The number of women on military missions has been increasing recently. While on military missions, they live in an isolated group dominated by men. Such groups consist of young, sexually active individuals, thus increasing the risk of sexually transmitted infections.Medical examinations were performed on various residual forces (military, police, security guards) totaling 579 individuals, up to 35 years of age (172 women and 407 men). Subjects qualifi ed for the study were divided into 2 groups. Group I consisted of persons who remained in the country. Group II included persons stationed on military missions abroad. A total of 306 soldiers from this group participated in Study 1. Study 2 (clinical and laboratory) was carried out 6-12 months later on 52 patients from Group II and 119 patients from Group I (84 women and 87 men). Additionally, transcription of HPV genes E6 and E7 (with high oncogenic risk) was carried out in the group made up of 200 women. Laboratory examinations included: peripheral blood morphology, lymphocyte subpopulations by fl ow cytometry method (CD45, CD3, CD19, CD4, CD8 markers and NK cells CD16+CD56 markers), IL-2, IL-4, TNF-α, IFN-γ, IL-18 concentration by fl ow cytometry method. IgG, IgA, IgM concentration by turbidimetric method and
An important aspect of the chemopreventive activity of isothiocyanates (ITC) is their ability to induce cell growth inhibition and apoptosis. In this study, the effect of two sulforaphane analogues, 2-oxoheksyl isothiocyanate and alyssin, on lymphoblastoid cells, derived from people carrying four different germ-line mutations in BRCA1 gene, was tested and compared to the effect on wild type cells. The mutations studied were: C61G; 3819del5; 4153delA, and 5382INSC. Changes in cell viability and density after 2-oxoheksyl isothiocyanate and alyssin treatment were evaluated, as well as cell cycle progression, mitochondrial membrane potential changes, and phosphatidylserine externalization. Both isothiocyanates were shown to reduce cell viability and density in all cell lines tested, as well as the change in cell cycle phase's distribution. The response of cells to two ITC tested was various, as well as mutation type-modulated. We found that change of cellular maintenance by chemopreventive agents can be modulated by single allele BRCA1 mutation. Drug Dev. Res. 65:84-92, 2005.
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