Superiority of plasma 11-dehydro-TXB2 to TXB2 as an index of in vivo TX formation in rabbits after dosing of CS-518, A TX synthase inhibitor

Takasaki, Wataru; Nakagawa, Akihiko; Asai, Fumitoshi; Ushiyama, Shigeru; Sugidachi, Atsuhiro; Matsuda, Kazuo; Oshima, Takeshi; Tanaka, Yorihisa

doi:10.1016/0049-3848(93)90206-4

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…In this study, we could not find any effect of diet on the plasma level of 11-dehydrothromboxane B 2 which is a marker of the systemic production of thromboxane A 2 . Takasaki et al (32) have reported that the plasma level of 11-dehydrothromboxane B 2 is a useful marker of in vivo thromboxane formation in rabbits.…”

Section: Discussionmentioning

confidence: 99%

The effects of dietary α‐linolenic acid compared with docosahexaenoic acid on brain, retina, liver, and heart in the guinea pig

Abedin

Lien²,

Vingrys

et al. 1999

Lipids

114

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The aim of this study was to compare two different strategies to elevate brain, retina, liver, and heart docosahexaenoic acid (DHA) levels in guinea pigs. First, we used an increasing dose of alpha-linolenic acid (ALA) relative to a constant linoleic acid (LA) intake, and second, we used two levels of dietary DHA provided in conjunction with dietary arachidonic acid (AA). The percentage DHA and AA of total phospholipids in retina, liver, and heart, and in the brain phosphatidylethanolamine and phosphatidylcholine was studied in female pigmented guinea pigs (3 wk old) fed one of five semisynthetic diets containing 10% (w/w) lipid for 12 wk. The LA content in the diets was constant (17% of total fatty acids), with the ALA content varying from 0.05% (diet SFO), to 1% (diet Mix), and to 7% (diet CNO). Two other diets (LCP1 and LCP3) had a constant LA/ALA ratio (17.5:1) but varied in the levels of dietary AA and DHA supplementation. Diet LCP1 was structured to closely replicate the principal long chain polyunsaturated fatty acids (PUFA) found in human breast milk and contained 0.9% AA and 0.6% DHA (% of total fatty acids) whereas diet LCP3 contained 2.7% AA and 1.8% DHA. At the end of the study, animals were sacrificed and tissues taken for fatty acid analyses. We found no significant effects of diets on the growth of guinea pigs. Diets containing ALA had profoundly different effects on tissue fatty acid compositions compared with diets which contained the long chain PUFA (DHA and AA). In the retina and brain phospholipids, high-ALA diets or dietary DHA supplementation produced moderate relative increases in DHA levels. There was no change in retinal or brain AA proportions following dietary AA supplementation, even at the highest level. This was in contrast to liver and heart where tissue DHA proportions were low and AA predominated. In these latter tissues, dietary ALA had little effect on tissue DHA proportions although the proportion of AA was slightly depressed at the highest dietary ALA intake, but dietary DHA and AA supplements led to large increases (up to 10-fold) in the proportions of these PUFA. Tissue uptake of dietary AA and DHA appeared maximal for the LCP1 diet (replicate of breast milk) in the heart. There were no significant changes in the plasma levels of 11-dehydrothromboxane B2 (a thromboxane A2 metabolite) for any diet. The data confirm that dietary ALA is less effective than dietary DHA supplementation (on a gram/gram basis) in increasing tissue DHA levels and that tissues vary greatly in their response to exogenous AA and DHA, with the levels of these long chain metabolites being most resistant to change in the retina and brain compared with liver and heart. Dietary DHA markedly increased tissue DHA proportions in both liver and heart, whereas the major effect of dietary AA was in the liver. Future studies of the effects of dietary DHA and AA supplementation should examine a variety of tissues rather than focusing only on neural tissue.

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Section: Discussionmentioning

confidence: 99%

The effects of dietary α‐linolenic acid compared with docosahexaenoic acid on brain, retina, liver, and heart in the guinea pig

Abedin

Lien²,

Vingrys

et al. 1999

Lipids

114

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Evaluation of plasma 11-dehydro-thromboxane B2 as an indicator for thromboxane A2 synthesis in vivo in laboratory animals

Kawamura

Harada

Kikuchi

1995

Thrombosis Research

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Original Article: Intracellular Ca²⁺Mobilization and Aggregatory Response to ADP and Thrombin in Aged Rat Platelets

Sugidachi¹,

Asai²,

Oshima³

et al. 1995

Platelets

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We previously reported that thrombin-induced Ca(2+) mobilization was enhanced in aged rat platelets. Since Ca(2+) mobilization in platelets is believed to be closely associated with platelet activation, we examined Ca(2+) mobilization and the aggregatory response to ADP and thrombin in young (3 months) and aged (24 months) rat platelets. Blood levels of fibrinogen and Ca(2+) in aged rats were higher than those in young rats. ADP-induced platelet aggregation in aged rats was significantly enhanced in platelet rich plasma and in washed platelet suspension, suggesting that age-associated hyperaggregability to ADP is attributable to changes in platelets themselves. On the other hand, thrombin (0.03-0.3 unit/ml)-induced aggregation in washed platelet suspension from aged rats was comparable to that from young rats. But, thrombin (0.3 unit/ml)-induced intracellular Ca(2+)mobilization was enhanced in aged rat platelets in the presence of extracellular Ca(2+). Likewise, ADP-induced Ca(2+) mobilization was enhanced in aged rat platelets. These results suggest that enhanced Ca(2+) mobilization in aged rat platelets is associated with hyperaggregability to ADP but not to thrombin.

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Superiority of plasma 11-dehydro-TXB2 to TXB2 as an index of in vivo TX formation in rabbits after dosing of CS-518, A TX synthase inhibitor

Cited by 3 publications

References 13 publications

The effects of dietary α‐linolenic acid compared with docosahexaenoic acid on brain, retina, liver, and heart in the guinea pig

The effects of dietary α‐linolenic acid compared with docosahexaenoic acid on brain, retina, liver, and heart in the guinea pig

Evaluation of plasma 11-dehydro-thromboxane B2 as an indicator for thromboxane A2 synthesis in vivo in laboratory animals

Original Article: Intracellular Ca²⁺Mobilization and Aggregatory Response to ADP and Thrombin in Aged Rat Platelets

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Superiority of plasma 11-dehydro-TXB2 to TXB2 as an index of in vivo TX formation in rabbits after dosing of CS-518, A TX synthase inhibitor

Cited by 3 publications

References 13 publications

The effects of dietary α‐linolenic acid compared with docosahexaenoic acid on brain, retina, liver, and heart in the guinea pig

The effects of dietary α‐linolenic acid compared with docosahexaenoic acid on brain, retina, liver, and heart in the guinea pig

Evaluation of plasma 11-dehydro-thromboxane B2 as an indicator for thromboxane A2 synthesis in vivo in laboratory animals

Original Article: Intracellular Ca2+Mobilization and Aggregatory Response to ADP and Thrombin in Aged Rat Platelets

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Original Article: Intracellular Ca²⁺Mobilization and Aggregatory Response to ADP and Thrombin in Aged Rat Platelets