2018
DOI: 10.18632/aging.101547
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Abstract: Progressive deterioration of antioxidant response in aging is a major culprit in the initiation of age-related pathobiology induced by oxidative stress. We previously reported that oxidative stress leads to a marked reduction in transcription factor Sp1 and its mediated Prdx6 expression in lens epithelial cells (LECs) leading to cell death. Herein, we examined how Sp1 activity goes awry during oxidative stress/aging, and whether it is remediable. We found that Sp1 is hyper-Sumoylated at lysine (K) 16 residue i… Show more

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Cited by 18 publications
(29 citation statements)
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References 100 publications
(202 reference statements)
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“…Intracellular ROS level was measured by use of fluorescent dye dichlorofluorescin diacetate (H2DCFDA), a nonpolar compound that is converted into a polar derivative (dichlorofluorescein) by cellular esterase after incorporation into cells [7,52,72,73]. On the day of the experiment, the medium was replaced with Hank′s solution containing 10 µM H2DCFDA dye and cells were incubated at 37 °C.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Intracellular ROS level was measured by use of fluorescent dye dichlorofluorescin diacetate (H2DCFDA), a nonpolar compound that is converted into a polar derivative (dichlorofluorescein) by cellular esterase after incorporation into cells [7,52,72,73]. On the day of the experiment, the medium was replaced with Hank′s solution containing 10 µM H2DCFDA dye and cells were incubated at 37 °C.…”
Section: Methodsmentioning
confidence: 99%
“…Prdx6 is a cytosolic protein and is abundantly expressed in lung, eye lens, keratinocytes, skin and brain [43,44,45,46]. It is also localized in ROS-producing organelles, such as endoplasmic reticulum, plasma membrane, lysosomes, mitochondria and cerebrospinal fluid [47,48,49,50,51], suggesting its importance in controlling redox-homeostasis for cell survival [1,48,52,53,54]. Prdx6 protects many cell types from lipid peroxidation-mediated damage to membrane, DNA and protein [1,6,7,49,52,55,56,57,58].…”
Section: Introductionmentioning
confidence: 99%
“…Prdx6 can also be aberrantly Sumoylated by Sumo1 at lysines 122 and 142 during oxidative stress. Aberrant sumoylation of Prdx6 reduces its gene expression and protein abundance [24,25,26].…”
Section: Regulation Of the Enzymatic Activities Of Prdx6mentioning
confidence: 99%
“…The role of Prdx6 in ocular oxidative damage has been extensively investigated [91,92]. Prdx6 expression in the eye declines with age, increasing the risk of cataract formation [26,93,94]. Moreover, Prdx6 prevents oxidative stress in human retinal pigment epithelial cells by activating the Phosphoinositide 3-kinase/Protein kinase B (PI3K/AKT) pathway [95] and blunting transforming growth factor beta (TGF-β) signaling [96].…”
Section: Prdx6 Signaling In Diseasementioning
confidence: 99%
“…The role of PRDX6 in oxidative stress was described above in the introduction section. Decline of PRDX6 during aging has been associated with ocular pathobiology induced by age-related oxidative stress (Kubo et al, 2017; Chhunchha et al, 2018). Chhunchha et al (2017) proposed PRDX6 as a potential therapeutic target to protect the trabecular meshwork from age-dependent oxidative stress and accumulation of ROS by balancing redox-homeostasis to prevent ocular disorders.…”
Section: Discussionmentioning
confidence: 99%