Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

Munusamy, Prabhakaran; Wang, Chongmin; Engelhard, Mark H.; Baer, Donald R.; Smith, Jordan N.; Liu, Chongxuan; Kodali, Vamsi; Thrall, Brian D.; Chen, Shu; Porter, Alexandra E.; Ryan, Mary P.

doi:10.1116/1.4926547

Cited by 27 publications

(50 citation statements)

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“…This may suggest an inflammatory response to IT instillation of AgNP that does not remain localized to the lungs. The apparent interactions of AgNP with macrophages observed in this study and reported elsewhere [12,23,36–38] may promote cytokine release in the lungs resulting in a systemic inflammatory response through these cytokines. AgNP have been demonstrated to interact with mast cells and induce degranulation [8].…”

Section: Discussionsupporting

confidence: 69%

“…This may result from slowed pulmonary clearance of particles administered by instillation compared to inhalation [45] or the dissolution and formulation of other NP or salt participates [24,36,46] The contribution of silver ions to the observed toxicity observed remains a possible mechanism. It has been reported that 20 nm citrate capped AgNP quickly dissolutes in water however, this dissolution can be slowed in the presence of dipalmitoylphosphatidylcholine a componenet of surfactant [23,47].…”

Section: Discussionmentioning

confidence: 99%

“…Reports from the NIH Centers for Nanotechnology Health Implications Research Consortium meetings suggest that the dissolution rate from theses nanomaterials in in vitro settings can range from 1–30% dependent on the particle size and the initial suspension concentration [23]. Studies regarding the dissolution of silver ions from AgNP are difficult to conduct, however studies have evaluated the Ag dissolution rates of species of AgNP in culture media [36]. Our assessment of the cardiovascular impact of the IT of silver acetate at least for 1 day post exposure, was not equivalent in its impact on cytokine and vascular reactivity as that observed for AgNP exposure and may suggest other mechanisms for ionic silver related CV toxicity than those associated with AgNP.…”

Section: Discussionmentioning

confidence: 99%

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Cardiac Ischemia Reperfusion Injury Following Instillation of 20 nm Citrate-capped Nanosilver

DP¹,

Shannahan²

2015

J Nanomedic Nanotechnol

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Background Silver nanoparticles (AgNP) have garnered much interest due to their antimicrobial properties, becoming one of the most utilized nano-scale materials. However, any potential evocable cardiovascular injury associated with exposure has not been reported to date. We have previously demonstrated expansion of myocardial infarction after intratracheal (IT) instillation of carbon-based nanomaterials. We hypothesized pulmonary exposure to Ag core AgNP induces a measureable increase in circulating cytokines, expansion of cardiac ischemia-reperfusion (I/R) injury and is associated with depressed coronary constrictor and relaxation responses. Secondarily, we addressed the potential contribution of silver ion release on AgNP toxicity. Methods Male Sprague-Dawley rats were exposed to 200 μl of 1 mg/ml of 20 nm citrate-capped Ag core AgNP, 0.01, 0.1, 1 mg/ml Silver Acetate (AgAc), or a citrate vehicle by intratracheal (IT) instillation. One and 7 days following IT instillation the lungs were evaluated for inflammation and the presence of silver; serum was analyzed for concentrations of selected cytokines; cardiac I/R injury and coronary artery reactivity were assessed. Results AgNP instillation resulted in modest pulmonary inflammation with detection of silver in lung tissue and alveolar macrophages, elevation of serum cytokines: G-CSF, MIP-1α, IL-1β, IL-2, IL-6, IL-13, IL-10, IL-18, IL-17α, TNFα, and RANTES, expansion of I/R injury and depression of the coronary vessel reactivity at 1 day post IT compared to vehicle treated rats. Silver within lung tissue was persistent at 7 days post IT instillation and was associated with an elevation in cytokines: IL-2, IL-13, and TNFα and expansion of I/R injury. AgAc resulted in a concentration dependent infarct expansion and depressed vascular reactivity without marked pulmonary inflammation or serum cytokine response. Conclusions Based on these data, IT instillation of AgNP increases circulating levels of several key cytokines, which may contribute to persistent expansion of I/R injury possibly through an impaired vascular responsiveness.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cardiac Ischemia Reperfusion Injury Following Instillation of 20 nm Citrate-capped Nanosilver

