Suitability of lysosomal membrane stability in Eisenia fetida as biomarker of soil copper contamination

Rocco, A.; Scott-Fordsmand, Janeck J.; Maisto, Giulia; Manzo, Sonia; Salluzzo, Antonio; Jensen, John

doi:10.1016/j.ecoenv.2011.01.013

Cited by 23 publications

(8 citation statements)

References 37 publications

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“…In the majority of studies dealing with the Cu toxicity, soils are generally spiked with the soluble CuCl 2 salts (Amorim et al, 2005;Criel et al, 2008;Menezes-Oliveira et al, 2011;Rocco et al, 2011). Comparisons between the exposure of invertebrates to Cu salt and Cu (nano)particles have only recently started to be investigated .…”

Section: Discussionmentioning

confidence: 99%

“…Copper and its toxicity to soil invertebrates have been widely investigated, due both to its common use in biocides/pesticides and its direct toxic action or food-chain accumulation in living organisms Amorim et al, 2005;Criel et al, 2008;Menezes-Oliveira et al, 2011;Rocco et al, 2011;ScottFordsmand et al, 2008). Soil physicochemical properties can be used to predict toxicity using regression analysis (Criel et al, 2008;Frouz et al, 2005;Van Gestel et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Soil invertebrates play an important role in soil structuring, aeration, soil organic matter decomposition, and nutrient cycling processes (Jänsch et al, 2005;Rocco et al, 2011;van Gestel et al, 2011). Enchytraeids (Oligochaeta) play this role in many soils worldwide (Jänsch et al, 2005) and are recommended as a test species within the OECD ecotoxicological testing programme (ISO, 2005;OECD, 2004), which among other factors enable studies to be compared worldwide.…”

Section: Introductionmentioning

confidence: 99%

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Response of Enchytraeus crypticus worms to high metal levels in tropical soils polluted by copper smelting

Konecny

Ettler

Kristiansen

et al. 2014

Journal of Geochemical Exploration

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Tropical soils from the vicinity of the Mufulira copper smelter (Zambian Copperbelt) were found to contain high levels of metals, particularly in the upper parts of soil horizons. Metal concentrations varied within the ranges: 37-8980 mg Cu/kg, 3-46 mg Co/kg, b 2.5-42 mg Pb/kg, and 16-83 mg Zn/kg. The EDTA extractions indicated that a considerable percentage of the metals were bound in the "labile" fractions of the soil (up to 55% of total Cu concentration, 36% Co, 41% Pb, 30% Zn). The mineralogical investigation of the heavy mineral fraction of the most contaminated soil showed that the Cu is mainly present as Cu-(Fe) sulphides (chalcocite, chalcopyrite), metallic Cu, complex Cu-(Fe) oxides, and Cu-bearing sulphates. To determine the toxicity of these soils, we adopted a standardized OECD reproduction test using Enchytraeus crypticus. The number of reproduced enchytraeids correlated negatively with total Cu and Co concentrations (r = −0.97 and −0.94 at p b 0.001, respectively), and EDTA-extractable Cu (r = −0.89, p b 0.001); whereas no effects of the soil properties (pH, CEC, C org ) were observed. No reproduction was possible in soils with Cu levels N5000 mg/kg. Median effect concentration (EC50) was calculated for total Cu concentration, and corresponded to 351 mg/kg.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Response of Enchytraeus crypticus worms to high metal levels in tropical soils polluted by copper smelting

Konecny

Ettler

Kristiansen

et al. 2014

Journal of Geochemical Exploration

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“…In particular, lysosomal membrane destabilization (assessed by lysosomal enzyme latency in frozen tissue sections or lysosomal dye retention in circulating cells) is one on the most commonly used biomarkers in invertebrates in environmental biomonitoring. It is a general biomarker of effect very sensitive to both inorganic and organic toxic chemicals in a number of sentinel species [35,36]. The reduction of lysosomal membrane stability is often associated with an increase of the lysosome/cell volume ratio [37], which is in turn indicative of a not physiological level of cell catabolism.…”

Section: Pollution Biomarkers In Environmental Biomonitoringmentioning

confidence: 99%

Pollution Biomarkers in Environmental and Human Biomonitoring

Lionetto¹,

Caricato²,

Giordano³

2019

TOBIOMJ

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Environmental pollutants generate harmful conditions for living organisms, including humans. This accounts for the growing interest to early warning tools for detection of adverse biological responses to pollutants in both humans and wildlife. Molecular and cellular biomarkers of pollution meet this requirement. A pollution biomarker is defined as an alteration in a biological response occurring at molecular, cellular or physiological levels which can be related to exposure to or toxic effects of environmental chemicals.Pollution biomarkers have known a growing development in human and environmental biomonitoring representing a valuable tool for early pollutant exposure detection or early effect assessment (exposure/effect biomarkers).The review discusses the recent developments in the use of pollution biomarker in human and environmental biomonitoring and analyzes future perspectives in the application of this tool such as their potentiality for bridging human and environmental issued studies.

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“…Control group of animals were maintained in mentioned conditions during 7 or 14 days of periods (7 days of control group and 14 days of control group). Copper oxychloride was applied to the soil in a single application by mixing 500 g of artificial soil with 250 ml of copper oxychloride solution in a concentration corresponding to 350 mg of Cu to the kg of artificial soil [17]. Experimental group animals were exposed to copper oxychloride during 7 or 14 days of periods (350 mg/kg/art.…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure

Kılıç¹,

Kılıç²

2019

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology

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Morphological alterations in Eisenia fetida coelomocytes are recommended biomarkers for soil contamination surveys. Copper oxychloride is a widely used agrochemical agent that causes excessive amounts of Cu accumulation in soils. The present study is a light and electron microscopic investigation of the morphological alterations in coelomocytes of E. fetida exposed to copper oxychloride. Exposure concentration was 350 mg/kg of artificial soil and exposure duration was 7 or 14 days. Colelomocytes were extrused into an ethanol and guaicol glyceryl (GGE) containing modified Hanks' balanced solution (M-HBSS-GGE) by a simple low vacuum holding device and processed for light and scanning electron microscopy. Lipid peroxidation (LP) in the earthworms was also measured to evaluate oxidative stress (OS). Swelling and vesicular deformation were the early signs of toxicity in eleocytes after 7 days. After 14 days, complete loss of vesicles and spongy surface were observed in eleocytes. Granulocytes showed more dramatical changes. Nucleus fragmentation and membrane rupture were observed after 14 days. Increased LP in the earthworms was accompanying these changes. As a result, along with biochemical markers such as LP, light and scanning electron microscopic changes in E.fetida eleocytes and granulocytes can be considered as valuable biomarkers of copper oxychloride toxicity. Evaluation of those markers in the future studies can constitute an important early warning in terms of soil accumulation.

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Suitability of lysosomal membrane stability in Eisenia fetida as biomarker of soil copper contamination

Cited by 23 publications

References 37 publications

Response of Enchytraeus crypticus worms to high metal levels in tropical soils polluted by copper smelting

Response of Enchytraeus crypticus worms to high metal levels in tropical soils polluted by copper smelting

Pollution Biomarkers in Environmental and Human Biomonitoring

Light and Scanning-Electron Microscopic Study of Eisenia fetida Coelomocytes after Copper Oxychloride Exposure

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