Sucrose Synthase, Starch Accumulation, and Tomato Fruit Sink Strength

Wang, Fei; Sanz, Ana Gimeno; Brenner, Mark L.; Smith, Alan G.

doi:10.1104/pp.101.1.321

Cited by 270 publications

(188 citation statements)

References 16 publications

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“…The "t" stands for the number of days after pollination. Sink activity was expressed as the increase in dry weight per unit of dry weight present per unit of time using the equation (DW t2 ¡ DW t1 )/(t2 ¡ t1) as described by Wang et al (1993).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Differences in regulation of carbohydrate metabolism during early fruit development between domesticated tomato and two wild relatives

Kortstee

Appeldoorn

Oortwijn

et al. 2007

Planta

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Early development and growth of fruit in the domesticated tomato Solanum lycopersicum cultivar Money Maker and two of its wild relatives, S. peruvianum LA0385 and S. habrochaites LA1777, were studied. Although small diVerences exist, the processes involved and the sequence of events in fruit development are similar in all three species. The growth of developing fruits is exponential and the relative growth rate accelerates from 5 days after pollination (DAP 5) to DAP 8, followed by a decline during further development. Growth is positively correlated to the standard "Brix plus starch'' in the period DAP 8-DAP 20. Carbohydrate composition and levels of sugars and organic acids diVer in fruits of the wild accessions compared to domesticated tomato. The wild accessions accumulate sucrose instead of glucose and fructose, and ripe fruits contain higher levels of malate and citrate. The enzymes responsible for the accumulation of glucose and fructose in domesticated tomatoes are soluble invertase and sucrose synthase. The regulation of initial carbohydrate metabolism in the domesticated tomato diVers from that in the wild species, as could be concluded from measuring activities of enzymes involved in primary carbohydrate metabolism. Furthermore, changes in the activity of several enzymes, e.g., cell wall invertase, soluble invertase, fructokinase and phosphoglucomutase, could be attributed to changes in gene expression level. For other enzymes, additional control mechanisms play a role in the developing tomato fruits. Localization by in-situ activity staining of enzymes showed comparable results for fruits of domesticated tomato and the wild accessions. However, in the pericarp of S. peruvianum, less activity staining of phosphogluco-isomerase, phosphoglucomutase and UDPglucosepyrophosphorylase was observed.

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Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Differences in regulation of carbohydrate metabolism during early fruit development between domesticated tomato and two wild relatives

Kortstee

Appeldoorn

Oortwijn

et al. 2007

Planta

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“…A positive correlation between sucrose synthase activity, sink strength and sucrose import has been demonstrated in many sink organs, suggesting a role for this enzyme in carbohydrate partitioning between sink and source organs [24,28,30]. In addition, SuSy appears to play a key role in supplying energy for loading and unloading in phloem by providing substrate for respiration [16].…”

Section: Introductionmentioning

confidence: 99%

“…SuSy is ubiquitous in higher plants and assigns a variety of important roles. It is the predominant sucrose cleavage enzyme in cereal endosperm and in storage organs such as tubers, fruits, seed or immature leaves [24,28]. Sucrose synthase in these carbohydrate sink organs provides substrates for respiration [29], 586 R. Le Hir et al…”

Section: Introductionmentioning

confidence: 99%

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Hir¹,

Pelleschi-Travier²,

Viémont³

et al. 2005

Ann. For. Sci.

