Super-High-Throughput Screening of Enantioselective Catalysts by Using Capillary Array Electrophoresis

Reetz, Manfred T.; Kühling, Klaus M.; Deege, Alfred; Hinrichs, Heike; Belder, Detlev

doi:10.1002/1521-3773(20001103)39:21<3891::aid-anie3891>3.0.co;2-1

Cited by 212 publications

(89 citation statements)

References 48 publications

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“…This is especially useful as the limited available separation length is often considered to be a limiting factor for an intended MCE separation. Especially for demanding separations like chiral analysis on microchips [7] it has been reported that the separation length has a significant impact on resolution, therefore folded microchannels have been used to enable chiral separations on chips [42,43]. However, the need to incorporate turns, in order to realize long channels on a compact microchip, adds a geometrical contribution to analyte dispersion [44][45][46].…”

Section: Resultsmentioning

confidence: 99%

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Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

et al. 2002

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The channels of microfluidic glass chips have been coated with poly(vinyl alcohol) (PVA). Applied for microchip electrophoresis, the coated devices exhibited a suppressed electroosmotic flow and improved separation performance. The superior performance of PVA-coated channels could be demonstrated by electrophoretic separations of labeled amines and by video microscopy. While a distorted sample zone is injected using uncoated channels the application of PVA-coated channels results in an improved shape of the sample zone with less band broadening. Applying PVA-coated microchips for the separation of amines labeled with Alexa Fluor 350 even sub-second separations, utilizing a separation length of only 650 microm, could be obtained, while this was not possible using uncoated devices. By using PVA-coated devices rather than an uncoated chip a threefold increase in separation efficiencies could be observed. As the electroosmotic flow (EOF) was suppressed, the anionic compounds were detected at the anode whereas the dominant EOF in uncoated devices resulted in an effective mobility to the cathode. Besides improved separation performance another important feature of the PVA-coated channels was the suppressed adsorption of fluorescent compounds in repetitive runs which results in an improved robustness and detection sensitivity. Applying PVA-coated channels, rinsing or etching steps could be omitted while this was necessary for a reliable operation of uncoated devices.

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Section: Resultsmentioning

confidence: 99%

“…Microfluidic devices have been applied for electrophoretic separations for a variety of biochemical and chemical analysis [1][2][3][4][5][6][7]. Latest developments in microchip electrophoresis (MCE) have been summarized in recent reviews [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

et al. 2002

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“…To date, a number of techniques are under development for the high-throughput enantiomeric composition determination [13][14][15][16][17], including time resolved IR-thermographic method [18][19][20][21], electron spray mass spectrometry [22][23][24][25][26], capillary electrophoresis [27], UV/Vis [28][29][30], circular dichroism [31,32], and fluorescence [33,34]. Among these approaches, optical methods have been attracting increasing attention due to their ability for quick data collection and compatibility with high-throughput screening system.…”

Section: Advances In Chemistrymentioning

confidence: 99%

Chiral Recognition by Fluorescence: One Measurement for Two Parameters

Sheng

2014

Advances in Chemistry

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This outlook describes two strategies to simultaneously determine the enantiomeric composition and concentration of a chiral substrate by a single fluorescent measurement. One strategy utilizes a pseudoenantiomeric sensor pair that is composed of a 1,1 -bi-2-naphthol-based amino alcohol and a partially hydrogenated 1,1 -bi-2-naphthol-based amino alcohol. These two molecules have the opposite chiral configuration with fluorescent enhancement at two different emitting wavelengths when treated with the enantiomers of mandelic acid. Using the sum and difference of the fluorescent intensity at the two wavelengths allows simultaneous determination of both concentration and enantiomeric composition of the chiral acid. The other strategy employs a 1,1 -bi-2-naphthol-based trifluoromethyl ketone that exhibits fluorescent enhancement at two emission wavelengths upon interaction with a chiral diamine. One emission responds mostly to the concentration of the chiral diamine and the ratio of the two emissions depends on the chiral configuration of the enantiomer but independent of the concentration, allowing both the concentration and enantiomeric composition of the chiral diamine to be simultaneously determined. These strategies would significantly simplify the practical application of the enantioselective fluorescent sensors in high-throughput chiral assay.

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Section: Introductionmentioning

confidence: 99%

Surface modification in microchip electrophoresis

2003

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Different approaches and techniques for surface modification of microfluidic devices applied for microchip electrophoresis are reviewed. The main focus is on the improved electrophoretic separation by reducing analyte-wall interactions and manipulation of electroosmosis. Approaches and methods for permanent and dynamic surface modification of microfluidic devices, manufactured from glass, quartz and also different polymeric substrates, are described.

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Super-High-Throughput Screening of Enantioselective Catalysts by Using Capillary Array Electrophoresis

Cited by 212 publications

References 48 publications

Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

Chiral Recognition by Fluorescence: One Measurement for Two Parameters

Surface modification in microchip electrophoresis

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