Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Neelam, Deepesh Kumar; Agrawal, Akhil; Tomer, Anuj Kumar; Dadheech, Pawan K.

doi:10.22207/jpam.12.4.24

Cited by 3 publications

(1 citation statement)

References 49 publications

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“…Obeidat (2017) reports that B. paralicheniformis isolated from Dead Sea black mud was classified as an extremely halotolerant and exhibited esterase activity on API ZYM tested. Another study concluded that Bacillus paralicheniformis isolated from the dunes of the Thar Desert in India as a moderately thermophilic strain and showed an ability to produce extracellular lipase (Neelam et al, 2018).…”

Section: Dna-fingerprint Of Lpmhb In Genus Bacillusmentioning

confidence: 99%

Genome, physicochemical properties and esterase gene cloning of novel lentibacillus and halobacillus species

Booncharoen

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A total of 831 moderately halophilic bacteria isolated from various samples in Thailand were screened for their lipolytic activity. Three hundred and forty-nine isolates showed activity on agar plate while 322 isolates exhibited in broth. The grouping and identification of 322 isolates based on DNA-fingerprinting using (GTG)5+ERIC2 primer and 16S rRNA gene sequence, 180 DNA patterns could be generated. The result indicated that the representative isolates belonged to genera Bacillus, Halobacillus, Lentibacillus, Marinobacter, Thallossobacillus, and Virgibacillus. On the basis of polyphasic taxonomic study and genome analysis, a selected moderately halophilic strain SSKP1-9T, Gram-staining-positive, aerobic, endospore-forming, rod-shaped was closely related to Lentibacillus juripiscarius TISTR 1535T and L. halophilus TISTR 1549T, 98.7 and 97.2%, respectively based on 16S rRNA gene sequence similarity. On the basis of these results, the strain represents a novel species of the genus Lentibacillus, for which the name Lentibacillus lipolyticus is proposed (SSKP1-9T=JCM 32625T=TISTR 2597T). A Gram-staining-positive, aerobic, endospore-forming, rod-shaped, strain SKP4-6T was closely related to�Halobacillus salinus JCM 11546T, H. locisalis KCTC 3788T and H. yeomjeoni KCTC 3957T, with 98.6% respectively, based on 16S rRNA gene sequence similarity. On the basis of these results taxonomy and whole-genome analysis, strain SKP4-6T represents a novel species of the genus Halobacillus, for which the name Halobacillus fulvus is proposed (SKP4-6T =JCM 32624T=TISTR 2595T). Based on whole-genome analysis, strain SSKP1-9T and SKP4-6T contained protein-encoding esterase for 8 and 5 genes, respectively. The genes were then cloned into the pSaltExSePR5 plasmid and transferred to Bacillus subtilis WB800. Two recombinant, >APNFEMBD_02578-C112-40 from strain SSKP1-9T and >NKILIEJB_01195-C9-35 from strain SKP4-6T showed the highest esterase activity and were therefore studied. These two genes identified major matches with closely related to a member of family acyl-CoA thioesterase and the alpha/beta hydrolase fold founded in genus Lentibacillus and Halobacillus, respectively. Of the two recombinant enzymes, the first one has a molecular weight of 16.2 kDa, and the other was 27.7 kDa, identical to the predicted protein size from the amino acid sequence. Two recombinant enzymes reached the maximum activity at 15-30% NaCl, and the highest activity was shown at 22 % NaCl. The recombinant >APNFEMBD_02578-C112-40 displayed the activity around 25-60 ?C, and the maximum activity was observed at 40-55 ?C while >NKILIEJB_01195-C9-35 had optimal activity at 55 ?C and lost its activity rapidly above 65 ?C of the remaining activity. They stable in NaCl concentration above 17% (w/v) after incubated at 55 ?C for 1 hr. Two enzymes showed good stability and remained activity about 80% in a temperature range of 5-45 ?C after 1 hr of incubation at 50 ?C with or without NaCl. Theses enzymes reached high activity over a wide range of pH from 6.0 to 9.0, recording maximal activity at pH 7.0-8.0 and displayed excellent stable in with or without NaCl at various pH ranges (pH 6.0 to 9.0). Besides, the two recombinant enzymes were found to have higher esterase activity against p-nitrophenyl butyrate (C4:0) comparing to other esters substrate. Based on finding results, two recombinant enzymes were classified as halophilic and thermophilic esterase�

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Section: Dna-fingerprint Of Lpmhb In Genus Bacillusmentioning

confidence: 99%

Genome, physicochemical properties and esterase gene cloning of novel lentibacillus and halobacillus species

Booncharoen

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Microbial diversity and ecology of saline environments from India

Thombre

Mangrola

2022

Microbial Diversity in Hotspots

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Screening of antibacterial activities of Bacillus spp. isolated from the Parangkusumo coastal sand dunes, Indonesia

Geraldi

Famunghui

Abigail

et al. 2022

BIOI

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Background: The emergence of multidrug-resistant bacteria because of poor understanding of the issue and the misuse of antibiotics has become global health concern. Therefore, the discovery of novel antibacterial drugs is urgently needed. New antibacterial compounds may be found in the Bacillus species, which are abundant in sand dune ecosystems. Herein, we examined samples from the Parangkusumo coastal sand dunes in Indonesia.Methods: Samples were collected from three areas in the sand dunes (the area closest to the sea, the core area of sand dunes, and the area farthest from the sea). The samples were inoculated on Luria Bertani agar. Morphological and molecular identification was performed on the basis of 16S rRNA. The samples’ antimicrobial activity was evaluated with the disc diffusion method and compared with that of opportunistic pathogenic bacteria.Results: Five species of Bacillus were successfully isolated from the Parangkusumo coastal sand dunes. To our knowledge, this is the first report of the isolation of Bacillus aryabhattai in Indonesia. All samples showed antimicrobial activity against pathogenic bacteria. B. velezensis and B. subtilis showed antibacterial activity against Gram-positive bacteria, whereas B. aryabhattai and B. megaterium showed antibacterial activity against Gram-negative bacteria, and B. spizizenii showed antibacterial activity toward Gram-positive and Gram-negative bacteria.Conclusion: Five Bacillus species were successfully isolated from the Parangkusumo coastal sand dunes, Indonesia, and all samples showed antimicrobial activity toward opportunistic pathogenic bacteria. The crude antimicrobial compounds from B. megaterium, B. aryabhattai, B. subtilis, and B. spizizenii showed the highest growth-inhibition activity against E. coli, P. aeruginosa, B. cereus, and S. aureus, respectively.

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Characterization, Phylogenetic Analysis and Potential Applications of Heterotrophic Bacteria Inhabit Sand Dunes of Thar Desert, India

Cited by 3 publications

References 49 publications

Genome, physicochemical properties and esterase gene cloning of novel lentibacillus and halobacillus species

Genome, physicochemical properties and esterase gene cloning of novel lentibacillus and halobacillus species

Microbial diversity and ecology of saline environments from India

Screening of antibacterial activities of Bacillus spp. isolated from the Parangkusumo coastal sand dunes, Indonesia

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