Suitability of the alkalistable carbonic anhydrase from a polyextremophilic bacterium Aeribacillus pallidus TSHB1 in biomimetic carbon sequestration

Bose, Himadri; Satyanarayana, T.

doi:10.1007/s00449-016-1627-4

Cited by 21 publications

(12 citation statements)

References 49 publications

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“…The sequence of 16S rRNA gene was not included in tree because of a short sequence (432 bp) available in GenBank. The alkalistable carbonic anhydrase producing Aeribacillus pallidus TSHB1 has been isolated from Choti Anhoni hot spring of Pipariya, Madhya Pradesh, India 51 . These findings reveal that Aeribacillus is widely distributed in different habitat.…”

Section: Journal Of Pure and Applied Microbiologymentioning

confidence: 99%

Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Neelam¹,

Agrawal²,

Tomer³

et al. 2018

J Pure Appl Microbiol

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Microorganisms inhabit extreme environments are novel in terms of phylogeny and also valuable source of extracellular hydrolytic enzymes. The present study focused on isolation and characterization of moderately thermophilic heterotrophic bacteria from Thar Desert using culture dependent method and assessment of their potential for production of industrially important enzymes. Screening of bacteria from sand dune samples collected from arid region of India led to isolation of total eight moderately thermophilic bacterial strains. All strains were characterized morphologically, biochemically and 16S rRNA gene sequencing analysis. The phylogenetic analysis of strains revealed that these strains affiliated to genera Bacillus (5 strains), Geobacillus (2 strains) and Aeribacillus (1 strain), which were able to grow between 25-60 °C. The present study documented the occurrence of species Geobacillus thermodenitificans, Bacillus paralicheniformis and Aeribacillus pallidus in sand dunes of Thar Desert, India. These moderately thermophilic strains showed an ability to produce one or more extracellular enzymes like amylase, protease, lipase and asparaginase. Thus, the strains isolated from Thar Desert could be potential candidates for industrial applications. The present study showed that bacterial strains exist in the same ecological niche might have ability to produce distinct enzymes.

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Section: Journal Of Pure and Applied Microbiologymentioning

confidence: 99%

Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Neelam¹,

Agrawal²,

Tomer³

et al. 2018

J Pure Appl Microbiol

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“…BhCA did not get denatured in the presence of EDTA (Faridi and Satyanarayana, 2016b ). Bose and Satyanarayana ( 2016 ) reported a moderately thermostable and alkalistable γ–CA from Aeribacillus pallidus , a polyextremophilic bacterium. Both ApCA and BhCA were stable in presence presence of bicarbonate (0.1M).…”

Section: Carbon Capture and Storage (Ccs)mentioning

confidence: 99%

“…Another thermo-alkali-stable γ–CA (ApCA) from polyextremophilic bacterium A. pallidus was used for biomineralization and well faceted rhombohedral calcite crystals were observed when viewed under scanning electron microscopy (SEM; Figure 1 ). The partially purified enzyme caused precipitation of 42.5 mg

mg −1 protein (Bose and Satyanarayana, 2016 ). Optimization of the process parameters led to an increase in carbonate precipitation up to 200 mg

mg −1 protein (Bose and Satyanarayana, 2017 ).…”

Section: Carbon Capture and Storage (Ccs)mentioning

confidence: 99%

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Microbial Carbonic Anhydrases in Biomimetic Carbon Sequestration for Mitigating Global Warming: Prospects and Perspectives

Bose

Satyanarayana

2017

Front. Microbiol.

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All the leading cities in the world are slowly becoming inhospitable for human life with global warming playing havoc with the living conditions. Biomineralization of carbon dioxide using carbonic anhydrase (CA) is one of the most economical methods for mitigating global warming. The burning of fossil fuels results in the emission of large quantities of flue gas. The temperature of flue gas is quite high. Alkaline conditions are necessary for CaCO3 precipitation in the mineralization process. In order to use CAs for biomimetic carbon sequestration, thermo-alkali-stable CAs are, therefore, essential. CAs must be stable in the presence of various flue gas contaminants too. The extreme environments on earth harbor a variety of polyextremophilic microbes that are rich sources of thermo-alkali-stable CAs. CAs are the fastest among the known enzymes, which are of six basic types with no apparent sequence homology, thus represent an elegant example of convergent evolution. The current review focuses on the utility of thermo-alkali-stable CAs in biomineralization based strategies. A variety of roles that CAs play in various living organisms, the use of CA inhibitors as drug targets and strategies for overproduction of CAs to meet the demand are also briefly discussed.

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“…The economic feasibility is also essential for the commercialization of technology. Bose and Satyanarayana () demonstrated an increase of 3.7‐fold in the production of moderated alkali stable (pH 9.0) and hyperthermostable (60°C) CA for calcite production (Bose and Satyanarayana ).…”

Section: Development In Ca‐based Mineralization and Phylogenetic Distmentioning

confidence: 99%

Trends, application and future prospectives of microbial carbonic anhydrase mediated carbonation process for CCUS

2017

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Summary Growing industrialization and the desire for a better economy in countries has accelerated the emission of greenhouse gases (GHGs), by more than the buffering capacity of the earth's atmosphere. Among the various GHGs, carbon dioxide occupies the first position in the anthroposphere and has detrimental effects on the ecosystem. For decarbonization, several non‐biological methods of carbon capture, utilization and storage (CCUS) have been in use for the past few decades, but they are suffering from narrow applicability. Recently, CO2 emission and its disposal related problems have encouraged the implementation of bioprocessing to achieve a zero waste economy for a sustainable environment. Microbial carbonic anhydrase (CA) catalyses reversible CO2 hydration and forms metal carbonates that mimic the natural phenomenon of weathering/carbonation and is gaining merit for CCUS. Thus, the diversity and specificity of CAs from different micro‐organisms could be explored for CCUS. In the literature, more than 50 different microbial CAs have been explored for mineral carbonation. Further, microbial CAs can be engineered for the mineral carbonation process to develop new technology. CA driven carbonation is encouraging due to its large storage capacity and favourable chemistry, allowing site‐specific sequestration and reusable product formation for other industries. Moreover, carbonation based CCUS holds five‐fold more sequestration capacity over the next 100 years. Thus, it is an eco‐friendly, feasible, viable option and believed to be the impending technology for CCUS. Here, we attempt to examine the distribution of various types of microbial CAs with their potential applications and future direction for carbon capture. Although there are few key challenges in bio‐based technology, they need to be addressed in order to commercialize the technology.

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Suitability of the alkalistable carbonic anhydrase from a polyextremophilic bacterium Aeribacillus pallidus TSHB1 in biomimetic carbon sequestration

Cited by 21 publications

References 49 publications

Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Microbial Carbonic Anhydrases in Biomimetic Carbon Sequestration for Mitigating Global Warming: Prospects and Perspectives

Trends, application and future prospectives of microbial carbonic anhydrase mediated carbonation process for CCUS

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