Suitability of hybrid mackerel (Scomber australasicus × S. japonicus) with germ cell-less sterile gonads as a recipient for transplantation of bluefin tuna germ cells

Kawamura, Wataru; Tani, Reoto; Yahagi, Hana; Kamio, Shigeharu; Morita, Tetsuro; Takeuchi, Yutaka; Yazawa, Ryosuke; Yoshizaki, Goro

doi:10.1016/j.ygcen.2020.113525

Cited by 22 publications

(26 citation statements)

References 50 publications

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“…Strain specific differences or different age of the assessed fish can probably play an important role in gonadal phenotype of hybrids. Similar variance in testicular phenotype was described in mackerel hybrid of Scomber australasicus x S. japonicus , when part of the hybrid males could proceed through spermatogenesis while the second phenotype was germ cell less (Kawamura et al, 2020). Consequently, semi fertility of the hybrid could interfere with the fertilization since endo- and exogenous gametes are in the competition for the ova.…”

Section: Discussionsupporting

confidence: 70%

Who is the best surrogate for germ stem cell transplantation in fish?

Franěk

Cheng

Fučíková

et al. 2021

Preprint

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Surrogate reproduction technology in fish has potential for aquaculture as well as endangered species preservation and propagation. Species with some unfavourable biological characteristics for culturing such as a late maturation or a large body size are ideal candidates for surrogate reproduction using smaller and faster-maturing host. One of the general prerequisites for the successful surrogacy and the pure donor-derived gamete production is the sterility of the host. Various sterilization methods have been developed and used in fish surrogacy; however, a direct comparison of available methods is missing. Such a knowledge gap hinders choice for the surrogate in various fish species, including those in high commercial demand such as tuna or sturgeons, where is a particular limitation from the point of the live material availability and difficulty to perform a high throughput assessment of different surrogates. Yet, large sturgeons or tuna species are one of the most prominent candidates for surrogacy. Zebrafish was utilized in this study as a model species to answer whether and to which extent different sterilization strategies can affect the surrogacy. Germ cell-depleted recipients (produced using knockdown of dead end gene), triploid recipients, and zebrafish x pearl danio hybrid recipients were tested as they represent the most frequently used types of surrogates. Spermatogonia isolated from vas::EGFP transgenic strain were intraperitoneally transplanted into swim-up 5-day old zebrafish. Transplantation success, survival, gonadal development, and reproductive output of the fish was analyzed. Germ cell-depleted recipients with empty gonads were identified as the most convenient among tested sterilization methods considering surrogacy induction success and reproductive output. The present study stands as significant aid for selecting suitable surrogates in various fish species.

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Section: Discussionsupporting

confidence: 70%

Who is the best surrogate for germ stem cell transplantation in fish?

Franěk

Cheng

Fučíková

et al. 2021

Preprint

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“…Germ cell-less phenotype is not common in hybrid gonads, although some cases have been described in hybrid fish [49,50]. It has been proposed that the absence of germ cells in these cases may be owing to cell-autonomous proliferative defects of hybrid primordial germ cells [50].…”

Section: Discussionmentioning

confidence: 99%

Interaction between sex-determining genes from two species: clues fromXenopushybrids

Roco

Ruiz-García

Bullejos

2021

Phil. Trans. R. Soc. B

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Hybrids provide an interesting model to study the evolution of sex-determining genes and sex chromosome systems as they offer the opportunity to see how independently evolving sex-determining pathways interact in vivo . In this context, the genus Xenopus represents a stimulating model, since species with non-homologous sex chromosomes and different sex-determining genes have been identified. In addition, the possibility of interspecies breeding is favoured in this group, which arose by alloploidization events, with species ploidy ranging from 2 n = 2 x = 20 in X. tropicalis (the only diploid representative of the genus) to 2 n = 12 x = 108 in X. ruwenzoriensis . To study how two sex-determining genes interact in vivo , X. laevis × X. tropicali s hybrids were produced. Gonadal differentiation in these hybrids revealed that the dm-w gene is dominant over X. tropicalis male-determining sex chromosomes (Y or Z), even though the Y chromosome is dominant in X. tropicalis (Y > W>Z). In the absence of the dm-w gene (the Z chromosome from X. laevis is present), the W chromosome from X. tropicalis is able to trigger ovarian development. Testicular differentiation will take place in the absence of W chromosomes from any of the parental species. The dominance/recessivity relationships between these sex-determining loci in the context of either parental genome remains unknown. This article is part of the theme issue ‘Challenging the paradigm in sex chromosome evolution: empirical and theoretical insights with a focus on vertebrates (Part II)’.

