Abstract:The neuronal stomatin-like proteins UNC-1 and UNC-24 play important roles in the nervous system of Caenorhabditis elegans. These neuronal stomatin-like proteins are putative chaperone proteins that can modify volatile anesthetic sensitivity and disrupt coordinated locomotion. A suppressor of unc-1 and unc-24, named ssu-1(fc73) (for suppressor of stomatin uncoordination), suppresses three phenotypes of neuronal stomatin-like protein deficiency as follows: volatile anesthetic sensitivity, uncoordinated locomotio… Show more
“…To date, only the genes trx-1 (thioredoxin-1) and ssu-1 (alcohol sulfotransferase-1) have been reported to be expressed exclusively in ASJ neurons (Carroll et al 2006;MirandaVizuete et al 2006). TRX-1 belongs to the thioredoxin family of redox proteins, playing a role in regulating worm longevity and dauer formation (Miranda-Vizuete et al 2006;FierroGonzalez et al 2011a,b).…”
The identity of a given cell type is determined by the expression of a set of genes sharing common cis-regulatory motifs and being regulated by shared transcription factors. Here, we identify cis and trans regulatory elements that drive gene expression in the bilateral sensory neuron ASJ, located in the head of the nematode Caenorhabditis elegans. For this purpose, we have dissected the promoters of the only two genes so far reported to be exclusively expressed in ASJ, trx-1 and ssu-1. We hereby identify the ASJ motif, a functional cis-regulatory bipartite promoter region composed of two individual 6 bp elements separated by a 3 bp linker. The first element is a 6 bp CG-rich sequence that presumably binds the Sp family member zinc-finger transcription factor SPTF-1. Interestingly, within the C. elegans nervous system SPTF-1 is also found to be expressed only in ASJ neurons where it regulates expression of other genes in these neurons and ASJ cell fate. The second element of the bipartite motif is a 6 bp AT-rich sequence that is predicted to potentially bind a transcription factor of the homeobox family. Together, our findings identify a specific promoter signature and SPTF-1 as a transcription factor that functions as a terminal selector gene to regulate gene expression in C. elegans ASJ sensory neurons.
“…To date, only the genes trx-1 (thioredoxin-1) and ssu-1 (alcohol sulfotransferase-1) have been reported to be expressed exclusively in ASJ neurons (Carroll et al 2006;MirandaVizuete et al 2006). TRX-1 belongs to the thioredoxin family of redox proteins, playing a role in regulating worm longevity and dauer formation (Miranda-Vizuete et al 2006;FierroGonzalez et al 2011a,b).…”
The identity of a given cell type is determined by the expression of a set of genes sharing common cis-regulatory motifs and being regulated by shared transcription factors. Here, we identify cis and trans regulatory elements that drive gene expression in the bilateral sensory neuron ASJ, located in the head of the nematode Caenorhabditis elegans. For this purpose, we have dissected the promoters of the only two genes so far reported to be exclusively expressed in ASJ, trx-1 and ssu-1. We hereby identify the ASJ motif, a functional cis-regulatory bipartite promoter region composed of two individual 6 bp elements separated by a 3 bp linker. The first element is a 6 bp CG-rich sequence that presumably binds the Sp family member zinc-finger transcription factor SPTF-1. Interestingly, within the C. elegans nervous system SPTF-1 is also found to be expressed only in ASJ neurons where it regulates expression of other genes in these neurons and ASJ cell fate. The second element of the bipartite motif is a 6 bp AT-rich sequence that is predicted to potentially bind a transcription factor of the homeobox family. Together, our findings identify a specific promoter signature and SPTF-1 as a transcription factor that functions as a terminal selector gene to regulate gene expression in C. elegans ASJ sensory neurons.
“…S3). This gene might be required in C. elegans for the biosynthesis of a secreted compound(s) involved in dauer formation and locomotion (Carroll et al, 2006). We also identified at least five genes with similarity to genes encoding heparan sulfate (HS) sulfotransferases in the C. elegans genome.…”
Section: Identification Of Genes Involved In Sulfation In C Elegansmentioning
confidence: 89%
“…Numerous essential genes have been identified on the left arm of chromosome V, and grouped into complementation groups by deficiency mapping and complementation tests (Johnsen and Baillie, 1991;Stewart et al, 1991). Using the cold-sensitive lethality, we (Carroll et al, 2006) Genes were identified by keyword search in GenBank, PFAM and re-iterative BLAST searches.…”
Section: Pst-1 Is Required For Kal-1-induced Axonal Branching and Mismentioning
“…The gene is highly expressed in embryos, growing larvae and in dauer larvae. Expression of SSU-1 in the ASJ neurons (amphid sensory neurons) was demonstrated and the protein showed sulfotransferase activity in vitro (28). This protein may modify endocrine signals in the worm.…”
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