Sulfur restriction extends fission yeast chronological lifespan through Ecl1 family genes by downregulation of ribosome

Ohtsuka, Hokuto; Takinami, Masahiro; Shimasaki, Takafumi; Hibi, Takayuki; Murakami, Hiroshi; Aiba, Hirofumi

doi:10.1111/mmi.13686

Cited by 23 publications

(77 citation statements)

References 43 publications

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“…Figure a shows that tschimganine also did not extend CLS in the crm1‐467 mutant. In contrast, diazaborine, which causes CLS extension in a CR‐like manner by inhibiting ribosome synthesis, remarkably extends CLS in the crm1‐467 mutant (Figure b; Loibl et al., ; Ohtsuka et al., ). This suggests that this mutant can absorb diazaborine, which is similar in molecular size to tschimganine (diazaborine: 300 g/mol and tschimganine: 290 g/mol).…”

Section: Resultsmentioning

confidence: 98%

“…Real‐time PCR analysis was performed as described previously (Ohtsuka et al., ) using the expression of cdc2 + as a quantitative control. The primers that were used are listed in Supporting Information Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…Meanwhile, many studies have reported trying to artificially regulate aging and life span using various drugs, including rapamycin, metformin, resveratrol and myriocin (Kaeberlein, Creevy, & Promislow, ; Kennedy et al., ; Liu et al., ; Santos, Leitão‐Correia, Sousa, & Leão, ). In Schizosaccharomyces pombe , studies regarding the effects of drugs on chronological life span (CLS) also have reported that various compounds, such as acivicin, tschimganine, diazaborine and ribozinoindole‐1, lead to life span extension (Ito et al., ; Ohtsuka et al., ; Stephan, Franke, & Ehrenhofer‐Murray, ).…”

Section: Introductionmentioning

confidence: 99%

“…); most of these are nutrient‐related signals and pathways. For example, pka1 + , sck2 + and ecl1 + are genes associated with CLS (Ohtsuka et al., ; Roux, Leroux et al., ; Roux, Quissac, Chartrand, Ferbeyre, & Rokeach, ). Among the mechanisms for CLS extension that are related to nutrient signals, the most commonly analyzed phenomenon is calorie restriction (CR).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have proposed a relationship between CR and translational activity; as such, the mechanism of longevity by CR is becoming clearer (MacInnes, ; Ohtsuka & Aiba, ; Steffen et al., ). For example, we have shown that sulfate limitation is a novel signal that extends CLS via the down‐regulation of ribosomal synthesis, as ribosomal activity is the determinant of CLS in fission yeast (Ohtsuka et al., ). In fission yeast, many reports regarding CLS are associated with the PKA pathway, the TOR pathway and the Ecl1 family genes, all of which are involved in the CR mechanism and the regulation of ribosomal activity; therefore, there are very few reports regarding CLS mediated independently of CR (Chen & Runge, ; Ohtsuka et al., ; Zuin et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Tschimganine and its derivatives extend the chronological life span of yeast via activation of the Sty1 pathway

et al. 2018

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Most antiaging factors or life span extenders are associated with calorie restriction (CR). Very few of these factors function independently of, or additively with, CR. In this study, we focused on tschimganine, a compound that was reported to extend chronological life span (CLS). Although tschimganine led to the extension of CLS, it also inhibited yeast cell growth. We acquired a Schizosaccharomyces pombe mutant with a tolerance for tschimganine due to the gene crm1. The resulting Crm1 protein appears to export the stress-activated protein kinase Sty1 from the nucleus to the cytosol even under stressful conditions. Furthermore, we synthesized two derivative compounds of tschimganine, α-hibitakanine and β-hibitakanine; these derivatives did not inhibit cell growth, as seen with tschimganine. α-hibitakanine extended the CLS, not only in S. pombe but also in Saccharomyces cerevisiae, indicating the possibility that life span regulation by tschimganine derivative may be conserved across various yeast species. We found that the longevity induced by tschimganine was dependent on the Sty1 pathway. Based on our results, we propose that tschimganine and its derivatives extend CLS by activating the Sty1 pathway in fission yeast, and CR extends CLS via two distinct pathways, one Sty1-dependent and the other Sty1-independent. These findings provide the potential for creating an additive life span extension effect when combined with CR, as well as a better understanding of the mechanism of CLS.

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tschimganine and its derivatives extend the chronological life span of yeast via activation of the Sty1 pathway

et al. 2018

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Low‐Molecular Weight Compounds that Extend the Chronological Lifespan of Yeasts, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

Ohtsuka,

Shimasaki,

Aiba

2024

Advanced Biology

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Yeast is an excellent model organism for research for regulating aging and lifespan, and the studies have made many contributions to date, including identifying various factors and signaling pathways related to aging and lifespan. More than 20 years have passed since molecular biological perspectives are adopted in this research field, and intracellular factors and signal pathways that control aging and lifespan have evolutionarily conserved from yeast to mammals. Furthermore, these findings have been applied to control the aging and lifespan of various model organisms by adjustment of the nutritional environment, genetic manipulation, and drug treatment using low‐molecular weight compounds. Among these, drug treatment is easier than the other methods, and research into drugs that regulate aging and lifespan is consequently expected to become more active. Chronological lifespan, a definition of yeast lifespan, refers to the survival period of a cell population under nondividing conditions. Herein, low‐molecular weight compounds are summarized that extend the chronological lifespan of Saccharomyces cerevisiae and Schizosaccharomyces pombe, along with their intracellular functions. The low‐molecular weight compounds are also discussed that extend the lifespan of other model organisms. Compounds that have so far only been studied in yeast may soon extend lifespan in other organisms.

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Magnesium depletion extends fission yeast lifespan via general amino acid control activation

et al. 2021

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Sulfur restriction extends fission yeast chronological lifespan through Ecl1 family genes by downregulation of ribosome

Cited by 23 publications

References 43 publications

Tschimganine and its derivatives extend the chronological life span of yeast via activation of the Sty1 pathway

Tschimganine and its derivatives extend the chronological life span of yeast via activation of the Sty1 pathway

Low‐Molecular Weight Compounds that Extend the Chronological Lifespan of Yeasts, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

Magnesium depletion extends fission yeast lifespan via general amino acid control activation

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