SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE2.6, an Ortholog of OPEN STOMATA1, Is a Negative Regulator of Strawberry Fruit Development and Ripening

Identification and Transcript Analysis of the TCP Transcription Factors in the Diploid Woodland Strawberry Fragaria vesca

“…The strawberry 26S-18S RNA gene (housekeeping gene) was used as an internal control to normalize the expression data. The ripening-related gene primers are listed in Supplementary Table S3 (Han et al, 2015). Asterisks indicate significant differences compared with the control sample, as determined by the Student’s t -test ( ∗ p < 0.05, ∗∗ p < 0.01).…”

“…C15 empty vector and Agrobacterium tumefaciens strain GV3101 served as the control. Agrobacterium -mediated transient assays were performed as described previously with some modifications (Han et al, 2015). The Agrobacterium suspension was evenly injected throughout the entire Fragaria × ananassa Duch.…”

“…For each construct, five uniformly sized fruits were used in the infiltration experiment, and the experiment was repeated three times. The primers used for RT-qPCR analysis of ripening-related genes are listed in Supplemental Table S3 (Han et al, 2015). …”

The Jasmonate-Activated Transcription Factor MdMYC2 Regulates ETHYLENE RESPONSE FACTOR and Ethylene Biosynthetic Genes to Promote Ethylene Biosynthesis during Apple Fruit Ripening

“…Due to the long juvenile period of transgenic apple plants, we used a transient expression assay to silence MdMYC2 expression in apple fruit by Agrobacterium infiltration, a widely used method for studying gene function (Jia et al, 2011;Wang et al, 2013;Han et al, 2015;Li et al, 2016). MdMYC2-suppressed apple fruit (MdMYC2-AN) showed slower ripening than control fruits after MeJA treatment ( Figure 9C), as well as significantly lower MdACS1 and MdACO1 expression ( Figures 9D and 9E) and ethylene production ( Figure 9G).…”

Sigma factor FaSigE positively regulates strawberry fruit ripening by ABA

“…Our data suggest that SigE, FaABAR, sugars, and ABA are associated with a complex regulatory signaling network affecting strawberry fruit ripening. Given important roles of ABA and sugar in ripening (Han et al 2015;Wang et al 2015;Razzaq et al 2016;Zhao et al 2017), future studies should investigate SigE-related two-component systems and characterize the molecular mechanism that enables SigE to regulate strawberry fruit ripening via ABA signaling pathway.…”