2013
DOI: 10.1007/s12154-013-0096-3
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Fluorescence nanoscopy. Methods and applications

Abstract: Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffractionlimited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.

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Cited by 34 publications
(22 citation statements)
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References 217 publications
(285 reference statements)
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“…Most importantly,the intensity of the C-Naphox fluorescence signal remained virtually unchanged, even after recording five images (Figure 3a), whereas as ignificant decrease in signal intensity was detected for cells labelled with Alexa 488 during the measurement (Figure 3b). [17] Thepolarity-responsive fluorescence with large Stokes shifts should impart C-Naphox with the potential for multi-color imaging and thus increase its value even further. To our knowledge,C -Naphox is thus the first fluorescent dye that permits repeated STED imaging at such al ow degree of photodegradation.…”
Section: Methodsmentioning
confidence: 99%
“…Most importantly,the intensity of the C-Naphox fluorescence signal remained virtually unchanged, even after recording five images (Figure 3a), whereas as ignificant decrease in signal intensity was detected for cells labelled with Alexa 488 during the measurement (Figure 3b). [17] Thepolarity-responsive fluorescence with large Stokes shifts should impart C-Naphox with the potential for multi-color imaging and thus increase its value even further. To our knowledge,C -Naphox is thus the first fluorescent dye that permits repeated STED imaging at such al ow degree of photodegradation.…”
Section: Methodsmentioning
confidence: 99%
“…Thee nhancement of the fluorophore stability under STED imaging conditions is absolutely imperative for the practical application of STED imaging in the visualization of various biological events.The remarkable photostability of C-Naphox should thus open ap reviously unattainable avenue to record prolonged live STED imaging, potentially enabling the visualization of various biological events with high spatial resolution. [17] Thepolarity-responsive fluorescence with large Stokes shifts should impart C-Naphox with the potential for multi-color imaging and thus increase its value even further.…”
Section: Methodsmentioning
confidence: 99%
“…Finally, it is interesting to consider that modulation of FP emission is the core concept of localisation-based 'superresolution' light microscopy techniques including PALM and STORM [27]. It would be a logical step to investigate Fig.…”
Section: -25]mentioning
confidence: 99%