Sulfated polysaccharides from Cyclocarya paliurus reduce H 2 O 2 -induced oxidative stress in RAW264.7 cells

Wang, Zhijun; Xie, Jianhua; Kan, Lijiao; Wang, Junqiao; Shen, Mingyue; Li, Wenjuan; Nie, Shaoping; Xie, Mingyong

doi:10.1016/j.ijbiomac.2015.06.031

Cited by 88 publications

(56 citation statements)

References 41 publications

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“…1) presented characteristic bands of carbohydrates, constituted by monomeric units linked by β-glycosidic bonds [27,32]. After sulfonation, bands characteristic of the insertion of sulfonate groups in the derivatized sample appeared in the regions of 1,240 and 810 cm -1 [20]. Similar observations have been reported by Vasconcelos et al [22] and Zhang et al [34] for sulfonated exopolysaccharides.…”

Section: Discussionsupporting

confidence: 85%

“…3C and 3D) can possibly be associated with a decrease in the hydrogen interactions along the macromolecule structure after substitutions of hydroxyls by the sulfonate groups. Wang et al [20] also observed structural morphological changes in a polysaccharide from Cyclocarya paliurus after sulfonation by the chlorosulfonic acid-pyridine method, compared to the unmodified native polysaccharide.…”

Section: Discussionmentioning

confidence: 96%

“…Bands in the 810 cm -1 region correspond to the C-O-S symmetrical vibration, associated with the C-O-SO 3 group, indicating that the native polysaccharide was sulfonylated [20,22,28,34,35]. The 13 C NMR spectra from LAS-N and LAS-S are presented in Fig.…”

Section: Chemical Characterizationmentioning

confidence: 99%

“…Among the chemical modifications described in the scientific literature, is sulfonation ( Fig. 1), which involves the substitution of hydroxyl groups by sulfonate groups (S + O 2 OH) [20,21]. The sulfonation reaction is usually performed using chlorosulfonic acid, anhydrous sulfuric acid and complexes of sulfur trioxide-pyridine as derivatizing agents [21].…”

Section: Introductionmentioning

confidence: 99%

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Sulfonation of (1→6)-β-D-Glucan (Lasiodiplodan) and Its Antioxidant and Antimicrobial Potential

Calegari¹,

Santos²,

Teixeira³

et al. 2017

JPP

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Abstract:The objective of this study was to investigate the sulfonation of (1→6)-β-D-glucan (lasiodiplodan) as a potentiating mechanism for biological functionalities. Lasiodiplodan was sulfonated by the chlorosulfonic acid-pyridine method. The modified exopolysaccharide was characterized by FT-IR and 13 C NMR spectroscopy, X-ray diffraction and SEM. Antioxidant activity was assessed by the methods of H 2 O 2 and OH radical removal and reducing power. Antimicrobial potential was evaluated by the broth-microdilution method. Sulfonation resulted in a derivative with DS of 0.24. FT-IR analysis indicated the introduction of sulfonyl groups in the macromolecule structure through specific bands in the regions of 1,240 cm -1 and 810 cm -1 . 13 C NMR analysis suggested that sulfonation occurred at carbon 2 of the glucose residue. Sulfonation led to morphological changes in the structure of the biopolymer resulting in a heterogeneous structure with the presence of fibrils. Derivatization promoted an increase in the antioxidant ability of the macromolecule, with a high OH removal potential (74.32%). Bacteriostatic activity against E. coli (Escherichia coli) and S. enterica (Salmonella enterica) typhimurium and fungicidal activity against C. albicans (Candida albicans) and C. tropicalis (Candida tropicalis) were found in the sulfonated sample. Sulfonation potentiated the antioxidant and antimicrobial activities of the biomacromolecule, suggesting that it is a potentiating mechanism of biological functions.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 96%

Section: Chemical Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sulfonation of (1→6)-β-D-Glucan (Lasiodiplodan) and Its Antioxidant and Antimicrobial Potential

Calegari¹,

Santos²,

Teixeira³

et al. 2017

JPP

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“…RAW264.7 cells were seeded in 96-wells plates cultivated as mentioned above, and the cell viability was measured using the CCK-8 assay53. After attachment culture for 4 h, the culture medium was removed and the cells were washed with phosphate buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice

Wang

Xie

Yang

et al. 2017

Sci Rep

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Natural polysaccharides and their modified derivatives are crucial supplements to the prevention of inflammation. This study aimed to evaluate the effect of sulfated modification on the anti-inflammatory and anti-oxidative activities of Cyclocarya paliurus polysaccharides (CP). A sulfated CP, S-CP1–4 was obtained using chlorosulfonic acid-pyridine method. The chemical components and FT-IR spectrum confirmed that sulfated group was synthesized to the polysaccharide chains successfully. S-CP1–4 was found to inhibit nitric oxide production, phagocytic activity and the release of interleukin (IL)-6 and IL-1β in lipopolysaccharide-treated macrophage cells, RAW 264.7. S-CP1–4 significantly decreased the secretion of IL-6 and TNF-α and the thymus and spleen indexes, and increased the production of IL-10 in lipopolysaccharide-treated mice. S-CP1–4 could better protect the liver by inhibiting the activities of alanine aminotransferase and aspartate aminotransferase, and malondialdehyde level while increasing the superoxide dismutase activity and total anti-oxidative capacity. These results suggested that S-CP1–4 may be an effective anti-inflammatory agent, and sulfated modification may be a reliable method for the development of food supplements.

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Engineered Sulfated Polysaccharides for Biomedical Applications

Arlov

Rütsche

Korayem

et al. 2021

Adv Funct Materials

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Sulfated polysaccharides are ubiquitous in living systems and have central roles in biological functions such as organism development, cell proliferation and differentiation, cellular communication, tissue homeostasis, and host defense. Engineered sulfated polysaccharides (ESPs) are structural derivatives not found in nature but generated through chemical and enzymatic modification of natural polysaccharides, as well as chemically synthesized oligo‐ and polysaccharides. ESPs exhibit novel and augmented biological properties compared with their unmodified counterparts, mainly through facilitating interactions with other macromolecules. These interactions are closely linked to their sulfation patterns and backbone structures, providing a means to fine‐tune biological properties and characterize structural–functional relationships by employing well‐characterized polysaccharides and strategies for regioselective modification. The following review provides a comprehensive overview of the synthesis and characterization of ESPs and of their biological properties. Through the pioneering research presented here, key emerging application areas for ESPs, which can lead to novel breakthroughs in biomedical research and clinical treatments, are highlighted.

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Sulfated polysaccharides from Cyclocarya paliurus reduce H 2 O 2 -induced oxidative stress in RAW264.7 cells

Cited by 88 publications

References 41 publications

Sulfonation of (1→6)-β-D-Glucan (Lasiodiplodan) and Its Antioxidant and Antimicrobial Potential

Sulfonation of (1→6)-β-D-Glucan (Lasiodiplodan) and Its Antioxidant and Antimicrobial Potential

Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice

Engineered Sulfated Polysaccharides for Biomedical Applications

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