Evaluation of plasma 11-dehydro-thromboxane B2 as an indicator for thromboxane A2 synthesis in vivo in laboratory animals

Kawamura, Michiko; Harada, Yoshiteru; Kikuchi, Makoto

doi:10.1016/0049-3848(95)93882-z

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…11-DH-TXB 2 is the metabolite of TXB 2 , and the plasma level of 11-DH-TXB 2 has been suggested to be an indicator of TXA 2 production in humans. 18 – 20 Aspirin activity and antiplatelet action can be evaluated by measurement of the end-products of the TXA 2 pathway, such as 11-DH-TXB 2 . The 11-DH-TXB 2 levels also reflect the amount of TXA 2 derived from other sources besides platelets, such as macrophages and monocytes.…”

Section: Discussionmentioning

confidence: 99%

Immature platelets and antiplatelet therapy response to aspirin in Kawasaki disease

Che

Long

et al. 2018

DDDT

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IntroductionKawasaki disease is a kind of systemic vasculitis that mainly damages moderate and small-sized blood vessels, and is a leading cause of coronary artery lesions (CAL). Antiplatelet therapy is a routine component of Kawasaki disease treatment strategies. So it is important to evaluate the antiplatelet effect of aspirin because of the individual biological variability of antiplatelet effect of aspirin. The immature platelet fraction (IPF) has attracted particular attention as it may influence the antiplatelet effect of aspirin. This study investigated the prognostic factors for evaluating the degree of vasculitis and the effect of antiplatelet therapy in children with Kawasaki disease.Materials and methodsBlood samples were collected from 44 patients with Kawasaki disease before aspirin treatment and 7 to 10 days after treatment. The IPF counts, percentage of the IPF, and highly fluorescent IPF were detected by a Sysmex XE-5000 instrument. The levels of 11-dehydrothromboxane B2 (11-DH-TXB2), soluble CD40 ligand (sCD40L), and soluble P-selectin (sP-selectin) were measured by ELISA. The correlation between the measured factors and the degree of coronary artery damage in Kawasaki disease was analyzed.ResultsWe found that 11-DH-TXB2, sP-selectin, and sCD40L levels were much more elevated in the CAL group than in the non-coronary artery lesions (NCAL) group before aspirin treatment. The concentrations of 11-DH-TXB2, sCD40L, sP-selectin, and IPF were reduced after aspirin treatment in the NCAL group but not the CAL group. This is related to the degree of coronary artery damage in Kawasaki disease patients. Additionally, 11-DH-TXB2, sCD40L, sP-selectin, and IPF were positively correlated with the degree of coronary artery damage in Kawasaki disease patients.ConclusionThe current study suggests that the presence of high plasma concentrations of 11-DH-TXB2, sCD40L, sP-selectin, and IPF can be considered a risk factor and experimental biomarker for CAL in Kawasaki disease patients.

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Section: Discussionmentioning

confidence: 99%

Immature platelets and antiplatelet therapy response to aspirin in Kawasaki disease

Che

Long

et al. 2018

DDDT

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Targeting the urine and plasma determinants of thromboxane A2 metabolism in detection of aspirin effectiveness

Dobaczewski

Nocuń

Заводник

et al. 2008

Blood Coagulation &Amp; Fibrinolysis

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Detection of reduced aspirin effectiveness has gained significant importance since clinical consequences of aspirin resistance were reported. Nevertheless, due to differentiated molecular basis of aspirin resistance, the conflicting choice of referential method for detection of acetylsalicylic acid ineffectiveness has become troublesome. This study, using a rat model of antiplatelet therapy, examines the aptitude of selected TXB2 metabolism-based methods in the detection of acetylsalicylic acid effectiveness. We hypothesized that ex-vivo whole blood spontaneous TXB2 generation assay could be, contrary to basal TXB2 and urine 11-dTXB2, a novel surrogate measure for impaired acetylsalicylic acid-dependent inhibition of thromboxane synthesis. To address this hypothesis, we evaluated the sensitivity of TXB2 generation assay in hirudinized whole blood to detect acetylsalicylic acid-mediated inhibition of cyclooxygenase activity in healthy rats and diabetic rats treated with acetylsalicylic acid. In diabetic and control animals, both acetylsalicylic acid drenches in the dose-independent manner contributed to significant attenuation of basal plasma TXB2 and urinary 11-dTXB2 formation. Urinary concentrations of 11-dTXB2 were, contrary to basal TXB2, significantly higher, regardless of acetylsalicylic acid dose, among all diabetic groups, compared with corresponding control groups. Determination of TXB2 generation in whole blood enabled sensitive detection of dose-related acetylsalicylic acid effect in both groups, as well as increased TXB2 formation in diabetes. We showed for the first time that evaluation of spontaneous generation of TXB2 in hirudinized whole blood enables, contrary to basal plasma TXB2 and urine 11-dTXB2 concentrations, to sensitively determine the acetylsalicylic acid effect in healthy and diabetic subjects.

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Evaluation of effects of low-dose aspirin administration on urinary thromboxane metabolites in healthy dogs

Hoh¹,

Smith²,

McMichael³

et al. 2011

ajvr

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Evaluation of plasma 11-dehydro-thromboxane B2 as an indicator for thromboxane A2 synthesis in vivo in laboratory animals

Cited by 5 publications

References 20 publications

Immature platelets and antiplatelet therapy response to aspirin in Kawasaki disease

Immature platelets and antiplatelet therapy response to aspirin in Kawasaki disease

Targeting the urine and plasma determinants of thromboxane A2 metabolism in detection of aspirin effectiveness

Evaluation of effects of low-dose aspirin administration on urinary thromboxane metabolites in healthy dogs

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