Superheated water chromatography on phenyl bonded hybrid stationary phases

Al-Khateeb, Lateefa A.; Smith, Roger M.

doi:10.1016/j.chroma.2008.06.013

Cited by 20 publications

(11 citation statements)

References 25 publications

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“…In order to avoid very long retention times not every combination was examined. The results for some of these analytes in 100% water have been reported previously [12]. As expected there were marked increases in the retention factors as the proportion of methanol was lowered.…”

Section: Absorbance (Mau)supporting

confidence: 59%

“…However, although water is environmentally friendly, cheap and non-hazardous, even at 200°C it is only a moderately strong solvent and is often unable to rapidly elute relatively non-polar organic compounds, such as steroids [12] which can lead to long-retention times. This has lead to an interest in the use of low proportion of organic modifier in the eluent.…”

Section: Introductionmentioning

confidence: 99%

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Elevated Temperature Separations on Hybrid Stationary Phases with Low Proportions of Organic Modifier in the Eluent

Khateeb

Smith

2011

Chromatographia

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The properties of two thermally stable organic/ inorganic hybrid columns, XTerra MS C 18 and XTerra phenyl were examined at elevated temperatures, using a number of test compounds, including acetophenone derivatives and phenols, with 5 and 10% methanol in water as the mobile phases. The thermodynamics of the separations were examined. The study demonstrated that a low proportion of organic modifier and moderate temperatures gave similar separations to higher temperature with superheated water, potentially reducing stress on the column and analytes.

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Section: Absorbance (Mau)supporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Elevated Temperature Separations on Hybrid Stationary Phases with Low Proportions of Organic Modifier in the Eluent

Khateeb

Smith

2011

Chromatographia

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“…A variety of stationary phases including polysterene-, polystyrene-divinylbenzene-, zirconium dioxide-, titanium dioxide-, and carbon-based phases as well as selected silica-based reversed phases (e.g., Zorbax StableBond C18 and Zorbax RX-C 8 from Agilent Technologies, XBridge BEH from Waters, various fused-core phases) demonstrated acceptable stability at 150-200°C for at least several hundred hours [47,[64][65][66][67]. In this work, we used either a polar-embedded C18 stationary phase (Magic C18 AQ, Michrom Bioresources, MA) or in-house polymerized highly crosslinked polystyrene-divinylbenezene monolithic phase.…”

Section: Resultsmentioning

confidence: 99%

Comparative studies of peak intensities and chromatographic separation of proteolytic digests, PTMs, and intact proteins obtained by nanoLC-ESI MS analysis at room and elevated temperatures

Moskovets

Ivanov

2016

Anal Bioanal Chem

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This work demonstrates that the chromatographic separation performed at highly stabilized elevated temperature results in significant improvements in sensitivity, quantitative accuracy, chromatographic resolution, and run-to-run reproducibility of nanoLC-MS analysis of complex peptides mixtures. A newly developed platform was shown to provide conditions for accurate temperature stabilization and temperature homogeneity when performing nanoLC-ESI MS analysis. We quantitatively assessed and compared the recovery of peptides and small proteins from nanoLC columns at room and elevated temperatures. We found that analyses performed at highly stabilized elevated temperatures led to improved detection sensitivity, reproducibility, and chromatographic resolution in reversed-phase LC separation of unmodified peptides (both hydrophilic and hydrophobic), post-translationally modified peptides (O-phosphorylated), and small intact proteins. The analytical benefits of elevated temperatures for qualitative and quantitative proteomic LC-MS profiling were demonstrated using mixtures of synthetic peptides, tryptic digests of mixtures of model proteins, and digested total lysates of isolated rat kidney mitochondria. The effect of elevated temperature on the ion suppression was also demonstrated. Graphical Abstract A fragment of overlaid LC retention time-m/z planar views demonstrates the improved separation performance in the analysis of a complex peptide mixture at elevated temperature. Retention time-m/z 2D peptide features detected at 60 °C (magenta) were matched and aligned with features detected at room temperature (green).

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“…The enthalpy became more negative with the retention factors of the analytes and became more negative in each case as the temperature increased. Over a wider range of temperatures a non-linear relationship had observed with the phenyl bonded XTerra column [16].…”

Section: Resultsmentioning

confidence: 99%

“…However, as a method it has been constrained by the poor thermal stability of conventional octadecyl bonded silica based phases but this problem can be overcome by using polymeric materials, such as polystyrene divinylbenzene or alternative thermally stable phases, such as coated zirconia [14] or porous graphitic carbon [13]. More recently the bonded hybrid phases have also shown good stability [15,16]. A repeated concern has been for the thermal stability of the analytes, however, only a few cases of degradation during chromatography have been reported.…”

Section: Introductionmentioning

confidence: 99%