High-temperature liquid chromatography of steroids on a bonded hybrid column

Al-Khateeb, Lateefa A.; Smith, Roger M.

doi:10.1007/s00216-009-2602-6

Cited by 15 publications

(2 citation statements)

References 20 publications

(20 reference statements)

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“…It is known that temperature gradient elution can replace organic solvent gradient elution to improve the chromatographic resolution. [18,21,43] For precise LC/IRMS measurements, the baseline separation of the individual components is required. Therefore, the use of temperature-programmed elution has Figure 5.…”

Section: Temperature-programmed Lc/irms For Csiamentioning

confidence: 99%

High‐temperature reversed‐phase liquid chromatography coupled to isotope ratio mass spectrometry

Zhang

Kujawinski

Jochmann

et al. 2011

Rapid Comm Mass Spectrometry

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Compound-specific isotope analysis (CSIA) by liquid chromatography coupled to isotope ratio mass spectrometry (LC/IRMS) has until now been based on ion-exchange separation. In this work, high-temperature reversed-phase liquid chromatography was coupled to, and for the first time carefully evaluated for, isotope ratio mass spectrometry (HT-LC/IRMS) with four different stationary phases. Under isothermal and temperature gradient conditions, the column bleed of XBridge C(18) (up to 180 °C), Acquity C(18) (up to 200 °C), Triart C(18) (up to 150 °C), and Zirchrom PBD (up to 150 °C) had no influence on the precision and accuracy of δ(13) C measurements, demonstrating the suitability of these columns for HT-LC/IRMS analysis. Increasing the temperature during the LC/IRMS analysis of caffeine on two C(18) columns was observed to result in shortened analysis time. The detection limit of HT-RPLC/IRMS obtained for caffeine was 30 mg L(-1) (corresponding to 12.4 nmol carbon on-column). Temperature-programmed LC/IRMS (i) accomplished complete separation of a mixture of caffeine derivatives and a mixture of phenols and (ii) did not affect the precision and accuracy of δ(13)C measurements compared with flow injection analysis without a column. With temperature-programmed LC/IRMS, some compounds that coelute at room temperature could be baseline resolved and analyzed for their individual δ(13)C values, leading to an important extension of the application range of CSIA.

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Section: Temperature-programmed Lc/irms For Csiamentioning

confidence: 99%

High‐temperature reversed‐phase liquid chromatography coupled to isotope ratio mass spectrometry

Zhang

Kujawinski

Jochmann

et al. 2011

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

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“…Al-Khateeb and Smith [105] studied the use of a hybrid stationary phase, Xterra MS C18 3.5 mm, 50 mm Â 4.6 mm column, in the HTLC analysis of steroids. The effects on retention and efficiency was investigated with variation of temperature and ratio of the methanol:water eluent composition at temperatures up to 130 C. It was found that increasing the temperature enabled the percentage of methanol in the mobile phase to be reduced and even eliminated by use of superheated water at 130 C. The increased temperature on the bonded hybrid column reduced retention times.…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

Developments in Fast Liquid Chromatographic Analysis of Pharmaceuticals

Natishan

2011

Journal of Liquid Chromatography & Related Technologies

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& The need for pharmaceutical analytical laboratories to increase sample throughput and speed of analysis to meet accelerated drug development timelines has led to considerable interest in the development of fast liquid chromatographic technology. Fast liquid chromatographic pharmaceutical analysis is accomplished by developments in ultra-fast high performance liquid chromatographic instrumentation, emerging technologies in column stationary phases and high temperature liquid chromatographic analysis. This review provides an overview and gives recent developments and applications in the different strategies used in fast liquid chromatographic pharmaceutical analysis.

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Subcritical Water Chromatography — An Economical and Green Separation Technique

Yang

Kapalavavi

2011

Encyclopedia of Analytical Chemistry

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Subcritical water chromatography (SBWC) refers to a new reversed‐phase liquid chromatography (RPLC) technique where high‐temperature water is used as the sole mobile phase component. The major advantage of SBWC is the elimination of toxic mobile phase organic solvents required in traditional RPLC. These organic solvents are not only expensive in terms of purchasing price but also costly in their waste disposal. Therefore, the SBWC technique offers both economical and environmental benefits. Additional advantages of SBWC are fast analysis time; temperature‐dependent efficiency, selectivity, and resolution; temperature‐programmed elution; and ability to accommodate both gas‐ and liquid‐phase detectors. Most importantly, after years of academic studies, industry started paying attention to this economical and green separation technique. For example, Procter & Gamble has recently funded an SBWC project in developing green SBWC methods for the analysis of skincare products.

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High-temperature liquid chromatography of steroids on a bonded hybrid column

Cited by 15 publications

References 20 publications

High‐temperature reversed‐phase liquid chromatography coupled to isotope ratio mass spectrometry

High‐temperature reversed‐phase liquid chromatography coupled to isotope ratio mass spectrometry

Developments in Fast Liquid Chromatographic Analysis of Pharmaceuticals

Subcritical Water Chromatography — An Economical and Green Separation Technique

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