Sulfidation of silver nanowires inside human alveolar epithelial cells: a potential detoxification mechanism

Chen, Shu; Goode, Angela E.; Sweeney, Sinbad; Theodorou, Ioannis G.; Thorley, Andrew J.; Ruenraroengsak, Pakatip; Chang, Yan; Gow, Andrew J.; Schwander, Stephan; Skepper, Jeremy N.; Zhang, Junfeng Jim; Shaffer, Milo S. P.; Chung, Kian Fan; Tetley, Teresa D.; Ryan, Mary P.; Porter, Alexandra E.

doi:10.1039/c3nr03205a

Cited by 59 publications

(116 citation statements)

References 43 publications

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“…Silver ions released by dissolution of Ag nanowires inside epithelial cells have been observed to form an insoluble Ag 2 S precipitate. 39,40 The measurements reported here are associated with a National Institute of Environmental Health Sciences (NIEHS) Centers for Nanotechnology Health Implications Research (NCNHIR) consortium examining the toxicology of nanoparticles. Specifically, 20 and 110 nm particles grown using 7-8 nm Au seed particles having citrate coatings were prepared by NanoComposix (San Diego, CA) for consortium use.…”

Section: Introductionmentioning

confidence: 99%

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

et al. 2015

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Widespread use of silver nanoparticles raises questions of environmental and biological impact. Many synthesis approaches are used to produce pure silver and silver-shell gold-core particles optimized for specific applications. Since both nanoparticles and silver dissolved from the particles may impact the biological response, it is important to understand the physicochemical characteristics along with the biological impact of nanoparticles produced by different processes. The authors have examined the structure, dissolution, and impact of particle exposure to macrophage cells of two 20 nm silver particles synthesized in different ways, which have different internal structures. The structures were examined by electron microscopy and dissolution measured in Rosewell Park Memorial Institute media with 10% fetal bovine serum. Cytotoxicity and oxidative stress were used to measure biological impact on RAW 264.7 macrophage cells. The particles were polycrystalline, but 20 nm particles grown on gold seed particles had smaller crystallite size with many high-energy grain boundaries and defects, and an apparent higher solubility than 20 nm pure silver particles. Greater oxidative stress and cytotoxicity were observed for 20 nm particles containing the Au core than for 20 nm pure silver particles. A simple dissolution model described the time variation of particle size and dissolved silver for particle loadings larger than 9 μg/ml for the 24-h period characteristic of many in-vitro studies.

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Section: Introductionmentioning

confidence: 99%

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

et al. 2015

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“…Figure 2b shows the morphology change of AgNWs inside cells, which have been processed using methods which do not alter their physicochemical properties. (Chen et al, 2013a, Chen et al , 2013c) It is important to highlight that the resin embedding and curing does not create any artefacts during this preparation procedure. The AgNWs have lost their original intact structures with small particles surrounding and decorating the AgNWs surface, after 24 hours incubation with transformed human alveolar type 1-like (TT1) cells.…”

Section: Overview Of Processing Of Resin-embedded Samples For Temmentioning

confidence: 99%

“…(Zhang et al , 2012) We suggested that sulfidation in such systems can act as a potential detoxification mechanism by trapping free Ag + ions. (Chen et al, 2013a)…”

Section: The Stability Of Agnms In Biological Solutions: the Effects mentioning

confidence: 99%

“…Electron microscopy (EM) images provide spatially resolved information about interfaces between nanomaterials and proteins (Chen et al , 2012, Mahmoudi et al , 2014) (Figure 1a), lipids (Leo et al , 2013) (Figure 1b), cells (Chen et al , 2013a)(Figure 1c–d), and tissues (Loeschner et al , 2011). When applied in combination with analytical and diffraction techniques, changes in the physicochemistry of AgNMs can be mapped at the sub-nano- to micro-meter length scales.…”

Section: Introductionmentioning

confidence: 99%

“…When applied in combination with analytical and diffraction techniques, changes in the physicochemistry of AgNMs can be mapped at the sub-nano- to micro-meter length scales. (Chen et al, 2013a) However, conventional biological sample preparation protocols for the transmission electron microscope (TEM) can be extremely complex. Cells exposed to AgNMs can be processed in many different chemicals to fix the proteinaceous constituents of the cells.…”

Section: Introductionmentioning

confidence: 99%

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Avoiding artefacts during electron microscopy of silver nanomaterials exposed to biological environments

Chen

Goode

Skepper

et al. 2015

Journal of Microscopy

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Electron microscopy has been applied widely to study the interaction of nanomaterials with proteins, cells and tissues at nanometre scale. Biological material is most commonly embedded in thermoset resins to make it compatible with the high vacuum in the electron microscope. Room temperature sample preparation protocols developed over decades provide contrast by staining cell organelles, and aim to preserve the native cell structure. However, the effect of these complex protocols on the nanomaterials in the system is seldom considered. Any artefacts generated during sample preparation may ultimately interfere with the accurate prediction of the stability and reactivity of the nanomaterials. As a case study, we review steps in the room temperature preparation of cells exposed to silver nanomaterials (AgNMs) for transmission electron microscopy imaging and analysis. In particular, embedding and staining protocols, which can alter the physicochemical properties of AgNMs and introduce artefacts thereby leading to a misinterpretation of silver bioreactivity, are scrutinised. Recommendations are given for the application of cryogenic sample preparation protocols, which simultaneously fix both particles and diffusible ions. By being aware of the advantages and limitations of different sample preparation methods, compromises or selection of different correlative techniques can be made to draw more accurate conclusions about the data.

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A Biomimetic Multifunctional Scaffold for Infectious Vertical Bone Augmentation

Zhang,

Li,

Guo

et al. 2024

Advanced Science

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The regenerative treatment of infectious vertical bone defects remains difficult and challenging today. Current clinical treatments are limited in their ability to control bacteria and infection, which is unfavorable for new bone formation and calls for a new type of material with excellent osteogenic and antibacterial properties. Here a multifunctional scaffold is synthesized that mimics natural bone nanostructures by incorporating silver nanowires into a hierarchical, intrafibrillar mineralized collagen matrix (IMC/AgNWs), to achieve the therapeutic goals of inhibiting bacterial activity and promoting infectious alveolar bone augmentation in rats and beagle dogs. An appropriate concentration of 0.5 mg mL−1 AgNWs is selected to balance biocompatibility and antibacterial properties. The achieved IMC/AgNWs exhibit a broad spectrum of antimicrobial properties against Gram‐negative Porphyromonas gingivalis and Gram‐positive Streptococcus mutans. When the IMC/AgNWs are cocultured with periodontal ligament stem cells, it possesses excellent osteoinductive activities under both non‐inflammatory and inflammatory conditions. By constructing a rat mandibular infected periodontal defect model, the IMC/AgNWs achieve a near‐complete healing through the canonical BMP/Smad signaling. Moreover, the IMC/AgNWs enhance vertical bone height and osseointegration in peri‐implantitis in beagle dogs, indicating the clinical translational potential of IMC/AgNWs for infectious vertical bone augmentation.

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Sulfidation of silver nanowires inside human alveolar epithelial cells: a potential detoxification mechanism

Cited by 59 publications

References 43 publications

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

Avoiding artefacts during electron microscopy of silver nanomaterials exposed to biological environments

A Biomimetic Multifunctional Scaffold for Infectious Vertical Bone Augmentation

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