2021
DOI: 10.18632/aging.202512
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Abstract: The broccoli-derived isothiocyanate sulforaphane inhibits inflammation, oxidative stress and cancer, but its effect on healthspan and longevity are unclear. We used the C. elegans nematode model and fed the wildtype and 9 mutant strains ±sulforaphane. The lifespan, phenotype, pharyngeal pumping, mobility, lipofuscin accumulation, and RNA and protein expression of the nematodes were assessed by using Kaplan-Meier survival analysis, in vivo live imaging, fluorescence… Show more

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Cited by 27 publications
(10 citation statements)
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“…To investigate the impact of sulforaphane, which is chemically a sulfur-containing isothiocyanate ( Figure 1A ), we fed synchronized L4 wild-type C. elegans larvae with E. coli OP50 food bacteria in the presence or absence of 100 µM sulforaphane. We chose this sulforaphane concentration because it is considered optimum to treat C. elegans ( Qi et al, 2021 ). The number of worms was recorded daily until no more live worms could be found from Day 33 onwards.…”
Section: Resultsmentioning
confidence: 99%
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“…To investigate the impact of sulforaphane, which is chemically a sulfur-containing isothiocyanate ( Figure 1A ), we fed synchronized L4 wild-type C. elegans larvae with E. coli OP50 food bacteria in the presence or absence of 100 µM sulforaphane. We chose this sulforaphane concentration because it is considered optimum to treat C. elegans ( Qi et al, 2021 ). The number of worms was recorded daily until no more live worms could be found from Day 33 onwards.…”
Section: Resultsmentioning
confidence: 99%
“…Lifespan assays were performed as recently described ( Qi et al, 2021 ). For synchronization, 30 gravid C. elegans worms were transferred to new NGM plates supplemented with E. coli OP50 food bacteria to lay eggs for 3 h. Next, the worms were picked and washed.…”
Section: Methodsmentioning
confidence: 99%
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“…Furthermore, sulforaphane treatment in a human prostate cancer cell lines impeded cancer cell survival via H 2 S-mediated JNK and MAPK signalling [ 159 ]. Finally, the activity of sulforaphane was attributed largely to its potent activation of NRF2 by modification of KEAP1 [ 160 ] and insulin signalling [ 161 ], mechanisms that are also directly influenced by H 2 S signalling (see sections Post-translational modification (persulfidation) and Acarbose and IIS signalling). Given that sulforaphane is a compound that is essentially a naturally occurring H 2 S donor and has been shown to operate through biological mechanisms that are known H 2 S signalling pathways, there have been a surprisingly limited number of studies that directly monitor H 2 S levels following sulforaphane treatment, and none in the context of HGPS.…”
Section: H 2 S and Ageingmentioning
confidence: 99%