The PAPS transporter PST-1 is required for heparan sulfation and is essential for viability and neural development in<i>C. elegans</i>

Bhattacharya, Raja; Townley, Robert; Berry, Katherine L.; Bülow, Hannes E.

doi:10.1242/jcs.050732

Cited by 28 publications

(20 citation statements)

References 56 publications

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“…Heparan sulfate proteoglycans are a class of extracellular glycans of great molecular complexity that have been implicated in the function of KAL-1/anosmin-1 in cell culture, C. elegans , and humans (Soussi-Yanicostas et al, 1996; Soussi-Yanicostas et al, 1998; Bülow et al., 2002; Bülow and Hobert, 2004; Hudson et al, 2006; Bhattacharya et al, 2009; Tornberg et al., 2011; Tecle et al, 2013). We found that formation of HSN branches also required the heparan sulfate HS 6- O -sulfotransferase hst-6/HS6ST1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

The Adhesion Molecule KAL-1/anosmin-1 Regulates Neurite Branching through a SAX-7/L1CAM–EGL-15/FGFR Receptor Complex

et al. 2015

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Summary Neurite branching is essential for correct assembly of neural circuits, yet remains a poorly understood process. For example, the neural cell adhesion molecule KAL-1/anosmin-1, which is mutated in Kallmann Syndrome regulates neurite branching through mechanisms largely unknown. Here we show that KAL-1/anosmin-1 mediates neurite branching as an autocrine co-factor with EGL-17/FGF through a receptor complex consisting of the conserved cell adhesion molecule SAX-7/L1CAM and the fibroblast growth factor receptor EGL-15/FGFR. This protein complex, which appears conserved in humans, requires the immunoglobulin (Ig) domains of SAX-7/L1CAM and the FN(III) domains of KAL-1/anosmin-1 for formation in vitro as well as function in vivo. The kinase domain of the EGL-15/FGFR is required for branching, and genetic evidence suggests that ras-mediated signaling downstream of EGL-15/FGFR is necessary to effect branching. Our studies establish a molecular pathway that regulates neurite branching during development of the nervous system.

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Section: Resultsmentioning

confidence: 99%

The Adhesion Molecule KAL-1/anosmin-1 Regulates Neurite Branching through a SAX-7/L1CAM–EGL-15/FGFR Receptor Complex

et al. 2015

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“…In plants and animals, these reactions occur both in the cytosol and the Golgi lumen. Animal Golgi-resident PAPS transporters mediating the import of PAPS synthesized in the cytosol have been identified in Drosophila melanogaster (SLALOM; Lüders et al, 2003), human (PAPST1 and PAPST2; Kamiyama et al, 2003Kamiyama et al, , 2006, zebra fish (PAPST1; Clément et al, 2008), and Caenorhabditis elegans (PST-1; Bhattacharya et al, 2009). These transporters are involved in the sulfation of glycoconjugates, such as glycans, and the resulting sulfated molecules take part in the regulation of various developmental and intercellular signaling processes (Bishop et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis Thylakoid ADP/ATP Carrier TAAC Has an Additional Role in Supplying Plastidic Phosphoadenosine 5′-Phosphosulfate to the Cytosol

et al. 2012

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39-Phosphoadenosine 59-phosphosulfate (PAPS) is the high-energy sulfate donor for sulfation reactions. Plants produce some PAPS in the cytosol, but it is predominantly produced in plastids. Accordingly, PAPS has to be provided by plastids to serve as a substrate for sulfotransferase reactions in the cytosol and the Golgi apparatus. We present several lines of evidence that the recently described Arabidopsis thaliana thylakoid ADP/ATP carrier TAAC transports PAPS across the plastid envelope and thus fulfills an additional function of high physiological relevance. Transport studies using the recombinant protein revealed that it favors PAPS, 39-phosphoadenosine 59-phosphate, and ATP as substrates; thus, we named it PAPST1. The protein could be detected both in the plastid envelope membrane and in thylakoids, and it is present in plastids of autotrophic and heterotrophic tissues. TAAC/PAPST1 belongs to the mitochondrial carrier family in contrast with the known animal PAPS transporters, which are members of the nucleotide-sugar transporter family. The expression of the PAPST1 gene is regulated by the same MYB transcription factors also regulating the biosynthesis of sulfated secondary metabolites, glucosinolates. Molecular and physiological analyses of papst1 mutant plants indicate that PAPST1 is involved in several aspects of sulfur metabolism, including the biosynthesis of thiols, glucosinolates, and phytosulfokines.

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“…Previous studies have demonstrated that extracellular sulfated molecules play pivotal roles in nervous system development, gonadal cell migration, cuticle formation, and embryogenesis in C. elegans (37)(38)(39)(40)(41)(42). Most recently, pst-1 alleles were shown to be required for viability and neuronal development (43).…”

Section: Synthesis Of Extracellular Sulfated Molecules Requires Activmentioning

confidence: 99%

Two Golgi-resident 3′-Phosphoadenosine 5′-Phosphosulfate Transporters Play Distinct Roles in Heparan Sulfate Modifications and Embryonic and Larval Development in Caenorhabditis elegans

Dejima

Murata²,

Mizuguchi³

et al. 2010

Journal of Biological Chemistry

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Synthesis of extracellular sulfated molecules requires active 3-phosphoadenosine 5-phosphosulfate (PAPS). For sulfation to occur, PAPS must pass through the Golgi membrane, which is facilitated by Golgi-resident PAPS transporters. Caenorhabditis elegans PAPS transporters are encoded by two genes, pst-1 and pst-2. Using the yeast heterologous expression system, we characterized PST-1 and PST-2 as PAPS transporters. We created deletion mutants to study the importance of PAPS transporter activity. The pst-1 deletion mutant exhibited defects in cuticle formation, post-embryonic seam cell development, vulval morphogenesis, cell migration, and embryogenesis. The pst-2 mutant exhibited a wildtype phenotype. The defects observed in the pst-1 mutant could be rescued by transgenic expression of pst-1 and hPAPST1 but not pst-2 or hPAPST2. Moreover, the phenotype of a pst-1;pst-2 double mutant were similar to those of the pst-1 single mutant, except that larval cuticle formation was more severely defected. Disaccharide analysis revealed that heparan sulfate from these mutants was undersulfated. Gene expression reporter analysis revealed that these PAPS transporters exhibited different tissue distributions and subcellular localizations. These data suggest that pst-1 and pst-2 play different physiological roles in heparan sulfate modification and development.

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The PAPS transporter PST-1 is required for heparan sulfation and is essential for viability and neural development inC. elegans

Abstract: SummaryThe PAPS transporter PST-1 is required for heparan sulfation and is essential for viability and neural development in C. elegans

Cited by 28 publications

References 56 publications

The Adhesion Molecule KAL-1/anosmin-1 Regulates Neurite Branching through a SAX-7/L1CAM–EGL-15/FGFR Receptor Complex

The Adhesion Molecule KAL-1/anosmin-1 Regulates Neurite Branching through a SAX-7/L1CAM–EGL-15/FGFR Receptor Complex

The Arabidopsis Thylakoid ADP/ATP Carrier TAAC Has an Additional Role in Supplying Plastidic Phosphoadenosine 5′-Phosphosulfate to the Cytosol

Two Golgi-resident 3′-Phosphoadenosine 5′-Phosphosulfate Transporters Play Distinct Roles in Heparan Sulfate Modifications and Embryonic and Larval Development in Caenorhabditis elegans

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