Magnesium depletion extends fission yeast lifespan via general amino acid control activation

Ohtsuka, Hokuto; Kobayashi, Mikuto; Shimasaki, Takafumi; Sato, Tomohiro; Akanuma, Genki; Kitaura, Yasuyuki; Otsubo, Yoko; Yamashita, Akira; Aiba, Hirofumi

doi:10.1002/mbo3.1176

Cited by 15 publications

(31 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 2017 ), suggesting that Ecl1 family genes act in response to various types of nutrient starvation. Ecl1 family genes are induced by multiple transcription factors, including Atf1, Fil1, Hsf1 and Zip1, which correspond to various individual stimuli (Ohtsuka et al 2019 , 2021 ).…”

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

“… 2020 ). In addition, under this condition, the levels of ribosomal factors also decrease in Ecl1 family gene-dependent manner (Ohtsuka et al 2017 , 2021 ). Similar to sulfur starvation, leucine starvation leads to ecl1 + induction, decreasing ribosome and CLS extension (Ohtsuka et al .…”

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

“…Similar to sulfur starvation, leucine starvation leads to ecl1 + induction, decreasing ribosome and CLS extension (Ohtsuka et al . 2019 , 2021 ). Using ribozinoindole-1, which inhibits ribosome biogenesis (Kawashima et al .…”

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

“…This suggests that the suppression of ribosome levels is necessary to maintain cell survival under leucine starvation (Ohtsuka et al . 2021 ). Similarly, it is considered that appropriately reducing the level of ribosomes, which can suppress metabolism and energy consumption under nutrient starvation, also contributes to the maintenance of cell survival under sulfur starvation.…”

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

See 3 more Smart Citations

Response to sulfur in Schizosaccharomyces pombe

Ohtsuka

Shimasaki

Aiba

2021

FEMS Yeast Research

Self Cite

View full text Add to dashboard Cite

Sulfur is an essential component of various biologically important molecules, including methionine, cysteine, and glutathione, and it is also involved in coping with oxidative and heavy metal stress. Studies using model organisms, including budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe), have contributed not only to understanding various cellular processes but also to understanding the utilization and response mechanisms of each nutrient, including sulfur. Although fission yeast can use sulfate as a sulfur source, its sulfur metabolism pathway is slightly different from that of budding yeast because it does not have a trans-sulfuration pathway. In recent years, it has been found that sulfur starvation causes various cellular responses in S. pombe, including sporulation, cell cycle arrest at G2, chronological lifespan extension, autophagy induction, and reduced translation. This MiniReview identifies two sulfate transporters in S. pombe, Sul1 (encoded by SPBC3H7.02) and Sul2 (encoded by SPAC869.05c), and summarizes the metabolic pathways of sulfur assimilation and cellular response to sulfur starvation. Understanding these responses, including metabolism and adaptation, will contribute to a better understanding of the various stress and nutrient starvation responses and chronological lifespan regulation caused by sulfur starvation.

show abstract

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

Section: Response To Sulfate Starvation In S Pombementioning

confidence: 99%

See 2 more Smart Citations

Response to sulfur in Schizosaccharomyces pombe

Ohtsuka

Shimasaki

Aiba

2021

FEMS Yeast Research

Self Cite

View full text Add to dashboard Cite

show abstract

“… 2014 , 2017 , 2020 , Ohtsuka et al . 2021 ). This protein acts on several cellular responses, including autophagy, cell cycle, translation, sexual differentiation, and the regulation of the chronological lifespan (Ohtsuka and Aiba 2017 , Shimasaki et al .…”

Section: Introductionmentioning

confidence: 99%

Response to leucine in Schizosaccharomyces pombe (fission yeast)

Ohtsuka

Shimasaki

Aiba

2022

FEMS Yeast Research

Self Cite

View full text Add to dashboard Cite

Leucine (Leu) is a branched-chain, essential amino acid in animals, including humans. Fungi, including the fission yeast Schizosaccharomyces pombe, can biosynthesize Leu, but deletion of any of the genes in this biosynthesis leads to Leu auxotrophy. In this yeast, although a mutation in the Leu biosynthetic pathway, leu1-32, is clearly inconvenient for this species, it has increased its usefulness as a model organism in laboratories worldwide. Leu auxotrophy produces intracellular responses and phenotypes different from those of the prototrophic strains, depending on the growing environment, which necessitates a certain degree of caution in the analysis and interpretation of the experimental results. Under amino acid starvation, the amino acid-auxotrophic yeast induces cellular responses, which are conserved in higher organisms without the ability of synthesizing amino acids. This mini-review focuses on the roles of Leu in S. pombe and discusses biosynthetic pathways, contribution to experimental convenience using a plasmid specific for Leu auxotrophic yeast, signaling pathways, and phenotypes caused by Leu starvation. An accurate understanding of the intracellular responses brought about by Leu auxotrophy can contribute to research in various fields using this model organism and to the understanding of intracellular responses in higher organisms that cannot synthesize Leu.

show abstract

Low‐Molecular Weight Compounds that Extend the Chronological Lifespan of Yeasts, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

Ohtsuka,

Shimasaki,

Aiba

2024

Advanced Biology

Self Cite

View full text Add to dashboard Cite

Yeast is an excellent model organism for research for regulating aging and lifespan, and the studies have made many contributions to date, including identifying various factors and signaling pathways related to aging and lifespan. More than 20 years have passed since molecular biological perspectives are adopted in this research field, and intracellular factors and signal pathways that control aging and lifespan have evolutionarily conserved from yeast to mammals. Furthermore, these findings have been applied to control the aging and lifespan of various model organisms by adjustment of the nutritional environment, genetic manipulation, and drug treatment using low‐molecular weight compounds. Among these, drug treatment is easier than the other methods, and research into drugs that regulate aging and lifespan is consequently expected to become more active. Chronological lifespan, a definition of yeast lifespan, refers to the survival period of a cell population under nondividing conditions. Herein, low‐molecular weight compounds are summarized that extend the chronological lifespan of Saccharomyces cerevisiae and Schizosaccharomyces pombe, along with their intracellular functions. The low‐molecular weight compounds are also discussed that extend the lifespan of other model organisms. Compounds that have so far only been studied in yeast may soon extend lifespan in other organisms.

show abstract

Magnesium depletion extends fission yeast lifespan via general amino acid control activation

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 15 publications

References 60 publications

Response to sulfur in Schizosaccharomyces pombe

Response to sulfur in Schizosaccharomyces pombe

Response to leucine in Schizosaccharomyces pombe (fission yeast)

Low‐Molecular Weight Compounds that Extend the Chronological Lifespan of Yeasts, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

Contact Info

Product

Resources

About