Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model

Raguraman, Vasantharaja; L, Stanley Abraham; Jyotsna, J.; Seedevi, Palaniappan; Gopal, S.; Ramanathan, T.; Kirubagaran, R.

doi:10.1016/j.carbpol.2018.09.056

Cited by 70 publications

(36 citation statements)

References 43 publications

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“…Pure gum exudate consists of complex hydrophilic polysaccharides, therefore, the purified polysaccharides were derivatized to their simple sugar moieties before detecting them in GC analysis as described earlier. 31 1 μL of the derivatized sample was loaded and the analysis was performed using JEOL GC MATE II HP 5 MS (5% phenyl-methylpolysiloxane) fitted with a Quadrupole double focusing Mass analyzer. The mobile phase used here was helium gas with a flow rate of 1mL/min.…”

Section: Characterization Of the Polysaccharidesmentioning

confidence: 99%

Extraction, Purification, and Characterization of Polysaccharides of Araucaria heterophylla L and Prosopis chilensis L and Utilization of Polysaccharides in Nanocarrier Synthesis

Samrot¹,

Kudaiyappan²,

Bisyarah³

et al. 2020

IJN

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Background: Plant gums consist of polysaccharides which can be used in the preparation of nanocarriers and provide a wide application in pharmaceutical applications including as drug delivery agents and the matrices for drug release. The objectives of the study were to collect plant gums from Araucaria heterophylla L and Prosopis chilensis L and to extract and characterize their polysaccharides. Then to utilize these plant gum-derived polysaccharides for the formulation of nanocarriers to use for drug loading and to examine their purpose in drug delivery in vitro. Methods: Plant gum was collected, polysaccharide was extracted, purified, characterized using UV-Vis, FTIR, TGA and GCMS and subjected to various bioactive studies. The purified polysaccharide was used for making curcumin-loaded nanocarriers using STMP (sodium trimetaphosphate). Bioactivities were performed on the crude, purified and drugloaded nanocarriers. These polysaccharide-based nanocarriers were characterized using UV-Vis spectrophotometer, FTIR, SEM, and AFM. Drug release kinetics were performed for the drug-loaded nanocarriers. Results: The presence of glucose, xylose and sucrose was studied from the UV-Vis and GCMS analysis. Purified polysaccharides of both the plants showed antioxidant activity and also antibacterial activity against Bacillus sp. Purified polysaccharides were used for nanocarrier synthesis, where the size and shape of the nanocarriers were studied using SEM analysis and AFM analysis. The size of the drug-loaded nanocarriers was found to be around 200 nm. The curcumin-loaded nanocarriers were releasing curcumin slow and steady. Conclusion: The extracted pure polysaccharide of A. heterophylla and P. chilensis acted as good antioxidants and showed antibacterial activity against Bacillus sp. These polysaccharides were fabricated into curcumin-loaded nanocarriers whose size was below 200 nm. Both the drug-loaded nanocarriers synthesized using A. heterophylla and P. chilensis showed antibacterial activity with a steady drug release profile. Hence, these natural exudates can serve as biodegradable nanocarriers in drug delivery.

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Section: Characterization Of the Polysaccharidesmentioning

confidence: 99%

Extraction, Purification, and Characterization of Polysaccharides of Araucaria heterophylla L and Prosopis chilensis L and Utilization of Polysaccharides in Nanocarrier Synthesis

Samrot¹,

Kudaiyappan²,

Bisyarah³

et al. 2020

IJN

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“…The ABTS radical scavenging activity of sulfated polysaccharides from Sargassum tenerrimum was lower than 50% at the highest concentration (125 µg/mL). [29] Furthermore, Figure 5 of the polysaccharides. PGCL and PULV scavenged DPPH radicals; however, the activity of both polysaccharides was lower than 50% at the highest concentration (333.33 µg/mL).…”

Section: Radical Scavenging and Metal Chelating Activitiesmentioning

confidence: 99%

“…The DPPH radical scavenging activity of PGCL and PULV is significantly different from the activity of sulfated polysaccharides isolated from S. tenerrimum. [29] The OH radical scavenging activity of PGCL and PULV is shown in Figure 5(c). Both polysaccharides scavenged OH radicals in a dose-dependent manner (25-100 µg/mL).…”

