Supercritical fluid processing of proteins

Moshashaée, Saeed; Bisrat, Mikael; Forbes, Robert T.; Nyqvist, H; York, Peter

doi:10.1016/s0928-0987(00)00108-1

Cited by 74 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both polymers such as poly( L ‐lactide) (PLLA)24 and proteins such as lysozyme25–29 have been widely studied separately in supercritical CO 2 . In this study, we attempted to prepare lysozyme‐loaded PLLA microparticles using the SEDS process, and poly(ethylene glycol) (PEG) was also used in the process to increase the shelf‐stability and biocompatibility of the protein‐loaded microparticles.…”

Section: Introductionmentioning

confidence: 99%

Study of lysozyme‐polymer composite microparticles in supercritical CO₂

Chen

Yin

et al. 2012

J of Applied Polymer Sci

View full text Add to dashboard Cite

Lysozyme-loaded polymeric composite microparticles were successfully coprecipitated by solution-enhanced dispersion by supercritical CO 2 (SEDS), starting with a homogeneous organic solvent solution of lysozyme/poly(L-lactide)/poly(ethylene glycol) (lysozyme/PLLA/PEG). The effects of different drug loads (5, 8, and 12% w/w), PLLA Mw (10, 50, 100, and 200 kDa), PEG contents (0, 10, 30, and 50% PEG/(PLLAþPEG) w/ w), and PEG Mw (400, 1000, and 4000 kDa) on the surface morphology, particle size, and drug release profile of the resulting composite microparticles were investigated. The results indicate that the size of the microparticles decreased and the rate of drug release increased with an increase in drug load, PEG content, or PEG Mw; the particle size first increased and then decreased with an increase in PLLA Mw, and the drug release was controlled by both particle size and PLLA Mw. The Fourier transform infrared spectrometer analysis and circular dichroism spectra measurement reveal that no significant changes occurred in the molecular structures during the SEDS processing, which is favorable to the production of protein-polymer composite microparticles for a protein drug delivery system.

show abstract

Section: Introductionmentioning

confidence: 99%

Study of lysozyme‐polymer composite microparticles in supercritical CO₂

Chen

Yin

et al. 2012

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…Spray freezedrying may maintain the stability of proteins during the drying of the protein solution into solid particles, since no high temperature is involved [12]. In the process of spray freezedrying, a stream of aqueous solution of protein is first broken into tiny droplets by an ultrasonic atomizer and the resulting droplets are solidified in a continuous low-temperature phase [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Stabilization and encapsulation of human immunoglobulin G into biodegradable microspheres

Wang

Chua

Wang

2004

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

“…Moreover, the conditions are set up so that SC-CO 2 can extract the solvent from the solution at the same time as it meets and disperses the solution. This technique has been used for the processing of diverse materials including low molecular weight substances, proteins and polymers [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Micronization of the officinal component baicalin by SEDS‐PA process

Suo

et al. 2007

Cryst. Res. Technol.

View full text Add to dashboard Cite

Application of micronizing technologies in processing Chinese herbal medicines is very important to improve the forms of prepared Chinese herbal medicines and promote their therapeutic efficacy. Baicalin, a major active component of the typical Chinese herb medicine Scullateria baicallensis Georgi, was micronized using the Solution Enhanced Dispersion by Supercritical fluids though Prefilming Atomization (SEDS-PA) process with the aim of evaluating the efficiency of applying supercritical fluid precipitation technologies in Chinese herb medicine. This study has shown that acicula or rod-like baicalin crystals with Particle Size (PS) of about 20×100 µm were successfully micronized by the SEDS-PA process to long rod-like, twisted fiber-like or fibrous net-like microparticles with PS of 0.1-2.2 µm in width within the range of experiments performed. It was found that a substantial reduction of baicalin microparticles' sizes could lead to a marked increase of adhesions among them and subsequent microparticles agglomeration. With the increase of supercritical CO 2 flow rate and the decrease of solution concentration and solution flow rate, smaller and much more agglomerated microparticles were obtained. Increasing pressure led to formation of smaller microparticles. A larger tendency of particles agglomeration was produced at a higher temperature.

show abstract

Supercritical fluid processing of proteins

Cited by 74 publications

References 13 publications

Study of lysozyme‐polymer composite microparticles in supercritical CO₂

Study of lysozyme‐polymer composite microparticles in supercritical CO₂

Stabilization and encapsulation of human immunoglobulin G into biodegradable microspheres

Micronization of the officinal component baicalin by SEDS‐PA process

Contact Info

Product

Resources

About

Supercritical fluid processing of proteins

Cited by 74 publications

References 13 publications

Study of lysozyme‐polymer composite microparticles in supercritical CO2

Study of lysozyme‐polymer composite microparticles in supercritical CO2

Stabilization and encapsulation of human immunoglobulin G into biodegradable microspheres

Micronization of the officinal component baicalin by SEDS‐PA process

Contact Info

Product

Resources

About

Study of lysozyme‐polymer composite microparticles in supercritical CO₂

Study of lysozyme‐polymer composite microparticles in supercritical CO₂