Sugarcane proteins and messenger RNAs regulated by salt in suspension cells

Abstract

Changes in gene expression induced by salinity were investigated in a suspension culture of the tropieal, graminaceous plant sugarcane {Saeeharum spp,). Three cell lines tolerant to 5, 10 and 15kg m"-' NaCl were adapted and the expression of newly synthesized proteins and translatable mRNAs in these cell lines were compared with those in unadapted cells on highresolution, two-dimensional gels. Specific proteins and mRNAs alteted by salinity were identified. At the protein level, the expression of 15 proteins was induced or enhanced, and that of three others was lepressed or abolished. At the translatable mRNA level, the expression of 18 mRNAs was induced or enhanced, and that of eight others was repressed or abolished. The expression of some of these was also regulated, depending on the salt-adapted cell line. The above changes were characteristic of salt-adapted cells and were not found in unadapted cells undergoing a short-term salt stress. Overall, 29 sugarcane proteins were regulated by salinity: for 15, translatable mRNAs were found; for three, no translatable mRNA was detected; and for six of the remaining 11, in vivo equivalents were not observed. These data suggest that a multitude of mechanistns at the transcriptional, post-transcriptional and post-translational levels tnay contribute to the control of gene expression in the salt-adapted sugarcane cells.

Effect of salt stress on plant gene expression: A review

“…A number of genes are differentially expressed, some in a genotype specific manner (Gulick and Dvo~fik, 1987;Hurkman et al, 1989;Ramagopal, 1987a;Ramagopal and Carr, 1991;Robinson et al, 1990). Although confirming data are required, these results suggest that gene expression is regulated during salt stress by both transcriptional and posttranscriptional mechanisms.…”

“…The identification of genes whose expression enables plants to adapt to or tolerate salt stress is essential for breeding programs, but little is known about the genetic mechanisms for salt tolerance. Recent research demonstrates that salt stress modulates the levels of a number of polypeptides and mRNAs in cultured cells adapted to high levels of salt (citrus: Ben-Hayyim et al, 1989; Distichlis spicata: Zhao et al, 1989;rice: Shirata and Takagishi, 1990; sugarcane: Ramagopal and Carr, 1991;tobacco: Singh et al, 1985;tomato: King et al, 1986), in callus cultures adapted to high levels of salt (alfalfa: Winicov et al, 1989;1990;barley: Ramagopal, 1988b; maize: Ramagopal, 1986), in relatively salt-tolerant glycophytes (barley: Hurkman et al, 1989;Hurkman and Tanaka, 1987;Ramogopal 1987a;1988a;Robinson et al, 1990; wheat amphiploid: Gulick and Dvo~fik, 1987), and in halophytes (Mesembryanthemum crystallinum: Ostrem et al, 1987;Michalowski et al, 1989). Although the detection of gene products that respond specifically to salt stress is a significant finding, they must be identified, functions assigned, and their relation to salt tolerance determined.…”

Sugarcane proteomics: An update on current status, challenges, and future prospects

“…Introduction of 2DE by O'Farrell had rejuvenated and rekindled interest on sugarcane proteomics as evidenced by an influx of research papers during the late 1980s and after by employing 2DE to study sugarcane‐suspension cells , meristem , leaves , stalk , root . Moisyadi and Harrington employed tube gels and 2DE to analyze the effect of heat shock treatments on cultured sugarcane cells.…”

“…proteomics-based studies in sugarcane from 1975 to 1989, probably due to intervention of genomic tools like PCR that has revolutionized genomics and made it seem more attractive and promising than proteomics at that point of time. Introduction of 2DE by O'Farrell [26] had rejuvenated and rekindled interest on sugarcane proteomics as evidenced by an influx of research papers during the late 1980s and after by employing 2DE to study sugarcane-suspension cells [27,28], meristem [29], leaves [30][31][32][33][34][35][36][37][38][39][40], stalk [41], root [42]. Moisyadi and Harrington [27] employed tube gels and 2DE to analyze the effect of heat shock treatments on cultured sugarcane cells.…”

Stress hydrique: étude des mécanismes moléculaires et des modifications de l'expression du génome

“…Les ARNm qui s'accumulent plus tardivement seraient induits par l'ABA (Guy, 1990 ;Ho et Mihkind, 1991). Les polypeptides de Mm similaires à ceux détectés chez la Tomate s'accumulent chez d'autres espèces, en réponse aux stress hydriques (Gomez et al, 1988 ;Vartanian et al, 1987) ou aux stress salins (Mundy et Chua, 1988 ;Ramagopal, 1987 ;Ramagopal et Carr, 1991). A la suite d'un stress hydrique, des protéines riches en glycine s'accumulent dans les feuilles (Gomez e al., 1988 ;Ho et Mishkind, 1991 ;Mundy et Chua, 1988), les racines (Mundy et Chua, 1988) et les embryons (Baker et al, 1988 ;Dure et al, 1989 ;Gomez al., 1988).…”