Sulforaphane but not ascorbigen, indole‐3‐carbinole and ascorbic acid activates the transcription factor Nrf2 and induces phase‐2 and antioxidant enzymes in human keratinocytes in culture

Wagner, Anika E.; Ernst, Insa M. A.; Iori, Renato; Desel, Christine; Rimbach, Gerald

doi:10.1111/j.1600-0625.2009.00928.x

Cited by 91 publications

(65 citation statements)

References 40 publications

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“…Liver nuclear and cytoplasmatic extracts were prepared as described in Wagner et al [41]. Owing to the limited excretion of protein in the urine and the resulting lack of a loading control, protein concentrations in urinary samples were determined with the Coomassie plus protein assay kit (Thermo Scientific, Schwerte, Germany) according to the manufacturer's instructions to ensure equal loading amounts of protein onto the gel.…”

Section: (D) Western Blot Analysismentioning

confidence: 99%

Major urinary protein 5, a scent communication protein, is regulated by dietary restriction and subsequent re-feeding in mice

et al. 2013

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Major urinary proteins (Mups) are important for rodent scent communication and sexual behaviour. Recent evidence suggests that Mup1 may be regulated by fasting and re-feeding (RF). However, other Mup isoforms are poorly investigated, and data on the impact of long-term dietary restriction (DR) and ad libitum RF on Mup expression are missing. We investigated the effects of long-term 25 per cent DR and subsequent RF on Mup expression in male C57BL6 mice. DR significantly decreased Mup gene expression, hepatic and urinary protein levels compared with ad libitum (AL) fed control mice, with the greatest downregulation found for Mup5 expression. The decline in Mup expression was inverted by six months of RF. Because of inhibitory glucocorticoid response elements in the genomic sequence of the Mup5 gene, the observed inverse correlation of nuclear glucocorticoid receptor levels with Mup expression in response to DR and subsequent RF is a possible regulatory mechanism. Additionally, gene-expression-inhibiting histone deacetylation (H3K9) occurred in the region of the Mup5 gene in response to DR. We assume that Mup may act as a molecular switch linking nutritional status to sexual behaviour of mice, and thereby regulating male fertility and reproduction in response to food supply.

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Section: (D) Western Blot Analysismentioning

confidence: 99%

Major urinary protein 5, a scent communication protein, is regulated by dietary restriction and subsequent re-feeding in mice

et al. 2013

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“…Protein concentrations were determined in the supernatants by the bicinchoninic acid assay (Pierce, IL, USA). Protein (60 mg) was separated on a 12 % SDS-polyacrylamide gel and transferred onto an immunoblot polyvinylidenefluoride membrane (12) . The membrane was blocked with 3 % non-fat dried milk in Tris-buffered saline, pH 7·4, with 0·05 % Tween-20 (TBS/T) for 2 h and probed with a polyclonal rabbit anti-PON1 antibody (1:1000; Abcam, Cambridge, UK) at 48C overnight.…”

Section: Western Blottingmentioning

confidence: 99%

Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

et al. 2010

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Paraoxonase 1 (PON1) is an enzyme that is mainly synthesised in the liver and protects LDL from oxidation, thereby exhibiting antiatherogenic properties. Using a luciferase reporter gene assay, we tested curcumin for its ability to induce PON1 in Huh7 hepatocytes in culture. Curcumin ($10 mmol/l) dose-dependently induced PON1 transactivation in Huh7 cells. However, dietary supplementation of female B6C3F1 mice with curcumin (500 mg/kg diet) for 2 weeks did not increase the hepatic PON1 mRNA and protein levels. No curcumin was detectable in the plasma of the 12 h fasted mice. In conclusion, curcumin may be a potent PON1 inducer in cultured cells in vitro, but not in the liver of curcumin-fed mice because of its low concentrations in vivo.

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“…The purity of glucosinolate was subsequently improved via gelfiltration conducted using a XK 26/100 column packed with Sephadex G10 chromatography media (Amersham Biosciences), coupled with FPLC System (Pharmacia). All fractions were assessed by HPLC for pure glucosinolates followed by freeze-drying (Wagner et al, 2010). Finally, glucosinolates were characterised employing NMR spectrometry and the purity was checked using HPLC analysis according to the ISO 9167-1 method (EEC Regulation, 1990).…”

Section: Glucosinolates Isolationmentioning

confidence: 99%

Naturally-Occurring Glucosinolates, Glucoraphanin and Glucoerucin, are Antagonists to Aryl Hydrocarbon Receptor as Their Chemopreventive Potency

Razis

Noor²

2015

Asian Pacific Journal of Cancer Prevention

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As a cytosolic transcription factor, the aryl hydrocarbon (Ah) receptor is involved in several pathophysiological events leading to immunosuppression and cancer; hence antagonists of the Ah receptor may possess chemoprevention properties. It is known to modulate carcinogen-metabolising enzymes, for instance the CYP1 family of cytochromes P450 and quinone reductase, both important in the biotransformation of many chemical carcinogens via regulating phase I and phase II enzyme systems. Utilising chemically-activated luciferase expression (CALUX) assay it was revealed that intact glucosinolates, glucoraphanin and glucoerucin, isolated from Brassica oleracea L. var. acephala sabellica and Eruca sativa ripe seeds, respectively, are such antagonists. Both glucosinolates were poor ligands for the Ah receptor; however, they effectively antagonised activation of the receptor by the avid ligand benzo[a]pyrene. Indeed, intact glucosinolate glucoraphanin was a more potent antagonist to the receptor than glucoerucin. It can be concluded that both glucosinolates effectively act as antagonists for the Ah receptor, and this may contribute to their established chemoprevention potency.

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Sulforaphane but not ascorbigen, indole‐3‐carbinole and ascorbic acid activates the transcription factor Nrf2 and induces phase‐2 and antioxidant enzymes in human keratinocytes in culture

Cited by 91 publications

References 40 publications

Major urinary protein 5, a scent communication protein, is regulated by dietary restriction and subsequent re-feeding in mice

Major urinary protein 5, a scent communication protein, is regulated by dietary restriction and subsequent re-feeding in mice

Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo

Naturally-Occurring Glucosinolates, Glucoraphanin and Glucoerucin, are Antagonists to Aryl Hydrocarbon Receptor as Their Chemopreventive Potency

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