Sulfur-containing antioxidants increase in vitro several functions of lymphocytes from mice

Fuente, Mónica De la; Hernánz, Ángel; Viniegra, Salvador; Miquel, Jaime

doi:10.1016/j.intimp.2011.01.008

Cited by 8 publications

(8 citation statements)

References 51 publications

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“…In another in vitro study it was found that taurine, as a sulfur containing antioxidant, improves the functional capacity of lymphocytes in mice, which in turn enhances the immune response (De la Fuente, 2011). Moreover supplementation of taurine in deficient taurine animals leads to improve depletion of mature and immature B lymphocytes numbers and avoided the decline in T cells (Grimble, 1996).…”

Section: Discussionmentioning

confidence: 99%

Protective and therapeutic impact of taurine on some biochemical, immunological and histological parameters in diabetic rats

Foda¹,

Farrag²,

Metwally³

et al. 2016

J App Pharm Sci

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Background: Taurine is a non protein amino acid found in most animal tissues. It is a powerful antioxidant which shares in combating the harmful effect of the reactive oxygen species (ROS), associated to many chronic diseases as diabetes mellitus (DM). The disease is characterized by hyperglycemia and metabolic disorders in the body that leads to the release of ROS in the cells. Methods: The present work evaluates the biochemical and immunological role of taurine (500 mg/kg bwt) in ameliorating diabetes harm in rats when compared to the effect of the antidiabetic drug (amaryl). Six groups were established for the experiment. Group1: control rats without any supplementations. Group 2 : diabetic non treated rats. Group 3: rats received taurine for three weeks. Group 4: rats were supplemented with taurine for three weeks then injected streptozotocin (STZ) (prophylactic gp). Group 5: rats were injected with STZ then supplemented with taurine for four weeks (therapeutic gp). Group 6: rats were injected STZ then treated with amaryl drug for four weeks. Serum glucose and insulin levels in addition to liver function enzymes and lactate dehydrogenase enzyme were determined. ROS effect was monitored in liver tissue by detecting malondialdhyde resulting from lipid peroxidation and detecting glutathione reductase enzyme activity. With respect to the immunological responses, the thymocytes and splenocytes numbers were counted besides measuring serum IgG level. Histological and immunohistochemical studies were performed in pancreatic sections. Results: showed the ability of taurine in decreasing glucose level and increasing insulin with the same efficacy as amaryl drug besides affecting liver enzymes and improving the antioxidant system in cells. Taurine also restored the decrease in mean number of thymocytes and splenocytes caused by DM. Sera IgG levels from pre-and post-treatment with taurine showed non significant increase compared to the diabetic non treated group. Conclusion: post-treatment supplemention of taurine is recommended for T2DM.

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Section: Discussionmentioning

confidence: 99%

Protective and therapeutic impact of taurine on some biochemical, immunological and histological parameters in diabetic rats

Foda¹,

Farrag²,

Metwally³

et al. 2016

J App Pharm Sci

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“…Although not specifically tested in developing B cells, NAC has been shown to inhibit apoptosis and potentiate proliferation in cell culture. Specifically, Rosati et al reported that NAC inhibits the spontaneous apoptosis of human tonsillar B lymphocytes in vitro by inhibiting the processing of caspase-3 and -7 [15], and De la Fuente et al showed that NAC enhanced the proliferation of leukocytes stimulated with concavalin A in vitro [16]. Since the number of B220 + AA4.1 + sIgM − sIgD − B cells with low and high FSC profiles are both increased by NAC treatment, the evidence suggests NAC treatment does not uniquely affect an actively proliferating B cell subset (Fig.…”

Section: Discussionmentioning

confidence: 99%

N-Acetylcysteine Increases the Frequency of Bone Marrow Pro-B/Pre-B Cells, but Does Not Reverse Cigarette Smoking-Induced Loss of This Subset

