Superior Antioxidant Capacity of Berberis iliensis—HPLC-Q-TOF-MS Based Phytochemical Studies and Spectrophotometric Determinations

Abstract

The aim of the present study was to determine the composition, antiradical and antimicrobial activity of fruits, leaves and roots of an underestimated species of barberry—Berberis iliensis—growing in Kazakhstan. Particular attention was paid to the determination of the composition of its extracts by high-performance liquid chromatography coupled with mass spectrometry (HPLC-ESI-Q-TOF-MS) analysis. As a result of the chromatographic and spectrometric study 33 secondary metabolites from the groups of phenolic acids and their esters, flavonoids, alkaloids and organic acids were identified and 15 of them—quantified. The isomers of caffeoyl-glucaric acid, caffeic acid derivatives, isoquercetin, berberine and jatrorrhizine were the most abundant components of the tested extracts. The antiradical activity tests were performed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Folin-Ciocalteu assays on four types of extracts (water, ethanol, ethanol-water 7:3 v/v, ethanol-water 1:1 v/v) from the three organs of the plant. The highest antiradical potential (IC50 = 80 ± 6.36 µg/mL) and phenolic content (440 ± 17.1 mg gallic acid equivalents/L) was calculated for ethanol- water (1:1 v/v) extracts from the leaves and could be influenced by the abundant presence of simple phenolic acids, flavonoids and glucaric acid esters. Among reference microorganisms, M. luteus, S. epidermidis, some S. aureus and B. cereus belonging to Gram-positive bacteria and yeasts from Candida species were the most sensitive to roots extract that was found the most active among the studied samples. The results of the study classify Berberis iliensis as a strong antioxidant agent and as a plant with an antimicrobial potential.

Influence of Accelerated Solvent Extraction Conditions on the LC-ESI-MS/MS Polyphenolic Profile, Triterpenoid Content, and Antioxidant and Anti-lipoxygenase Activity of Rhododendron luteum Sweet Leaves

“…However, a significant correlation with the catechin level was noticed (0.663). Our observations are in agreement with previous papers, where many polyphenolic metabolites were described to be highly effective radical scavengers [ 62 ].…”

Wild plants used by the Lhoba people in Douyu Village, characterized by high mountains and valleys, in southeastern Tibet, China

“…The uses of fresh fruit are similar to those for other Berberis species in the Himalayas [11,15,50,51]. This genus is also rich in pharmacologically active compounds, such as berberine, berbamine, palmatine, oxyacanthine, and jatrorrhizine [52]. Berberine is widely used to treat diarrhea by inhibiting intestinal inflammation and regulating intestinal bacteria [53][54][55][56][57].…”

Study of the Potential Antiulcerous Action of Hydroalcoholic Extracts From Prunella Vulgaris L. Of Romanian Origin

“…We extracted 5 g of plant product with 50 mL of solvent, then 0.5 mL of the sample was diluted to 10 mL, the diluted sample being used in the determinations. The preparation of standards and samples for analysis was carried out in dark-coloured bottles, protected from light, using a methodology similar to Abdykerimova et al [2]. Thus, 40 μL of sample or standard solution were diluted with 3160 µL of distilled water; then 200 μL of Folin Cocâlteu reagent and 600 μL of 20% NaHCO3 were added.…”