Quantitative and structure analysis of pectin in tobacco by¹³C CP/MAS NMR spectroscopy

Abstract

A new method utilizing13C CP/MAS NMR spectra was developed for the simultaneous quantitative and structure analysis of pectin in tobacco. The method was employed to measure pectin after the extraction of tobacco samples with acid under optimal conditions (pH 2.0 for 1.5 h at 85 °C).

Development of a rapid method for the quantification of cellulose in tobacco by 13 C CP/MAS NMR

“…Despite the same instrument parameter settings, some spectra have high noise level than others of the same number of samples, due to the instrument volatility and the inhomogeneity of the samples (Alesiani et al, 2005). The 13 C CP/MAS NMR signal of some 13 C nuclei may be lost or decreased somewhat unobservable (Zhu, Liu, Zheng, & Gao, 2014). Differential loss of signal could cause distortion of the signals of the carbon in the samples.…”

“…Conventional method for determining the cellulose content in plant is chemical method that involves tedious fractionation or extraction procedures (Bokelman et al, 1983). The potential of 13 C CP/MAS NMR for the nondestructive analysis of plants has attracted widespread concern and extensive researches (Kao et al, 2008;Paoli, Bighelli, Castola, Tomi, & Casanova, 2013;Sievers et al, 2009;Wickholm, Hult, Larsson, Iversen, & Lennholm, 2001;Zhu et al, 2014). Importantly, one challenge to studying polymorphic mixtures using 13 C CP/MAS NMR spectroscopy is the interference of overlapping peaks.…”

“…Importantly, one challenge to studying polymorphic mixtures using 13 C CP/MAS NMR spectroscopy is the interference of overlapping peaks. The broad resonance band of C-1 region (95-115 ppm) arises from a superposition of several individual peaks, pectin respectively (Zhu et al, 2014). Overlapping peaks occupy an indispensable proportion in the C-1 region even rough in the spectrum of detergent pretreatment samples, and must be taken into consideration.…”

Carbohydrates—Key Players in Tobacco Aroma Formation and Quality Determination

“…Pectin content is important for tobacco quality determination. With increasing pectin content, tobacco blend becomes soft and sensitive at high air humidity, or rigid and fragile at low air humidity [34]. However, pectin is considered as an undesirable compound in tobacco product because of poor smoking characteristics [35].…”

Solid-State 77Se NMR of Organoselenium Compounds through Cross Polarization Magic Angle Spinning (CPMAS) Method

“…A method by using a combination of cross-polarization (CP) and MAS was applied, which has been previously used for the characterization of 13 C, 31 P etc 39–43 . The method offers a beneficial solution to achieve higher SNR based on the applications of polarization transfer from the abundant proton ( 1 H) spin to low abundance nuclei i.e.…”