Sunflower seed husk agar: A new medium for the differentiation of Candida dubliniensis from Candida albicans

Background and Objectives:Patients with diabetes mellitus are prone to secondary infections. In this study we aim to determine the prevalence of one such secondary infection (oral Candida colonization) and evaluate the influence of local and systemic factors on the oral candidal colonization in patients with diabetes mellitus.Materials and Methods:Forty non-insulin-dependent diabetic patients and 40 healthy individuals were included in this study. Samples were collected by using the oral rinse method. The candidal species were isolated and identified through phenotypic methods. An in vitro antifungal susceptibility profile was evaluated. Glycemic control, as determined by the glycosylated hemoglobin concentrations (HbA1c) of the study subjects, was correlated with the candidal colonization.Results:Patients with diabetes showed a significantly higher prevalence of candidal colonization. The rate of carriage and density (P = 0.001) was higher. Candida albicans was the most predominantly isolated species, however, C. dubliniensis, C. tropicalis, and C. parapsilosis were also observed. Variable resistance toward the antifungal drugs (amphotericin B and fluconazole) was observed in the Candida isolated from diabetics, but not from healthy patients. Interestingly, a positive correlation was observed between glycemic control and candidal colonization.Conclusion:Diabetic patients had a higher candidal carriage rate, with a variety of candidal strains, which significantly varied in their resistance to routinely used anti-fungal agents. Interestingly the higher oral candidal colonization in diabetic patients is related to local and systemic factors, independent of their oral habits.

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“…An additional test for growth at 45°C was used to discriminate between C. albicans and C. dubliniensis . [20]…”

Section: Methodsmentioning

confidence: 99%

Detection of species diversity in oral candida colonization and anti-fungal susceptibility among non-oral habit adult diabetic patients

Premkumar¹,

Ramani²,

Chandrasekar³

et al. 2014

J Nat Sc Biol Med

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“…Comparing this method with other phenotypic identification methods such as incubation at different temperatures and growth in different culture media as done previously in our laboratory [21,22] as well as other laboratories [23] will also help to determine the best possible easy-to-perform, early identification method to differentiate these two Candida species.…”

Section: Discussionmentioning

confidence: 94%

Rapid Differentiation of Candida dubliniensis from Candida albicans by Early D-Xylose Assimilation

Ellepola

Khan

2012

Med Princ Pract

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Objective: To determine if D-xylose (XYL) and/or α-methyl-D-glucoside (MDG) assimilation can be used reliably as a rapid test to differentiate Candida dubliniensis from Candida albicans at an earlier time point such as 2 h after inoculation. Materials and Methods: Thirty isolates of C. albicans and C. dubliniensis recovered from anatomical sites and clinical specimens were used. Isolates were inoculated into the API 20C AUX yeast identification system, and incubated at 30°C. XYL and MDG assimilations were read at 2-hour intervals beginning 2 h after the initial inoculation and up to 24 h of incubation; thereafter, results were read after 48 and 72 h. Results: Twenty-nine (97%) C. albicans isolates had assimilated XYL at 16 h and, by 24 h, all isolates were positive for XYL assimilation. None of the C. dubliniensis isolates assimilated XYL. The MDG assimilation revealed that 24, 40, 92 and 100% of C. albicans isolates became positive after 16, 24, 48 and 72 h of incubation, respectively, whereas only 3% of C. dubliniensis isolates assimilated MDG after 72 h. Conclusions: The findings showed that it is possible to rapidly differentiate C. albicans from C. dubliniensis isolates using the API 20C AUX carbohydrate assimilation kits after 16 h of incubation at 30°C based on the XYL assimilation.

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“…albicans and C. dubliniensis are phylogenetically related species and share several phenotypic characteristics, often leading to misdiagnosis. The use of several phenotypic techniques has been proposed by different authors to accurately identify these species [10,17,18]. However, in many cases, the interpretation of these tests also relies on subjective descriptions, such as changes in color tones on standard chromogenic media or growth on hypertonic media [19].…”

Section: Discussionmentioning

confidence: 99%

“…The strains were streaked, as described by Khan et al [17], incubated at 28°C, for three days, and daily observed to evaluate the texture of the colonies. On this agar, C. albicans colonies have a smooth texture, while those of C. dubliniensis have a rough texture [17].…”

Section: Growth On Sunflower Seed Agarmentioning

confidence: 99%

Combination of Phenotypic Tests as a Screening Approach for the Differentiation of Cryptic Species Candida albicans and Candida dubliniensis

Rocha¹,

Bandeira²,

Alencar³

et al. 2017

Med Mycol Open Access

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Sunflower seed husk agar: A new medium for the differentiation of Candida dubliniensis from Candida albicans

Cited by 16 publications

References 19 publications

Detection of species diversity in oral candida colonization and anti-fungal susceptibility among non-oral habit adult diabetic patients

Detection of species diversity in oral candida colonization and anti-fungal susceptibility among non-oral habit adult diabetic patients

Rapid Differentiation of <b><i>Candida dubliniensis</i></b> from <b><i>Candida albicans</i></b> by Early <i>D</i>-Xylose Assimilation

Combination of Phenotypic Tests as a Screening Approach for the Differentiation of Cryptic Species Candida albicans and Candida dubliniensis

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