Supercritical fluid extraction and cleanup procedures for determination of xenobiotics in biological samples

Nam, K.S.; Kapila, Shubhender; Yanders, A. F.; Puri, Ravi K.

doi:10.1016/0045-6535(90)90195-y

Cited by 46 publications

(10 citation statements)

References 4 publications

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“…Accordingly, the average recovery of alachlor analyte extracted by CO2 and 1 mL of methanol at 400-500 atm, increased to 80% (liq CO2 in Figure 4). Overall, the pumpless apparatus showed similar capability and advantages noted in previous studies which utilized a pump for the static SFE of environmental pollutants, such as pesticides, PCBs, and PAHs, etc., from animal tissues (Nam et al, 1990) and soils (Miller et al, 1993).…”

Section: Methodsmentioning

confidence: 99%

Supercritical Fluid Extraction and Enzyme Immunoassay for Pesticide Detection in Meat Products

Nam¹,

King²

1994

J. Agric. Food Chem.

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Section: Methodsmentioning

confidence: 99%

Supercritical Fluid Extraction and Enzyme Immunoassay for Pesticide Detection in Meat Products

Nam¹,

King²

1994

J. Agric. Food Chem.

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“…The advantages and applications of using SFE have been well demonstrated for the analysis of pesticides in food and biological samples. [21][22][23][24][25] Taken with these studies, using the SFE method could reduce sample processing time as well as solvent consumption.…”

mentioning

confidence: 94%

Simultaneous Measurement of Fluoroquinolones in Eggs by a Combination of Supercritical Fluid Extraction and High Pressure Liquid Chromatography

Shim

Lee

Kim

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Simultaneous detection of the fluoroquinolone antibiotics ciprofloxacin, enrofloxacin, ofloxacin, and norfloxacin in eggs by a combination of supercritical fluid extraction (SFE) and high pressure liquid chromatography (HPLC) was studied. Lipid matrices that have been considered to result in poor extraction and isolation of fluoroquinolones in eggs were removed first by SFE with supercritical CO(2) alone, and then the fluoroquinolones were extracted by SFE with supercritical CO(2) containing 20% (v/v) methanol for HPLC analysis. A time-course study of the extraction of lipid matrices of eggs suggested that the SFE method successfully removed the matrices within 20 min. When the fluoroquinolones added to control eggs were extracted by SFE, the extraction efficiency was similar to that by the solvent extraction method, giving the recovery percentages from 83 to 96% in a 40 min-extraction time. The fluoroquinolones extracted from eggs by SFE were analyzed simultaneously by HPLC equipped with a fluorescence detector with detection sensitivity at about 10 ppb for the detection limit. The standard calibration profiles of fluoroquinolones showed linear responses to HPLC, showing more than 0.995 for the mean r(2) value. This is the first report of the simultaneous measurement of fluoroquinolones in eggs by a combination of SFE and HPLC. Using the SFE method allowed us to avoid extensive sample preparation such as solvent extraction and chromatographic cleanup that are basically required in extraction of fluoroquinolones.

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“…However, lyophilization of the tissues prior to extraction conserves considerable vessel volume by eliminating the need for inclusion of drying agents, such as sodium sulfate or diatomaceous earth. These reagents have been used by a number of researchers to immobilize moisture in the samples and to obtain better extraction efficiencies (17,18,20). Water content of the fishes used in our experiments ranged from 75% to 85% of the fresh weight of the tissue.…”

Section: Discussionmentioning

confidence: 99%

Determination of PCBs in Fish Tissues Using Supercritical Fluid Extraction

Hale¹,

Gaylor²

1995

Environ. Sci. Technol.

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Supercritical fluid extraction and cleanup procedures for determination of xenobiotics in biological samples

Cited by 46 publications

References 4 publications

Supercritical Fluid Extraction and Enzyme Immunoassay for Pesticide Detection in Meat Products

Supercritical Fluid Extraction and Enzyme Immunoassay for Pesticide Detection in Meat Products

Simultaneous Measurement of Fluoroquinolones in Eggs by a Combination of Supercritical Fluid Extraction and High Pressure Liquid Chromatography

Determination of PCBs in Fish Tissues Using Supercritical Fluid Extraction

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