Abstract:The antisialagogic effect of imipramine (IMP) on sympathetic and parasympathetic salivation was demonstrated in mice chronically administered with IMP. The measurement of phenylephrine (20mg/kg, s.c.) and pilocarpine (0.8mg/kg, s.c.)-induced salivation was carried out by a modified Richter's method which measures the amount of saliva secreted by the size of the spots of mouse saliva stained on a filter paper.IMP (32mg/kg, s.c.) was administered once a day for 21 successive days. The results obtained were summarized as follows. 1) IMP (16-64mg/kg, s.c.), administered singly, inhibited both phenylephrine and pilocarpine-induced salivation depending on the dosage, but did not completely inhibit salivation.2) The antisialagogic effect of IMP on salivation was inhibited by the chronic administration.3) The inhibitory effect of IMP was detected not only on the 7 th day after the beginning of administration, but also 14 or 21 days after. From these results, it is suggested that pharmacological denervationsupersensitivity on sympathetic and parasympathetic salivation in mice salivary glands is developed byrepeated administration of IMP.