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-In the search for a trophic control of rhythmic growth of young oak seedlings, sucrose synthase accumulation, localization and activity were studied in the apex, underlying internodes and leaves, during the second flush of shoot growth. The use of an anti-SuSy antibody raised against the Vicia faba protein allowed the detection of SuSy, which showed a 90 kDa subunit. This antibody, used to immunolocalize the SuSy protein in oak revealed a positive signal in the reserve parenchyma tissues of the apex and in the leaf vascular tissues and underlying internodes. The study of SuSy activity along with the growth pattern of the different organs, suggested that SuSy may be involved in the control of rhythmic growth through the mobilization of sucrose in storage tissues and through loading/unloading processes. Such activities would help to bring enough nutrients to support the high morphogenic and growth processes during the rhythmic growth of the shoot.common oak / rhythmic growth / bud / sugar metabolism / sink strength Résumé -Localisation et activité de la saccharose synthase au cours de la croissance rythmique de l'axe aérien du chêne pédonculé (Quercus robur L.). Afin d'évaluer l'existence d'un contrôle trophique dans la croissance rythmique, l'accumulation, la localisation et l'activité de la saccharose synthase ont été étudiées au sein de l'apex, de l'entre-noeud sous-jacent et des feuilles au cours de la deuxième vague de croissance chez le chêne pédonculé. L'utilisation d'un anticorps anti-SuSy de Vicia faba a permis de détecter la protéine SuSy chez le chêne, qui présente une sous-unité de 90 kDa. Cet anticorps a révélé un signal positif dans les parenchymes de réserves de l'apex et dans les tissus vasculaires des feuilles et des entre-noeuds sous-jacents. L'étude de l'activité SuSy au cours de la croissance des différents territoires suggère que cette enzyme pourrait être impliquée dans le contrôle de la croissance rythmique au travers de la mobilisation des réserves glucidiques et des processus de chargement/déchargement phloémien. Ces activités apporteraient l'énergie nécessaire aux processus de morphogenèse et de croissance observés durant la croissance rythmique de la tige. chêne pédonculé / croissance rythmique / bourgeon / métabolisme glucidique / force de puits

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“…The sink strength can be described as the product of sink size and sink activity (Venkateswarlu and Visperas 1987). Sink activity is a physiological restraint that includes multiple factors and key enzymes (invertase and sucrose synthase) involved in carbohydrate use and storage (Wang et al 1993). Activity of acid and alkaline invertase investigated in the present study in the accessions of T. dicoccoides and cultivated wheats demonstrated significant genotypic variation and far higher activity of enzymes in T. dicoccoides relative to cultivated wheats.…”

Section: Discussionmentioning

confidence: 59%

Differential response of wild and cultivated wheats to water deficits during grain development: changes in soluble carbohydrates and invertases

Suneja

Gupta

Sharma

et al. 2015

Physiol Mol Biol Plants

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Wheat, staple food crop of the world, is sensitive to drought, especially during the grain-filling period. Water soluble carbohydrates (WSCs), stem reserve mobilization and higher invertase activity in the developing grains are important biochemical traits for breeding wheat to enhance tolerance to terminal drought. These traits were studied for three accessions of Triticum dicoccoides(a tetraploid wheat progenitor species) -acc 7054 (EC 171812), acc 7079 (EC 171837) and acc 14004 (G-194-3 M-6 M) selected previously on the basis of grain filling characteristics. Check wheat cultivars-PBW-343 (a popular bread wheat cultivar for irrigated environments) and C-306 (widely adapted variety for rain-fed agriculture) were also included in this set. Analysis of variance revealed significant genotypic differences for the content of water soluble carbohydrates, activity of acid invertase and alkaline invertase. Acc 7079 was found to be a very efficient mobilizer of water soluble carbohydrates (236.43 mg g -1 peduncle DW) when averaged over irrigated and rain-fed conditions. Acid invertase activity revealed marked genotypic differences between wild and cultivated wheats. Alkaline invertase activity was highest in Acc 7079 when pooled across both the environments. On the whole, acc 7079 qualifies as a suitable donor for enhancing tolerance of bread wheat to terminal drought. The association of physiobiochemical differences observed with grain filling attributes on one hand and molecular markers on the other could be of use in improving wheat for water stress conditions.

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Sucrose Synthase, Starch Accumulation, and Tomato Fruit Sink Strength

Cited by 270 publications

References 16 publications

Differences in regulation of carbohydrate metabolism during early fruit development between domesticated tomato and two wild relatives

Differences in regulation of carbohydrate metabolism during early fruit development between domesticated tomato and two wild relatives

Sucrose synthase expression pattern in the rhythmically growing shoot of common oak (Quercus robur L.)

Differential response of wild and cultivated wheats to water deficits during grain development: changes in soluble carbohydrates and invertases

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