Section: Radical Scavenging and Metal Chelating Activitiesmentioning

confidence: 99%

Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities

Olasehinde

Mabinya

Olaniran

et al. 2019

International Journal of Food Properties

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This study was designed to evaluate the antioxidant and cholinesterase inhibitory activities of sulfated polysaccharides from Gracilaria gracilis (PGCL) and Ulva lactuca (PULV). PGCL and PULV were extracted and characterized with Scanning electron microscope-energy dispersive x-ray (SEM-EDX), Fourier transform infrared spectroscopy (FTIR) and Gas chromatography-mass spectroscopy (GC-MS). The radical (2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate [ABTS] 2,2-diphenyl-1-picrylhydrazyl [DPPH], and hydroxyl [OH]) scavenging and metal chelating activities as well as their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were also determined. SEM-EDX analysis revealed the presence of elements such as carbon, magnesium, calcium, phosphorus, and sulfur. The FTIR analysis also confirmed the presence of sulfate group in the polysaccharide samples. Some sugars such as glucose, galactose, arabinose, xylose, rhamnose, and allose were also identified using GC-MS. PGCL and PULV scavenged ABTS, DPPH and OH radicals in a dose-dependent manner. Moreover, PULV exhibited higher scavenging activity compared to PGCL. PGCL and PULV also reduced the activity of AChE (IC 50 = 132.73 and 106.93 µg/mL) and BChE (IC 50 = 124.93 and 93.45 µg/mL) respectively. PGCL and PULV exhibited antioxidant activity and possess cholinesterase inhibitory activity, hence these polysaccharides could be explored as ingredients for the development of functional foods with neuroprotective potentials.

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“…The aqueous polysaccharide was then lyophilized and stored in airtight containers.2.4. Characterization of polysaccharideThe extracted polysaccharide was subjected for UV-Vis Spectroscopic analysis, Fourier Transform Infrared Spectroscopic analysis (Shimadzu, Japan),phenol sulphuric acid assay(Mahendran et al 2008, Albalasmeh et al 2013, TGA analysis and TLC (having butanol : acetic acid : water (2:1:1) as mobile phase) and GC -MS (JMS-TQ4000GC GC Triple Quadrupole Mass Spectrometer) analysis after derivatization to identify monosaccharides(Gallina et al 2004, Raguraman et al 2019.…”

mentioning

confidence: 99%

Purification, characterization and exploitation of Azadirachta indica gum for the production of drug loaded nanocarrier

Samrot

Angalene

Roshini

et al. 2020

Mater. Res. Express

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In this study, gum polysaccharide of Azadirachta indica was extracted and purified. The obtained polysaccharide was subjected to TLC chromatography, spectroscopic analysis, thermogravimetric analysis and GC-MS analysis. The polysaccharide was found to have Glucose, Idosan, Allose, Galactose, Ribose and Xylose. The polysaccharide was not having antibacterial activity but possessed good antioxidant and anticancer activity. The extracted polysaccharide was further carboxymethylated and used for the synthesis of nanocarrier to carry anticancer drug, curcumin. Size of the drug unloaded nanocarrier were found to be size below 40 nm, whereas the drug loaded nanocarriers were around 50 to 70 nm. The nanocarriers were studied for cytotoxicity against MCF7 cancer cell line and found to be effective.

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Sulfated polysaccharide from Sargassum tenerrimum attenuates oxidative stress induced reactive oxygen species production in in vitro and in zebrafish model

Cited by 70 publications

References 43 publications

<p>Extraction, Purification, and Characterization of Polysaccharides of <em>Araucaria heterophylla</em> L and <em>Prosopis chilensis</em> L and Utilization of Polysaccharides in Nanocarrier Synthesis</p>

<p>Extraction, Purification, and Characterization of Polysaccharides of <em>Araucaria heterophylla</em> L and <em>Prosopis chilensis</em> L and Utilization of Polysaccharides in Nanocarrier Synthesis</p>

Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities

Purification, characterization and exploitation of Azadirachta indica gum for the production of drug loaded nanocarrier

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