DP¹,

Shannahan²

2015

J Nanomedic Nanotechnol

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“…The particles had been stabilized by sodium citrate and were also covered, as should be expected, by a thin layer of adventitious hydrocarbon. Scanning transmission electron microscopy (STEM) analysis of a series of particles showed that the core/shell Au/Ag NPs, described previously, 7 had a polydispersed size distribution, often had slightly non-spherical shapes, and had Au cores that were frequently off-center. The STEM measurements were used to determine average dimensions of the Au core and Ag shell as well as the distribution of deviations from that average.…”

mentioning

confidence: 73%

Quantifying the Impact of Nanoparticle Coatings and Nonuniformities on XPS Analysis: Gold/Silver Core–Shell Nanoparticles

Wang¹,

Engelhard

Baer

et al. 2016

Anal. Chem.

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Spectral modeling of photoelectrons can serve as a valuable tool when combined with X-ray photoelectron spectroscopy (XPS) analysis. Herein, a new version of the NIST Simulation of Electron Spectra for Surface Analysis (SESSA 2.0) software, capable of directly simulating spherical multilayer NPs, was applied to model citrate stabilized Au/Ag-core/shell nanoparticles (NPs). The NPs were characterized using XPS and scanning transmission electron microscopy (STEM) to determine the composition and morphology of the NPs. The Au/Ag-core/shell NPs were observed to be polydispersed in size, non-spherical, and contain off-centered Au-cores. Using the average NP dimensions determined from STEM analysis, SESSA spectral modeling indicated that washed Au/Ag-core shell NPs were stabilized with a 0.8 nm layer of sodium citrate and a 0.05 nm (one wash) or 0.025nm (two wash) layer of adventitious hydrocarbon, but didn’t fully account for the observed XPS signal from the Au core. This was addressed by a series of simulations and normalizations to account for contributions of NP non-sphericity and off-centered Au-cores. Both of these non-uniformities reduce the effective Ag-shell thickness, which effect the Au-core photoelectron intensity. The off-centered cores had the greatest impact for the particles in this study. When the contributions from the geometrical non-uniformities are included in the simulations, the SESSA generated elemental compositions that matched the XPS elemental compositions. This work demonstrates how spectral modeling software such as SESSA, when combined with experimental XPS and STEM measurements, advances the ability to quantitatively assess overlayer thicknesses for multilayer core-shell NPs and deal with complex, nonideal geometrical properties.

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“…Further alterations in silver nanoparticle dissolution due to modifications in their physicochemical properties have been shown to influence toxicological outcomes in vitro [54]. The addition of the biocorona may alter this dissolution resulting in the stabilization or destabilization of the nanoparticle structure.…”

Section: Challenges Presented By the Nanoparticle-biocoronamentioning

confidence: 99%

The biocorona: a challenge for the biomedical application of nanoparticles

Shannahan

2017

Nanotechnology Reviews

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Formation of the biocorona on the surface of nanoparticles is a significant obstacle for the development of safe and effective nanotechnologies, especially for nanoparticles with biomedical applications. Following introduction into a biological environment, nanoparticles are rapidly coated with biomolecules resulting in formation of the nanoparticle-biocorona. The addition of these biomolecules alters the nanoparticle’s physicochemical characteristics, functionality, biodistribution, and toxicity. To synthesize effective nanotherapeutics and to more fully understand possible toxicity following human exposures, it is necessary to elucidate these interactions between the nanoparticle and the biological media resulting in biocorona formation. A thorough understanding of the mechanisms by which the addition of the biocorona governs nanoparticle-cell interactions is also required. Through elucidating the formation and the biological impact of the biocorona, the field of nanotechnology can reach its full potential. This understanding of the biocorona will ultimately allow for more effective laboratory screening of nanoparticles and enhanced biomedical applications. The importance of the nanoparticle-biocorona has been appreciated for a decade; however, there remain numerous future directions for research which are necessary for study. This perspectives article will summarize the unique challenges presented by the nanoparticle-biocorona and avenues of future needed investigation.

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Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

Cited by 27 publications

References 81 publications

Cardiac Ischemia Reperfusion Injury Following Instillation of 20 nm Citrate-capped Nanosilver

Cardiac Ischemia Reperfusion Injury Following Instillation of 20 nm Citrate-capped Nanosilver

Quantifying the Impact of Nanoparticle Coatings and Nonuniformities on XPS Analysis: Gold/Silver Core–Shell Nanoparticles

The biocorona: a challenge for the biomedical application of nanoparticles

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