Sigma factor FaSigE positively regulates strawberry fruit ripening by ABA

A leu-rich repeat receptor-like protein kinase, FaRIPK1, interacts with the ABA receptor, FaABAR, to regulate fruit ripening in strawberry

“…It is thought that ABA perception sites may be tissue-, organ-, or even cell-specific, as well as specific to the developmental stage ( Wang and Zhang, 2008 ). Strawberry FaABAR is an ABA receptor that serves an inducer of fruit ripening ( Jia et al , 2011 a ; Zhang et al , 2017 ), and identification of FaABAR-interacting proteins can therefore provide insights into the action of ABA in ripening. We used strawberry fruit at the white stage, which is a distinct stage related to ripening ( Wang et al , 2017 ), to generate a high-quality cDNA library that could be used as a basis for subsequent Y2H screening.…”

“…Using Tobacco rattle virus-induced gene silencing (VIGS) in strawberry fruit, down-regulation of FaABAR expression was shown to inhibit ripening, and the white phenotype of these RNAi fruits could not be rescued by addition of exogenous ABA, demonstrating that ABAR positively regulates ripening under normal conditions ( Jia et al , 2011 a ). Importantly, Kadomura-Ishikawa et al (2015) found that FaMYB10 plays a role as a signal transduction mediator from ABAR perception of ABA to anthocyanin synthesis during strawberry fruit ripening, and it was recently reported that FaABAR can bind to ABA ( Zhang et al , 2017 ).…”

Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening

“…The ABA receptors PYR1 and ABAR have recently been characterized in grape [93,94] and strawberry [19,48,95,96]. In 'Kyoho' grape, the expression level of PYR1-like gene (VlPYL1) was highest in fruit and also in leaf tissue, with an increase of this transcript during fruit development, and a consequent reduction in ripe berries [93].…”

“…In strawberry, transcriptional analysis in achenes and receptacle during ripening was the first antecedent for the role of ABA/PYR pathway, observing differential expression of genes encoding for protein phosphatase 2C (PP2C), RAS-related small GTP binding protein, and putative serine/threonine protein kinase (ARSK1) [97]. Later, it was demonstrated that under ABA stimulation the ABAR promotes ripening in strawberry fruit [19,48,95], through ABA binding [96]. The downregulation of FaABAR expression inhibits ripening, without rescued by addition of exogenous ABA, suggesting a crucial role for the ABAR receptor in strawberry ripening [46].…”

Polyamines Regulate Strawberry Fruit Ripening by Abscisic Acid, Auxin, and Ethylene

“…Based on previous reports (Jia et al, 2013;Zhao et al, 2017;Zhang et al, 2017) and our RNA-seq data, the expression levels of various genes and their corresponding physiological parameters, including firmness (PG1 and PL1), anthocyanin content (CHS and DFR), sugar content (SUT1 and SS), polyamine content (ADC, ODC, SPMS, and SPDM), IAA levels and signaling (IAA2, ARF11, and LUX2), ABA levels and signaling (ABI37, SnRK2, and NCED1), and ethylene emissions and signaling (ACO1 and EBF1), were analyzed in the RNAi and OE fruits, in which FaSAMDC expression levels were down-and up-regulated over 80% compared with the mixed control fruits. The results showed that in comparison to the control, both Spm and Spd contents were higher in OE fruits and lower in the RNAi fruits, but Put contents were lower in the OE fruits and higher in the RNAi fruits ( Fig.…”