermatozoa, by examining its relationship with experimentally-induced lipid peroxidation. lactate dehydrogenase-C4 isoenzyme (Casano et al, 1991;Orlando et al, 1994) and NADH-diaphorase (Gavella & Lipovac, 1992, Gavella et al, 1995, and (ii) to investigate the physiological relevance of SODlike activity in human spermatozoa, by examining its relationship with experimentally-induced lipid peroxidation.…”

“…The objective of this study was (i) to measure SOD-like activity in the spermatozoa of normoand oligozoospermic men, in order to detect possible relationships between SOD-like activity in human spermatozoa and enzymatic markers of spermatozoa maturity, e.g. lactate dehydrogenase-C4 isoenzyme (Casano et al, 1991;Orlando et al, 1994) and NADH-diaphorase (Gavella & Lipovac, 1992, Gavella et al, 1995, and (ii) to investigate the physiological relevance of SODlike activity in human spermatozoa, by examining its relationship with experimentally-induced lipid peroxidation. (WHO, 1992) and the hypo-osmotic swelling test (HOST) was used for the evaluation of the functional integrity of sperm membrane (Jeyendran et al, 1984).…”