Superovulation, in vitro fertilization (IVF) and in vitro development (IVD) protocols for inbred BALB/cJ mice in comparison with outbred NMRI mice

Golkar‐Narenji, Afsaneh; Gourabi, Hamid; Eimani, Hussein; Barekati, Zeinab; Akhlaghi, Ali Asghar

doi:10.1007/s12522-012-0127-8

Cited by 8 publications

(9 citation statements)

References 48 publications

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“…In contrast to previous reports, 8,27,44 we show here that VDD alone (without hypocalcemia) did not reduce sperm cell motility, regardless of the severity of VDD. We therefore reinforce the idea that the previously reported reduction in sperm motility in VDD cases was likely a calcium‐mediated effect, as suggested 8,27‐49 . The results of our experiments are therefore in complete agreement with Sun et al 27 who demonstrated that VDD is accompanied by decreased extracellular calcium and phosphorus levels and that defective sperm motility in these situations can be overcome by calcium and phosphorus supplements.…”

Section: Discussionsupporting

confidence: 93%

“…For induction of superovulation, female mice (weight 25‐30 g) received 10 IU of pregnant mare's serum gonadotropin (PMSG; Pregnecol, Armidale, Australia). After 48 hours, they were treated with 10 IU of human chorionic gonadotropin (hCG; Pregnyl, Tehran, Iran) 31 . In addition, in each group, five superovulated plugged positive females were used on day 3.5 after hCG injection to assess in vivo rate of blastocyst formation.…”

Section: Methodsmentioning

confidence: 99%

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Impact of vitamin D deficiency on mouse sperm structure and function

et al. 2020

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Background In rodents and humans, vitamin D deficiency (VDD) is associated with altered sperm structure and function (primarily decreased motility and morphological abnormalities) that are primarily attributed to VDD‐induced hypocalcemia. However, it is suspected that VDD has much more drastic effects on mammalian spermatozoa. Objectives The purpose of this study was to illustrate that VDD, depending on its severity and duration, can alter sperm nuclear integrity and can also lead to the loss of spermatozoa's ability to support embryonic development. Materials and methods A mouse model of induced VDD combining the action of a vitamin D‐deficient diet, UV exposure limitation, and paricalcitol injections; a vitamin D2 analog that catabolizes endogenous vitamin D by increasing the expression of CYP24A, a member of the cytochrome P450 family, has been used to create different grades of VDD. Results We show that the most significant sperm defect recorded concerns the integrity of the paternal nucleus, which is both decondensed and fragmented in moderate‐to‐severe VDD situations. Consistent with the known consequences of fertilization with DNA‐damaged spermatozoa, we show that paternal VDD decreases the ability of spermatozoa to optimally support fertilization and embryonic development. Discussion and Conclusion Given the worldwide high prevalence of VDD in humans, and although obtained in an animal model, the data presented here suggest that subfertile/infertile males may benefit from VDD testing and that attempts to correct serum vitamin D levels could be considered prior to conception, either naturally or through ART.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Impact of vitamin D deficiency on mouse sperm structure and function

et al. 2020

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“…At the end of the treatment period (day 21), mature NMRI female mice were superovulated by intraperitoneal injection of 7.5 IU pregnant mare serum gonadotropin (PMSG) (Sigma‐Aldrich) followed by the administration of 7.5 IU human chorionic gonadotropin (HCG) (Sigma‐Aldrich) 48 hours later . Ovulation occurred within 14 hours of post‐HCG injection.…”

Section: Methodsmentioning

confidence: 99%

Upregulation of FSHR and PCNA by administration of coenzyme Q10 on cyclophosphamide‐induced premature ovarian failure in a mouse model

Delkhosh

Delashoub

Tehrani

et al. 2019

J Biochem & Molecular Tox

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Cyclophosphamide (CTX) has been broadly used in the clinic for the treatment of autoimmune disorders and ovarian cancer. The process of chemotherapy has significant toxicity in the reproductive system as it has detrimental effects on folliculogenesis, which leads to an irreversible premature ovarian failure (POF).Coenzyme Q10 (CoQ10) has positive impacts on the reproductive system due to its antioxidant properties, protecting the cells from free-radical oxidative damage and apoptosis. However, little is known about the possible synergistic effect of CTX and CoQ10 on the expression of genes involved in folliculogenesis, such as proliferation cell nuclear antigen (PCNA) and follicle-stimulating hormone receptor (FSHR). A total of 32 NMRI mice were applied and divided into four groups, including healthy control, CTX, CTX + CoQ10, and CoQ10 groups. The effects of CoQ10 on CTX-induced ovarian injury and folliculogenesis were examined by histopathological and real-time quantitative reverse transcription-polymerase chain reaction analyses. The rates of fertilization (in vitro fertilization), embryo development, as well as the level of reactive oxygen species (ROS) in metaphase II (MII) mouse oocytes after PMSG/HCC treatment were also assessed. Results showed that the treatment with CTX decreased the mRNA expression of PCNA and FSHR, IVF rate, and embryo development whereas the application of CoQ10 successfully reversed those factors.CoQ10 administration significantly enhanced histological morphology and decreased ROS levels and the number of atretic follicles in the ovary of CTX-treated mice. In conclusion, it seems that the protective effect of CoQ10 is exerted via the antioxidant and proliferative properties of this substance on CTX-induced ovarian damage.

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“…Our results are, however, compatible with those described by Van der Auwera et al [23], reinforcing that the superovulation impact occurs not only at a later stage of development but also that the embryo reduction occurs soon after superovulation. Genetic alterations among the strains used may also contribute to variable responses to superovulation, fertilization levels [34] [35] and incubation rates. Fertilization rates can be easily determined when embryos are collected at 0.5 dpc by considering the total number of oocytes/1-cell embryos and allowing passage to a 2-cells stage or detecting pronucleus under the microscope.…”

Section: Discussionmentioning

confidence: 99%

Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains

Lamas¹,

Franquinho²,

Morgado³

et al. 2020

OJAS

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Superovulation is used to stimulate the production and release of large amounts of oocytes in mice by using two hormones that mimic FSH (PMSG) and LH (hCG) effects. Since superovulation can have a negative impact on oocyte and embryo development, this investigation aimed to compare two alternatives for 2-cells embryo collection in order to reduce the number of females and to benefit from the superovulation process. Data from mouse embryo collection from our facility was analyzed to compare the number of 2-cells embryos collected at 1.5 dpc and the number of 2-cells embryos obtained after overnight incubation of 1-cell embryos, collected at 0.5 dpc. Genetically modified mouse strains with a similar background (C57BL/6J, B6*129 and FVB/NJ) were analyzed and for strains at a C57BL/6J and B6*129 background, the number of 2-cells embryos obtained after incubation was significantly higher when compared to the number of 2-cells embryos collected at 1.5 dpc (1.4-fold and 1.7-fold, respectively). C57BL/6J wild type mice had similar results with a higher number of 2-cells embryos when collection was performed at 0.5 dpc followed by incubation (1.4-fold). These results can help the planning of 2-cells embryo harvesting by reducing the number of females needed for this procedure.

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Superovulation, in vitro fertilization (IVF) and in vitro development (IVD) protocols for inbred BALB/cJ mice in comparison with outbred NMRI mice

Cited by 8 publications

References 48 publications

Impact of vitamin D deficiency on mouse sperm structure and function

Impact of vitamin D deficiency on mouse sperm structure and function

Upregulation of FSHR and PCNA by administration of coenzyme Q10 on cyclophosphamide‐induced premature ovarian failure in a mouse model

Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains

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