Sugarcane somatic embryogenesis: a scanning electron microscopy study

Rodríguez, S.; Mondejar, C. Bonmati; Ramos, Martín; Díaz, Efraín Acosta; Maribona, R. H.; Ancheta, Odelsa

doi:10.1016/s0040-8166(96)80003-6

Cited by 12 publications

(5 citation statements)

References 21 publications

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“…Similar morphology has also been found in EC of kiwifruit [ 43 ]. Furthermore, studies in sugarcane [ 44 ] and Citrus hybrid callus [ 45 ] reported that the absence of fibrils on the cell surface observed using SEM, indicated non-embryogenic calli, a result similar to that found in this study for WC in B . rotunda .…”

Section: Resultssupporting

confidence: 87%

Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)

Karim

Tan

et al. 2016

PLoS ONE

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Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together with histological characterization. Metabolite profiling showed relatively higher levels of glutamine, arginine and lysine in embryogenic callus than in dry and watery calli, while shoot base tissue showed an intermediate level of primary metabolites. For the five secondary metabolites analyzed (ie. panduratin, pinocembrin, pinostrobin, cardamonin and alpinetin), shoot base had the highest concentrations, followed by watery, dry and embryogenic calli. Furthermore, intracellular auxin levels were found to decrease from dry to watery calli, followed by shoot base and finally embryogenic calli. Our morphological observations showed the presence of fibrils on the cell surface of embryogenic callus while diphenylboric acid 2-aminoethylester staining indicated the presence of flavonoids in both dry and embryogenic calli. Periodic acid-Schiff staining showed that shoot base and dry and embryogenic calli contained starch reserves while none were found in watery callus. This study identified several primary metabolites that could be used as markers of embryogenic cells in B. rotunda, while secondary metabolite analysis indicated that biosynthesis pathways of these important metabolites may not be active in callus and embryogenic tissue.

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Section: Resultssupporting

confidence: 87%

Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)

Karim

Tan

et al. 2016

PLoS ONE

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“…Regarding the results described in the literature and here observed the morphological variations of callus and shoot regeneration pathways are frequently reported in studies of in vitro shoot induction of sugarcane. The association of organogenic and embryogenic callus on the same sugarcane explant and the plant regeneration through indirect organogenesis from friable callus has been described in some studies (GNANAPRAGASAM & VASIL, 1992;TAYLOR et al, 1992;RODRIGUEZ et al, 1995). In another study, FRANKLIN et al (2006) reported the shoots induction of sugarcane from globular structures associated with the original explant and suggest that somatic embryos in globular stage may lead shoots induction from indirect organogenesis in the presence of cytokines, confirming the results observed here.…”

Section: Histological Analysissupporting

confidence: 89%

Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

et al. 2012

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“…This result was similar with inducing indirect somatic embryogenesis in Arabidopsis using 2,4-D that only needed 10-DAC for callusing (Raghavan 2004) and direct organogenesis in Passiflora that only needed few days (Fernando et al 2007). In sugarcane, callus as first response of explants cultured on induction medium (containing 2,4-D, kinetin, and IAA) could be seen at 3-DAC (Rodríguez et al 1996).…”

supporting

confidence: 70%

“…Surface ultrastructure analysis in maize revealed callus occurrence when shoot tips explant cultured on MS medium containing 2,4-D, benzyladenine, and adenin (Marín-Méndez et al 2009). Rodríguez et al (1996) revealed that sugarcane's callus was also as first response when spindle explants cultured on MS medium containing 2,4-D, kinetin, and IAA using SEM.…”

mentioning

confidence: 98%

Scanning Electron Microscopy Analysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid

Eskundari,

Taryono,

Indradewa

et al. 2024

J.Tropical Biodiversity Biotechnology

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Camellia sinensis L. is an important crop in Indonesia as healthy beverage that contains several secondary metabolism compounds, such as polyphenols and catechins. Tissue culture including somatic embryogenesis and organogenesis has been used for propagating plant for various needs. In this present short-communication, scanning electron microscopic (SEM) analysis of tea was conducted and discussed. This study aimed to investigate surface ultrastructure of TRI2025 embryo axis tea clone cultured on Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D). The results revealed two different forms of explant’s development, i.e. somatic embryo and transitional form between somatic embryogenesis and organogenesis; or called by “Globular-like Structure” (GLS). Surface ultrastructure analysis of somatic embryo and GLS revealed respectively many stages of somatic embryo development i.e. globular, torpedo, and cotyledon stage, and leaf development form GLS regeneration.

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Sugarcane somatic embryogenesis: a scanning electron microscopy study

Cited by 12 publications

References 21 publications

Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)

Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)

Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

Scanning Electron Microscopy Analysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid

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