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“…Additionally, triploidisation or similar strategies are only practical in diploid species, as for example individuals with 1.5 fold increment of chromosome sets of the tetraploid species Siberian sturgeon ( Acipenser baerii ) are fertile ( Havelka et al, 2014 ). Interspecies hybrids are also typically sterile and have been used as germ cell recipients in various species ( Kawamura et al, 2020 ; Piva et al, 2018 ; Wong et al, 2011 ; Yamaha et al, 2003 ; Yoshikawa et al, 2018 ). However, this method will be limited to certain species which have available sterile hybrids such as blue drum ( Yoshikawa et al, 2018 ) and mackerel ( Kawamura et al, 2020 ).…”

Section: Surrogate Broodstock Technologymentioning

confidence: 99%

“…Interspecies hybrids are also typically sterile and have been used as germ cell recipients in various species ( Kawamura et al, 2020 ; Piva et al, 2018 ; Wong et al, 2011 ; Yamaha et al, 2003 ; Yoshikawa et al, 2018 ). However, this method will be limited to certain species which have available sterile hybrids such as blue drum ( Yoshikawa et al, 2018 ) and mackerel ( Kawamura et al, 2020 ). Therefore, both triploidisation and hybridisation have limited utility for achieving sterile surrogate hosts.…”

Section: Surrogate Broodstock Technologymentioning

confidence: 99%

Surrogate broodstock to enhance biotechnology research and applications in aquaculture

Jin

Robledo

Hickey

et al. 2021

Biotechnology Advances

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Aquaculture is playing an increasingly important role in meeting global demands for seafood, particularly in low and middle income countries. Genetic improvement of aquaculture species has major untapped potential to help achieve this, with selective breeding and genome editing offering exciting avenues to expedite this process. However, limitations to these breeding and editing approaches include long generation intervals of many fish species, alongside both technical and regulatory barriers to the application of genome editing in commercial production. Surrogate broodstock technology facilitates the production of donor-derived gametes in surrogate parents, and comprises transplantation of germ cells of donors into sterilised recipients. There are many successful examples of intra- and inter-species germ cell transfer and production of viable offspring in finfish, and this leads to new opportunities to address the aforementioned limitations. Firstly, surrogate broodstock technology raises the opportunity to improve genome editing via the use of cultured germ cells, to reduce mosaicism and potentially enable in vivo CRISPR screens in the progeny of surrogate parents. Secondly, the technology has pertinent applications in preservation of aquatic genetic resources, and in facilitating breeding of high-value species which are otherwise difficult to rear in captivity. Thirdly, it holds potential to drastically reduce the effective generation interval in aquaculture breeding programmes, expediting the rate of genetic gain. Finally, it provides new opportunities for dissemination of tailored, potentially genome edited, production animals of high genetic merit for farming. This review focuses on the state-of-the-art of surrogate broodstock technology, and discusses the next steps for its applications in research and production. The integration and synergy of genomics, genome editing, and reproductive technologies have exceptional potential to expedite genetic gain in aquaculture species in the coming decades.

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Suitability of hybrid mackerel (Scomber australasicus × S. japonicus) with germ cell-less sterile gonads as a recipient for transplantation of bluefin tuna germ cells

Cited by 22 publications

References 50 publications

Who is the best surrogate for germ stem cell transplantation in fish?

Who is the best surrogate for germ stem cell transplantation in fish?

Interaction between sex-determining genes from two species: clues fromXenopushybrids

Surrogate broodstock to enhance biotechnology research and applications in aquaculture

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