Sulforaphane and 2-oxohexyl isothiocyanate induce cell growth arrest and apoptosis in L-1210 leukemia and ME-18 melanoma cells

Misiewicz, Irena; Skupińska, Katarzyna; Kasprzycka‐Guttman, T.

doi:10.3892/or.10.6.2045

Cited by 29 publications

(34 citation statements)

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“…Cell growth inhibition and apoptosis induction by sulforaphane in SV40-transformed MEFs (present study) as well as in other cells, including colon, Jurkat T leukemia, and prostate cancer cells, have been observed at 10 to 40 Amol/L concentrations (20)(21)(22)(23)(24)(25)(26)(27)(28)(29). A fundamental question, which remains unanswered, is whether the micromolar concentrations of sulforaphane needed to trigger cell death are achievable in humans.…”

Section: Discussionmentioning

confidence: 96%

“…Studies have indicated that inhibitory effect of sulforaphane against proliferation of cultured cancer cells is attributable to cell cycle block as well as apoptosis induction (20)(21)(22)(23)(24)(25)(26)(27)(28)(29). A novel mechanism of chemoprevention by sulforaphane involving inhibition of histone deacetylase was also suggested recently (26).…”

Section: Discussionmentioning

confidence: 99%

“…Evidence is accumulating to indicate that sulforaphane not only offers protection against chemically induced cancer in animal models (15,17,18) but also suppresses proliferation of cancer cells in culture as well as in vivo (20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Studies have indicated that inhibitory effect of sulforaphane against proliferation of cultured cancer cells is attributable to cell cycle block as well as apoptosis induction (20)(21)(22)(23)(24)(25)(26)(27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

“…Growth inhibition, cell cycle arrest, and/or apoptosis induction by sulforaphane has been documented in human colon, leukemia, medulloblastoma, and prostate cancer cells (20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Gamet-Payrastre et al (20) were the first to show that treatment of HT29 human colon cancer cells with sulforaphane resulted in G 2 -M-phase cell cycle arrest and apoptosis induction.…”

Section: Introductionmentioning

confidence: 99%

“…More recent studies, including those from our laboratory, have indicated that sulforaphane can suppress proliferation of cancer cells in culture and in vivo by inhibiting cell cycle progression and/ or causing apoptosis induction (20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Growth inhibition, cell cycle arrest, and/or apoptosis induction by sulforaphane has been documented in human colon, leukemia, medulloblastoma, and prostate cancer cells (20)(21)(22)(23)(24)(25)(26)(27)(28)(29).…”

Section: Introductionmentioning

confidence: 99%

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Bax and Bak Are Required for Apoptosis Induction by Sulforaphane, a Cruciferous Vegetable–Derived Cancer Chemopreventive Agent

2005

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Sulforaphane, a constituent of many edible cruciferous vegetables, including broccoli, effectively suppresses proliferation of cancer cells in culture and in vivo by causing apoptosis induction, but the sequence of events leading to cell death is poorly defined. Here, we show that multidomain proapoptotic Bcl-2 family members Bax and Bak play a critical role in apoptosis induction by sulforaphane. This conclusion is based on the following observations: (a) sulforaphane treatment caused a dose-and time-dependent increase in the protein levels of both Bax and Bak and conformational change and mitochondrial translocation of Bax in SV40-transformed mouse embryonic fibroblasts (MEF) derived from wild-type mice to trigger cytosolic release of apoptogenic molecules (cytochrome c and Smac/DIABLO), activation of caspase-9 and caspase-3, and ultimately cell death; (b) MEFs derived from Bax or Bak knockout mice resisted cell death by sulforaphane, and (c) MEFs derived from Bax and Bak double knockout mice exhibited even greater protection against sulforaphane-induced cytochrome c release, caspase activation, and apoptosis compared with wild-type or single knockout cells. Interestingly, sulforaphane treatment also caused a dose-and time-dependent increase in the protein level of Apaf-1 in wild-type, Bax

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bax and Bak Are Required for Apoptosis Induction by Sulforaphane, a Cruciferous Vegetable–Derived Cancer Chemopreventive Agent

2005

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Sulphur‐Containing Compounds

Mithen¹

2006

Plant Secondary Metabolites

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Inhibition of cell cycle and induction of apoptosis by 2-oxoheksyl isothiocyanate and alyssin in cell lines carrying various inheritedBRCA1 mutations

Misiewicz

Kozar²,

Skupińska

et al. 2005

Drug Dev. Res.

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An important aspect of the chemopreventive activity of isothiocyanates (ITC) is their ability to induce cell growth inhibition and apoptosis. In this study, the effect of two sulforaphane analogues, 2-oxoheksyl isothiocyanate and alyssin, on lymphoblastoid cells, derived from people carrying four different germ-line mutations in BRCA1 gene, was tested and compared to the effect on wild type cells. The mutations studied were: C61G; 3819del5; 4153delA, and 5382INSC. Changes in cell viability and density after 2-oxoheksyl isothiocyanate and alyssin treatment were evaluated, as well as cell cycle progression, mitochondrial membrane potential changes, and phosphatidylserine externalization. Both isothiocyanates were shown to reduce cell viability and density in all cell lines tested, as well as the change in cell cycle phase's distribution. The response of cells to two ITC tested was various, as well as mutation type-modulated. We found that change of cellular maintenance by chemopreventive agents can be modulated by single allele BRCA1 mutation. Drug Dev. Res. 65:84-92, 2005.

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Sulforaphane and 2-oxohexyl isothiocyanate induce cell growth arrest and apoptosis in L-1210 leukemia and ME-18 melanoma cells

Cited by 29 publications

References 0 publications

Bax and Bak Are Required for Apoptosis Induction by Sulforaphane, a Cruciferous Vegetable–Derived Cancer Chemopreventive Agent

Bax and Bak Are Required for Apoptosis Induction by Sulforaphane, a Cruciferous Vegetable–Derived Cancer Chemopreventive Agent

Sulphur‐Containing Compounds

Inhibition of cell cycle and induction of apoptosis by 2-oxoheksyl isothiocyanate and alyssin in cell lines carrying various inheritedBRCA1 mutations

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