et al. 2011

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BackgroundWe previously showed that mice exposed to cigarette smoke for three weeks exhibit loss of bone marrow B cells at the Pro-B-to-pre-B cell transition, but the reason for this is unclear. The antioxidant N-acetylcysteine (NAC), a glutathione precursor, has been used as a chemopreventive agent to reduce adverse effects of cigarette smoke exposure on lung function. Here we determined whether smoke exposure impairs B cell development by inducing cell cycle arrest or apoptosis, and whether NAC treatment prevents smoking-induced loss of developing B cells.Methodology/Principal FindingsGroups of normal mice were either exposed to filtered room air or cigarette smoke with or without concomitant NAC treatment for 5 days/week for three weeks. Bone marrow B cell developmental subsets were enumerated, and sorted pro-B (B220+CD43+) and pre-B (B220+CD43−) cell fractions were analyzed for cell cycle status and the percentage of apoptotic cells. We find that, compared to sham controls, smoke-exposed mice have ∼60% fewer pro-B/pre-B cells, regardless of NAC treatment. Interestingly, NAC-treated mice show a 21–38% increase in total bone marrow cellularity and lymphocyte frequency and about a 2-fold increase in the pro-B/pre-B cell subset, compared to sham-treated controls. No significant smoking- or NAC-dependent differences were detected in frequency of apoptotic cells or the percentage cells in the G1, S, or G2 phases of the cycle.Conclusions/SignificanceThe failure of NAC treatment to prevent smoking-induced loss of bone marrow pre-B cells suggests that oxidative stress is not directly responsible for this loss. The unexpected expansion of the pro-B/pre-B cell subset in response to NAC treatment suggests oxidative stress normally contributes to cell loss at this developmental stage, and also reveals a potential side effect of therapeutic administration of NAC to prevent smoking-induced loss of lung function.

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“…It has been reported to induce respiratory burst in flatfish and mammalian phagocytes (Castro et al 2004(Castro et al , 2006Leiro et al 2007;van Rooijen and Sanders 1997). Phagocyte activation is a key process in the host defence against microorganisms, and it is mostly related with the anti-oxidant properties already recognised in this polysaccharide (Castro et al 2004(Castro et al , 2006De la Fuente et al 2011;Leiro et al 2007). This activity is strongly influenced by the presence of sulphate groups as well as by exposure time and concentration and may occur through interaction of the polysaccharide with cell surface receptors (Castro et al 2004(Castro et al , 2006De la Fuente et al 2011;Leiro et al 2007).…”

Section: Biological Propertiesmentioning

confidence: 99%

“…Phagocyte activation is a key process in the host defence against microorganisms, and it is mostly related with the anti-oxidant properties already recognised in this polysaccharide (Castro et al 2004(Castro et al , 2006De la Fuente et al 2011;Leiro et al 2007). This activity is strongly influenced by the presence of sulphate groups as well as by exposure time and concentration and may occur through interaction of the polysaccharide with cell surface receptors (Castro et al 2004(Castro et al , 2006De la Fuente et al 2011;Leiro et al 2007). Ulvan can also mediate intestinal epithelial growth and take part on the repair of wounds by stabilising and promoting binding of relevant growth factors to intestinal cells (Warrand 2006).…”

Section: Biological Propertiesmentioning

confidence: 99%

A practical perspective on ulvan extracted from green algae

2012

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Researchers have many times turned their attention to nature and biological processes to develop novel technologies and materials. In a medical perspective, nature-based products are believed to be a strategic alternative approach to the use of fully synthetic materials, particularly in the design of medical devices. In the past decades, marine organisms have become the focus of considerable attention as potential sources of valuable materials. The sustainable exploitation and valorisation of natural marine resources constitutes a highly attractive and strategic platform for the development of novel biomaterials, with both economic and environmental benefits. In this context, algae are known to synthesise large quantities of polysaccharides and are well established sources of these particularly interesting molecules, many of which are known for their applicability in the design of biomaterials. Agar, carrageenan and alginates are some of the most known examples, and their uses can range from food to biomedical applications. However, few of the world's available seaweed species are used commercially. Among the three main divisions of macroalgae (Chlorophyta, Phaeophyta and Rhodophyta), the green algae remain largely unexploited in this biomedical arena. While the demand for novel materials and technologies increases, so does the research of unexploited marine green algae including its unique polysaccharide ulvan.

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Sulfur-containing antioxidants increase in vitro several functions of lymphocytes from mice

Cited by 8 publications

References 51 publications

Protective and therapeutic impact of taurine on some biochemical, immunological and histological parameters in diabetic rats

Protective and therapeutic impact of taurine on some biochemical, immunological and histological parameters in diabetic rats

N-Acetylcysteine Increases the Frequency of Bone Marrow Pro-B/Pre-B Cells, but Does Not Reverse Cigarette Smoking-Induced Loss of This Subset

A practical perspective on ulvan extracted from green algae

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