<sup>13</sup>C CP/MAS NMR Spectroscopy in the Analysis of Pectins

Sinitsya, A.; Čopı́ková, Jana; Pavlíková, Helena

doi:10.1080/07328309808002328

Cited by 41 publications

(11 citation statements)

References 15 publications

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“…Previous work showed these signal shifts at δ 100 and 99.3 [ 1 ] and δ 100.1 and 99.4 [ 19 ], respectively. This is also similar to earlier reports of signals of C-1 of low methoxy pectins in the range δ 101.03–101.43 [ 44 ] and of anomeric carbon of α -linkage [ 45 ]. Signals observed in the region, δ 170.78, 173.95, 179.87, and 185.44, were assigned to carbonyl groups of the esterified and nonesterified units [ 46 , 47 ].…”

Section: Resultssupporting

confidence: 92%

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Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical

Adi-Dako

Ofori-Kwakye

Manso

et al. 2016

Journal of Pharmaceutics

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The physicochemical and antimicrobial properties of cocoa pod husk (CPH) pectin intended as a versatile pharmaceutical excipient and nutraceutical were studied. Properties investigated include pH, moisture content, ash values, swelling index, viscosity, degree of esterification (DE), flow properties, SEM, FTIR, NMR, and elemental content. Antimicrobial screening and determination of MICs against test microorganisms were undertaken using agar diffusion and broth dilution methods, respectively. CPH pectin had a DE of 26.8% and exhibited good physicochemical properties. Pectin had good microbiological quality and exhibited pseudoplastic, shear thinning behaviour, and high swelling capacity in aqueous media. The DE, FTIR, and NMR results were similar to those of previous studies and supported highly acetylated low methoxy pectin. CPH pectin was found to be a rich source of minerals and has potential as a nutraceutical. Pectin showed dose-dependent moderate activity against gram positive and gram negative microorganisms but weak activity against Listeria spp. and A. niger. The MICs of pectin ranged from 0.5 to 4.0 mg/mL, with the highest activity against E. coli and S. aureus (MIC: 0.5–1.0 mg/mL) and the lowest activity against A. niger (MIC: 2.0–4.0 mg/mL). The study has demonstrated that CPH pectin possesses the requisite properties for use as a nutraceutical and functional pharmaceutical excipient.

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Section: Resultssupporting

confidence: 92%

“…Previous report showed similar high frequency C-6 signals at δ 170.6 and 173.4, respectively [ 19 ]. Signals at δ 17.59 were attributed to methyl carbons of rhamnose residues [ 44 ]. Signals attributed to C-2, 3, 4, and 5 in the galacturonic acid units were found in the range δ 66.76–80.59.…”

Section: Resultsmentioning

confidence: 99%

Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical

Adi-Dako

Ofori-Kwakye

Manso

et al. 2016

Journal of Pharmaceutics

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“…The region of 190-160 ppm of 13 C CP/ MAS NMR spectrum belongs to the carboxyl C-6 carbons of galacturonic units that are present as carboxylic acid COOH, carboxylate anion COO À , or ester COOCH 3 . 25 The content of polygalacturonic acid units (PGA), DM and DAc of pectin samples were evaluated as the ratio of integral intensity of the C-6, COOCH 3 and O-COCH 3 carbons, respectively. Jarvis and apperley measured 13 C CP/MAS NMR spectra of solid pectin acid, sodium pectate and the acid form of pectin, and they reported that the carboxyl resonance was at 171.6 ppm for the protonated and esteried forms and 176 ppm for the ionized form.…”

Section: Structure Analysis Of Pectin In Tobacco Samplesmentioning

confidence: 99%

“…It has been reported that the pectins extracted with bacterial enzymes had a higher O-acetyl content than those extracted with acid. 5,25 Note that highly acetylated pectins have bad gelation properties and must be deacetylated by chemical or enzymatic processing. 1 Thus, solid-state NMR may be a good method to monitor the values of DAc in the process of extraction or acetyl hydrolysis.…”

Section: Structure Analysis Of Pectin In Tobacco Samplesmentioning

confidence: 99%

Quantitative and structure analysis of pectin in tobacco by¹³C CP/MAS NMR spectroscopy

et al. 2014

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“…2). The signals were assigned according to Saulnier et al (1988), Westerlund et al (1990), Colquhoun et al (1990), Sinitsya et al (1998) (see Tables 3 and 4). The peaks can be assigned to arabinan, galactan and rhamnogalacturonan.…”

Section: Solid-state Cp/mas 13 C-nmrmentioning

confidence: 99%

Celery (Apium graveolens) parenchyma cell walls: cell walls with minimal xyloglucan

Thimm

Burritt

Sims³

et al. 2002

Physiologia Plantarum

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The primary walls of celery (Apium graveolens L.) parenchyma cells were isolated and their polysaccharide components characterized by glycosyl linkage analysis, cross-polarization magic-angle spinning solid-state 13C nuclear magnetic resonance (CP/MAS 13C NMR) and X-ray diffraction. Glycosyl linkage analysis showed that the cell walls consisted of mainly cellulose (43 mol%) and pectic polysaccharides (51 mol%), comprising rhamnogalacturonan (28 mol%), arabinan (12 mol%) and galactan (11 mol%). The amounts of xyloglucan (2 mol%) and xylan (2 mol%) detected in the cell walls were strikingly low. The small amount of xyloglucan present means that it cannot coat the cellulose microfibrils. Solid-state 13C NMR signals were consistent with the constituents identified by glycosyl linkage analysis and allowed the walls to be divided into three domains, based on the rigidity of the polymers. Cellulose (rigid) and rhamnogalacturonan (semi-mobile) polymers responded to the CP/MAS 13C NMR pulse sequence and were distinguished by differences in proton spin relaxation time constants. The arabinans, the most mobile polymers, responded to single-pulse excitation (SPE), but not CP/MAS 13C NMR. From solid-state 13C NMR of the cell walls the diameter of the crystalline cellulose microfibrils was determined to be approximately 3 nm while X-ray diffraction of the cell walls gave a value for the diameter of approximately 2 nm.

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¹³C CP/MAS NMR Spectroscopy in the Analysis of Pectins

Cited by 41 publications

References 15 publications

Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical

Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical

Quantitative and structure analysis of pectin in tobacco by¹³C CP/MAS NMR spectroscopy

Celery (Apium graveolens) parenchyma cell walls: cell walls with minimal xyloglucan

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13C CP/MAS NMR Spectroscopy in the Analysis of Pectins

Cited by 41 publications

References 15 publications

Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical

Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical

Quantitative and structure analysis of pectin in tobacco by13C CP/MAS NMR spectroscopy

Celery (Apium graveolens) parenchyma cell walls: cell walls with minimal xyloglucan

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Product

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About

¹³C CP/MAS NMR Spectroscopy in the Analysis of Pectins

Quantitative and structure analysis of pectin in tobacco by¹³C CP/MAS NMR spectroscopy