Suction blister epidermal grafting for vitiligo involving angles of lip: Experience of 112 patients

Kar, Bikash Ranjan; Raj, Chinmoy

doi:10.4103/jcas.jcas_111_15

Cited by 16 publications

(15 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One advantage is the rapid re‐epithelization of the donor site without visible scarring (Figure ). With vitiligo, SBEG can even be used in challenging areas such as the face, including the eyelids, the perioral area and the lips . There are no reported contraindications and the side effects are insignificant, providing a good option even for multimorbid patients who may be poor candidates for surgery.…”

Section: Resultsmentioning

confidence: 99%

Suction blister epidermal graft (SBEG) – an easy way to apply this method

Angeletti

Kaufmann

2019

J Deutsche Derma Gesell

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Suction blister epidermal graft (SBEG) – an easy way to apply this method

Angeletti

Kaufmann

2019

J Deutsche Derma Gesell

View full text Add to dashboard Cite

“…Many suction blister studies employ mono-chamber devices, often utilizing syringes with the plungers removed and attached to vacuum pumps [ 13 , 14 ]. These chambers can be cumbersome, cannot assure equal pressure between independent chambers, and/or lack the ability to monitor the pressure during the application.…”

Section: Resultsmentioning

confidence: 99%

Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632

et al. 2018

View full text Add to dashboard Cite

Keratinocytes are the most abundant cell type in the epidermis. They prevent desiccation and provide immunological and barrier defense against potential pathogens such as Staphylococcus aureus and Candida albicans. The study of this first line of immune defense may be hindered by invasive isolation methods and/or improper culture conditions to support stem cell maintenance and other potential mechanisms contributing to long-term subcultivation in vitro. Primary keratinocytes have been successfully isolated from blister roofs induced by negative pressure, which separates the epidermis from the dermis in vivo in human subjects. This method allows collection of pure epidermal cells without dermal contamination in a minimally invasive manner. However, the isolated keratinocytes differentiate and senesce when cultured in vitro beyond five passages. Here, we present evidence that the Rho kinase (ROCK) inhibitor Y-27632 can be used to effectively increase the proliferative capabilities of keratinocytes isolated using the suction blister method, similar to what has been previously reported for primary keratinocytes isolated using alternative methods. We show that the increase in passage number is directly correlated to delayed differentiation, and that cells passaged long term with the inhibitor retain their ability to stratify in organotypic raft cultures and respond to cytokine treatment; additionally, the late passage cells have a heterogeneous mix of differentiated and non-differentiated cells which may be predicted by a ratio of select differentiation markers. The described method presents a minimally invasive procedure for keratinocyte isolation and prolonged culture that allows analysis of keratinocyte function in both healthy volunteers and patients with dermatologic diseases.

show abstract

“…Many suction blister studies employ mono-chamber devices, often utilizing syringes with the plungers removed and attached to vacuum pumps [12, 13]. These chambers can be cumbersome, cannot assure equal pressure between independent chambers, and/or lack the ability to monitor the pressure during the application.…”

Section: Resultsmentioning

confidence: 99%

Prolonged culture of primary human keratinocytes isolated from suction blisters

Anderson

Sastalla

Earland

et al. 2018

Preprint

View full text Add to dashboard Cite

Keratinocytes are the most abundant cell type in the epidermis. They prevent desiccation and provide immunological and barrier defense against potential pathogens such as Staphylococcus aureus and Candida albicans. The study of this first line of immune defense is hindered by invasive isolation methods and insufficient techniques for long-term passage of primary keratinocytes in vitro. Primary keratinocytes have been successfully isolated from blister roofs induced by negative pressure, which separates the epidermis from the dermis in vivo in human subjects. This method allows collection of pure epidermal cells without dermal contamination in a minimally invasive manner. However, the isolated keratinocytes differentiate and senesce when cultured in vitro beyond five passages. Here, we present evidence that the Rho kinase (ROCK) inhibitor Y-27632 can be used to effectively increase the proliferative capabilities of keratinocytes isolated using the suction blister method, similar to what has been previously reported for primary keratinocytes isolated using alternative methods. We show that the increase in passage number is directly correlated to delayed differentiation, and that cells passaged long term with the inhibitor retain their ability to stratify in organotypic raft cultures and respond to cytokine treatment; additionally, the late passage cells have a heterogeneous mix of differentiated and non-differentiated cells which may be predicted by a ratio of select differentiation markers. The described method presents a minimally invasive procedure for keratinocyte isolation and prolonged culture that allows analysis of keratinocyte function in both healthy volunteers and patients with dermatologic diseases.

show abstract

Suction blister epidermal grafting for vitiligo involving angles of lip: Experience of 112 patients

Cited by 16 publications

References 26 publications

Suction blister epidermal graft (SBEG) – an easy way to apply this method

Suction blister epidermal graft (SBEG) – an easy way to apply this method

Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632

Prolonged culture of primary human keratinocytes isolated from suction blisters

Contact Info

Product

Resources

About