Sun protection factor, total phenol, flavonoid contents and antioxidant activity of medicinal plants from Iran

Hashemi, Zahra; Ebrahimzadeh, Mohammad Ali; Khalili, Masoumeh

doi:10.4314/tjpr.v18i7.11

Cited by 34 publications

(43 citation statements)

References 15 publications

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“…The total flavonoid content in the methanolic fruit extract of C. prophetarum extract was calculated using quercetin as a standard and was found to be 212.87 mg quercetin/g weight. Previously, the phenolic content in the methanol extract of Cucumis melo fruits was found to be 80 mg gallic acid/g weight, 18 and the flavonoid content in the leaf of the Cucumis africanus was found to be 228.9 mg quercetin/g weight. 19 We found a high flavonoid concentration as compared to phenols.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

2021

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In recent decades, biodegradable polymeric nanoparticles have been used as a nanocarrier for the delivery of anticancer drugs. In the present study, we synthesize bovine serum albumin (BSA) nanospheres and evaluate their ability to incorporate a plant extract with anticancer activity. The plant extract used was the methanol fruit extract of Cucumis prophetarum , which is a medicinal herb. The fruit-extract-encapsulated BSA nanospheres (Cp-BSA nanospheres) were prepared using a desolvation method at various pH values of 5, 7, and 9. The nanosphere formulations were characterized using various techniques such as dynamic light scattering (DLS), ζ-potential, Fourier transform infrared spectroscopy (FTIR), and field-effect scanning electron microscopy (FESEM). The results show that the Cp-BSA nanospheres prepared at pH 7 were spherical with a uniform particle size, low polydispersity index (PDI), ζ-potential, and high entrapment efficiency (82.3%) and showed sustained release of fruit extract from Cp-BSA nanospheres in phosphate-buffered saline (PBS), pH 5. The anticancer activity was evaluated on A549, HepG2, MCF-7 cancer cell lines and HEK 293 normal cell lines. In vitro, antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, intracellular reactive oxygen species (ROS) production, and mitochondrial membrane potential were estimated. An in vitro cellular uptake study was performed using fluorescein isothiocyanate (FITC) dye at a different time of incubation, and DNA fragmentation was observed in a dose-dependent manner. The gene expression level of Bax and the suppression level of Bcl-2 were observed upon the treatment of Cp-BSA nanospheres. Thus, the Cp-BSA nanospheres triggered ROS-dependent mitochondrial apoptosis in different human cancer cell lines when compared to the noncancerous cell lines and could be used as a potential candidate for anticancer agents.

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Section: ■ Results and Discussionmentioning

confidence: 99%

ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

2021

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“…The SPF value of isolate FDi at a concentration of 100 ppm has a value of 2.25287, so it is categorized as a sunscreen with minimal protection. Hashemi et al (2019) have determined the content of flavonoid compounds, antioxidant activity, and sunscreen tests on leaf extracts of several plants. Leaf extracts with high content of flavonoid compounds tend to be directly proportional to antioxidant activity, but the SPF value is not always directly proportional to antioxidant activity.…”

Section: Determination Of Spf Valuementioning

confidence: 99%

Flavonoid Compounds of the Catechin from Wungu (Graptophyllum pictum (L.) Griff) Leaves and the Sun Protecting Factor Value

2022

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The flavonoid content in wungu (Graptophyllum pictum (L.) Griff.) leaves has the potential as a sunscreen. The study aims to identify isolates of flavonoid compounds from wungu leaves and determine the SPF value. Steps are followed by extraction, fractionation, phytochemical test, separation by chromatography, identification, and SPF test. Extraction was carried out with methanol, followed by fractionation with n-hexane and dichloromethane. Dichloromethane fraction was chosen to proceed to the separation step because the results of the phytochemical test showed a vigorous color intensity for the content of flavonoids. The isolate (3.6 mg; dark green; amorphous; mp. 132-136 °C) was identified using a UV-Vis spectrophotometer using methanol as a solvent with a shift reagent NaOH, AlCl3, and a mixture of concentrated AlCl3 and HCl. Based on the UV-Vis spectra, the isolate was predicted to be flavonoid compounds belonging to the catechin group, which have a hydroxyl group at positions C-3, C-7, and do not have an ortho-hydroxy group in ring B. The SPF value of the isolate of 2.3244 at 100 ppm was determined in vitro and calculated by the Qian equation. Therefore, isolate was categorized as sunscreens that provided minimal protection

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“…Human can experience skin damages caused by air pollution as well as long-term direct sunlight exposure (UV radiation) (Jesumani et al, 2020;Hashemi et al, 2019). The skin damages may include sunburn, early aging, inflammation, and both melanoma and non-melanoma skin cancer (Lukman et al, 2015;Ebrahimzadeh et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Isolation of bioactive compounds with tyrosinase inhibitory activity from the methanol extract of meniran herb (Phyllanthus niruri Linn.)

Salsabila¹,

Hartati²,

Muqarrabun³

et al. 2022

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Meniran (Phyllanthus niruri L.) is a wild plant in the Euphorbiaceae family which grows in tropical climates. The plant is known to possess antioxidant activity and is rich in phenolic compounds. Since plants with high phenolic content and high antioxidant activity are known to exhibit high tyrosinase inhibitory activity, the purpose of this study is to determine the inhibitory activity of the tyrosinase enzyme by the methanol extract of meniran (P. niruri L.) and to isolate secondary metabolites from the extract and fractions which exhibit inhibitory activity against tyrosinase. The in vitro tyrosinase inhibitory activity assay was conducted using the 96-well microplate method with kojic acid as the positive control, while the separation and purification of compounds were carried out using chromatography techniques. The methanol extract and n-hexane fraction showed tyrosinase inhibitory activity with IC50 of 11.05±0.16 and 2.57±0.08 mg/ml, respectively. Three bioactive compounds were succesfully isolated from the n-hexane fraction. Based on the UV and NMR spectra, compounds 1, 2, and 3 were identified as phyllanthin, phyltetralin, and hypophyllanthin, respectively. Furthermore, phyllanthin (1) was shown to demonstrate inhibitory activity against tyrosinase with IC50 of 0.6322 mM. In conclusion, both meniran extract and phyllanthin (1) were found to exhibit inhibitory acitivity against the tyrosinase enzyme. In the future they can be candidates for the treatment against hyperpigmentation and also as an active ingredient in skincare formulations.

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Sun protection factor, total phenol, flavonoid contents and antioxidant activity of medicinal plants from Iran

Cited by 34 publications

References 15 publications

ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

Flavonoid Compounds of the Catechin from Wungu (Graptophyllum pictum (L.) Griff) Leaves and the Sun Protecting Factor Value

Isolation of bioactive compounds with tyrosinase inhibitory activity from the methanol extract of meniran herb (Phyllanthus niruri Linn.)

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