Sugar occurring in the extrafloral exudates of the orchidaceae

Nectar, nectaries, flower visitors, and breeding system in five terrestrial Orchidaceae from central Argentina

“…50%, wt/ wt). The qualitative (Frey-Wyssling and H&userman 1960, Percival 1961, Baskin and Bliss 1969, Jeffrey et al 1970 and eta/. 1986, Gottsberger et al 1984, Freeman et al 1991 nectar studies performed so far showed that Orchidaceae species have the three most common nectar sugars present in flowering plants.…”

“…Most orchids are self-compatible, but the pollination mechanisms favour outcrossing (van der Pijl andDodson 1966, Dressier 1990) The interesting perspective of the Orchidaceae from the floral biology point of view contrasts with the comparatively small number of investigations carried out in such a big family. Although nectar is considered one of the most important rewards, sugar composition has only been identified in some 110species (Daumann 1941, FreyWyssling and H&userman 1960, Percival 1961, Payne 1965, Baskin and Bliss 1969, Jeffrey et al 1970 and, as far as we know, sugar proportions have been investigated in four species (Pais and Chaves das Neves 1980, Gottsberger et al 1984, Pais et al 1986, Freeman et al 1991. On the other hand, it is assumed the existence of floral nectaries, but little information is available on the structure and location of them (cf.…”

Amino Acid and Sugar Content of the Nectar Exudate From Limodorum Abortivum (Orchidaceae). Comparison With Epipactis Atropurpurea Nectar Composition

“…Floral and extrafloral nectaries from epiphytic and terrestrial orchids have been studied by different authors (M AURIZIO , 1959 ;B ASKIN and BLISS, 1969 ;A RDITTI et al, 1971). Particular attention has been paid to the sugar content.…”

Bees use honest floral signals as indicators of reward when visiting flowers

“…Afterwards, 50 μL of the solution was dried at 60°C and derivatised with 100 μL of a mixture of anhydrous pyridine (Fisher Scientific, Geel, Belgium), hexamethylsilazane (Sigma‐Aldrich, Buchs, Switzerland) and trimethylchlorosilane (Sigma‐Aldrich, Buchs, Switzerland) (10 : 5 : 3) as described in Sweeley et al . () and Baskin & Bliss (). Subsequently, samples were run by GC‐MS as described in Medeiros & Simoneit () (see also Supporting Information methods).…”

“…The sector on the filter paper containing the nectar was cut from the rest of the filter paper and nectar was eluted in 1 mL high-purity Mili-Q water by shaking the dilution for 90 min with 400 rpm at 60°C on a laboratory shaker. Afterwards, 50 lL of the solution was dried at 60°C and derivatised with 100 lL of a mixture of anhydrous pyridine (Fisher Scientific, Geel, Belgium), hexamethylsilazane (Sigma-Aldrich, Buchs, Switzerland) and trimethylchlorosilane (Sigma-Aldrich, Buchs, Switzerland) (10 : 5 : 3) as described in Sweeley et al (1963) and Baskin & Bliss (1969). Subsequently, samples were run by GC-MS as described in Medeiros & Simoneit (2007) (see also Supporting Information methods).…”