Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice

Wang, Zhijun; Xie, Jianhua; Yang, Yujiao; Zhang, Fan; Wang, Shengnan; Wu, Ting; Shen, Mingyue; Xie, Mingyong

doi:10.1038/srep40402

Cited by 83 publications

(42 citation statements)

References 61 publications

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“…Because LPS induced RAW264.7 macrophage cells to polarize into M1 phenotype, our results showed that M1 phenotype macrophages (LPS treated macrophages) exhibited higher level of phagocytosis (Figure ). However, excess phagocytosis could cause inflammation and do harm to organs (Wang, Xie, et al, ). In our study, PSPPs treatment inhibited the excessive phagocytosis level as compared with LPS treated group ( p < .05).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

et al. 2018

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Immunomodulatory effects of water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP), and concentrated alkali-soluble polysaccharide (CASP) from purple sweet potato were evaluated by using lipopolysaccharide (LPS) treated RAW264.7 macrophage cell models. Results showed WSP, DASP, and CASP (100, 200, and 400 lg/mL) could suppress abnormal phagocytosis, overproduction of TNF-a and IL-6, and abnormal apoptosis caused by LPS. The high doses (200 and 400 lg/mL) of DASP and CASP could distinctly reduce excessive NO production as compared with LPS treatment group. In addition, 400 lg/mL of WSP, DASP, and CASP treatment groups exhibited dramatic decrease in the overexpression of IL-1b stimulated by LPS. Moreover, 200 and 400 lg/mL of WSP, DASP, and CASP markedly improved anti-inflammatory cytokine IL-10 as compared with LPS treatment group. Furthermore, 400 lg/mL of WSP and DASP treatments significantly increased the level of IL-10 in nonstimulated macrophages when compared with normal control group. Practical applicationsPurple sweet potato [Ipomoea batatas (L.) Lam] is one type of highly nutritional vegetable containing many vitamins, minerals, dietary fiber, anthocyanin, and polysaccharides. In addition, it is also one of health-promoting edible plant sources. Recently, polysaccharides have attracted extensive attentions due to their various biological activities. Our study reveals that polysaccharides extracted from purple sweet potato are effective in protecting macrophages damaged by lipopolysaccharide. Therefore, polysaccharides from purple sweet potato have anti-inflammatory efficacy.Thus, this study indicates the potential applications of purple sweet potato polysaccharides in functional food.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

et al. 2018

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“…Therefore, inflammatory molecules can be a potential thyroid cancer treatment target, while immunotherapy gradually become the focus in the treatment of thyroid cancer in recent years. As reported, qing qian liu polysaccharide has been proven to have the function of immune regulation and can inhibit the release of IL-6 and TNF-β by macrophages stimulated by LPS [16]. Cyclocarya paliurus polysaccharide is a kind of natural plant polysaccharide extracted from Cyclocarya paliurus.…”

Section: Discussionmentioning

confidence: 99%

Anticancer Effects of Cyclocarya paliurus Polysaccharide (CPP) on Thyroid Carcinoma In Vitro and In Vivo

Gan

et al. 2018

International Journal of Polymer Science

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Correspondence should be addressed to Ting Que; tque@outlook.com Zhiwei He and Fangfang Lv contributed equally to this work.In this study, we explored the role and mechanisms of Cyclocarya paliurus polysaccharide on cell apoptosis in thyroid cancer (TC) cells. The apoptosis of thyroid cancer cells in vitro and tumor tissues in vivo induced by Cyclocarya paliurus polysaccharide was determined by MTT assay and flow cytometric assay. The downstream molecules including phosphop-protein kinase B (p-Akt), Akt, B-cell lymphoma 2 (Bcl-2), and Bcl-2-associated X protein (Bax) in tumor tissue were evaluated by western blotting. MTT and flow cytometry assay in vitro revealed Cyclocarya paliurus polysaccharide-induced apoptosis of thyroid cancer cell line in a manner of time-dependent and dose-dependent. In vivo assay showed 50 mg/kg and 100 mg/kg Cyclocarya paliurus polysaccharide significantly suppressed the proliferation of thyroid cancer in mice. Western blotting showed downregulation of p-Akt, Akt, and Bcl-2 and upregulation of Bax. These results suggest that Cyclocarya paliurus polysaccharide may enhance thyroid cancer cell apoptosis by suppressing the activation of p-Akt, Akt, and Bcl-2 and activating Bax, which provide a novel use of CPP as a thyroid cancer treatment.

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“…The marine sourced sulfated polysaccharides have established anti-oxidative, antimicrobial, anticoagulant, and anticancer activities (Freudenberg et al, 2015; Song et al, 2015; Fernando et al, 2016; Prasedya et al, 2016; Wang et al, 2017). Biomaterial developed here capitalizes on the favorable properties of these individual components to regulate microenvironment thereby acting synergistically to promote healing.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Screening Identifies Synergistically Acting Natural Product Enhancing the Performance of Biomaterial Based Wound Healing

et al. 2017

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The potential of multifunctional wound heal biomaterial relies on the optimal content of therapeutic constituents as well as the desirable physical, chemical, and biological properties to accelerate the healing process. Formulating biomaterials such as amnion or collagen based scaffolds with natural products offer an affordable strategy to develop dressing material with high efficiency in healing wounds. Using image based phenotyping and quantification, we screened natural product derived bioactive compounds for modulators of types I and III collagen production from human foreskin derived fibroblast cells. The identified hit was then formulated with amnion to develop a biomaterial, and its biophysical properties, in vitro and in vivo effects were characterized. In addition, we performed functional profiling analyses by PCR array to understand the effect of individual components of these materials on various genes such as inflammatory mediators including chemokines and cytokines, growth factors, fibroblast stimulating markers for collagen secretion, matrix metalloproteinases, etc., associated with wound healing. FACS based cell cycle analyses were carried out to evaluate the potential of biomaterials for induction of proliferation of fibroblasts. Western blot analyses was done to examine the effect of biomaterial on collagen synthesis by cells and compared to cells grown in the presence of growth factors. This work demonstrated an uncomplicated way of identifying components that synergistically promote healing. Besides, we demonstrated that modulating local wound environment using biomaterials with bioactive compounds could enhance healing. This study finds that the developed biomaterials offer immense scope for healing wounds by means of their skin regenerative features such as anti-inflammatory, fibroblast stimulation for collagen secretion as well as inhibition of enzymes and markers impeding the healing, hydrodynamic properties complemented with other features including non-toxicity, biocompatibility, and safety.

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Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice

Cited by 83 publications

References 61 publications

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

Anticancer Effects of Cyclocarya paliurus Polysaccharide (CPP) on Thyroid Carcinoma In Vitro and In Vivo

Phenotypic Screening Identifies Synergistically Acting Natural Product Enhancing the Performance of Biomaterial Based Wound Healing

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