Super-resolution fluorescence imaging of chromosomal DNA

Zessin, Patrick J. M.; Finan, Kieran; Heilemann, Mike

doi:10.1016/j.jsb.2011.12.015

Cited by 103 publications

(103 citation statements)

References 41 publications

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“…Zessin and colleagues are the¯rst to successfully combine 5-Ethynyl-20-deoxyuridine (EdU) labeling and dSTORM technology for super-resolution imaging of DNA. 60 Although these emerging probes can improve the imaging quality of SMLM in some aspects, the category of biomolecules labeled by these strategies is limited and impedes their wide applications in super-resolution imaging.…”

Section: Emerging Small Molecular Labeling Methodsmentioning

confidence: 99%

Super-resolution imaging in glycoscience: New developments and challenges

Chen

Tong

Wang

2016

J. Innov. Opt. Health Sci.

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Carbohydrates on cell surfaces play a crucial role in a wide variety of biological processes, including cell adhesion, recognition and signaling, viral and bacterial infection, in°ammation and metastasis. However, owing to the large diversity and complexity of carbohydrate structure and nongenetically synthesis, glycoscience is the least understood¯eld compared with genomics and proteomics. Although the structures and functions of carbohydrates have been investigated by various conventional analysis methods, the distribution and role of carbohydrates in cell membranes remain elusive. This review focuses on the developments and challenges of super-resolution imaging in glycoscience through introduction of imaging principle and the available°uorescent probes for super-resolution imaging, the labeling strategies of carbohydrates, and the recent applications of super-resolution imaging in glycoscience, which will promote the super-resolution imaging technology as a promising tool to provide new insights into the study of glycoscience.

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Section: Emerging Small Molecular Labeling Methodsmentioning

confidence: 99%

Super-resolution imaging in glycoscience: New developments and challenges

Chen

Tong

Wang

2016

J. Innov. Opt. Health Sci.

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“…This so-called bio-orthogonal 'click chemistry' has led to an explosion of interest in the selective covalent labeling of molecules in cells and living organisms (Kolb et al, 2001;Prescher and Bertozzi, 2005;Zessin et al, 2012). Click chemistry relies on a copper-catalyzed azide-alkyne cycloaddition (Box 1).…”

Section: Box 2 Reference Structures For Super-resolution Imagingmentioning

confidence: 99%

“…As a logical consequence, photoactivated localization microscopy (PALM) (Betzig et al, 2006;Shroff et al, 2007;Shroff et al, 2008), fluorescence photoactivation localization microscopy (FPALM) Hess et al, 2007) and stochastic optical reconstruction microscopy (STORM) using special pairs of fluorophores (Rust et al, 2006;Bates et al, 2007) were introduced shortly afterwards. A short time later, the use of commercially available organic fluorescent probes, such as fluorophore-labeled antibodies or phalloidin probes, was demonstrated for super-resolution imaging by direct STORM (dSTORM), facilitating the acceptance and broad applicability of localization microscopy Heilemann et al, 2009;van de Linde et al, 2011a;Jones et al, 2011;Dempsey et al, 2011;Sillibourne et al, 2011;Williamson et al, 2011;Kaminski-Schierle et al, 2011;van de Linde et al, 2012;Zessin et al, 2012;Lampe et al, 2012;Wilmes et al, 2012;Rossy et al, 2013;Mattila et al, 2013). Furthermore, other localization microscopy methods using photoswitching of standard organic fluorophores under slightly different experimental conditions emerged under the names of GSDIM (ground-state depletion microscopy followed by individual molecule return) (Fölling et al, 2008) and blink microscopy .…”

Section: Introductionmentioning

confidence: 99%

Localization microscopy coming of age: from concepts to biological impact

Sauer

2013

Journal of Cell Science

105

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SummarySuper-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging techniques introduced in recent years, localization microscopy excels because it delivers single-molecule information about molecular distributions, even giving absolute numbers of proteins present in subcellular compartments. This provides insight into biological systems at a molecular level that can yield direct experimental feedback for modeling the complexity of biological interactions. In addition, efficient new labeling methods and strategies to improve localization are emerging that promise to achieve true molecular resolution. This raises localization microscopy as a powerful complementary method for correlative light and electron microscopy experiments.

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“…Stochastic LM imaging on isolated DNA has been demonstrated using DNA intercalating dyes [180,181], but this methodology still needs to be refined in order to be used in whole cells [182]. Recently, the Heilemann lab has reported on a click chemistry reaction for DNA labelling that allowed bright photoswitchable carbocyanine (Alexa 647) fluorophores to be introduced into DNA at a density compatible with dSTORM imaging at 20 nm resolution [183]. Chromosomal organisation in bacteria using super-resolution imaging has been studied with nucleoid-associated tagged proteins (HU, H-NS), revealing their distribution in clusters at different stages of the cell cycle [184] and hinting at a key role in global chromosomal organisation [185].…”

Section: Chromosome Organisationmentioning

confidence: 99%

Fluorescence nanoscopy. Methods and applications

Requejo-Isidro

2013

J Chem Biol

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Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffractionlimited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.

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Super-resolution fluorescence imaging of chromosomal DNA

Cited by 103 publications

References 41 publications

Super-resolution imaging in glycoscience: New developments and challenges

Super-resolution imaging in glycoscience: New developments and challenges

Localization microscopy coming of age: from concepts to biological impact

Fluorescence nanoscopy. Methods and applications

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