Sucrose hydrolysis by invertase using a membrane reactor: effect of membrane cut-off on enzyme performance

Abstract

Sucrose hydrolysis by invertase [EC.3.2.1.26] produces inverted sugar syrup, an ingredient mainly used in the food industry. To properly catalyze hydrolysis, the enzyme should be reused after this reaction. It is advisable to maintain constant activity over a considerable period. Thus, sucrose hydrolysis was performed in a membrane bioreactor -a continuously stirred tank reactor coupled with an ultrafiltration membrane (UFM) which provides good diffusion and high activity per unit volume. Molecular weight cut-off for soluble invertase UFMs was up to 100kDa. This study focused on the role of UFM invertase cut-off as it is the main element in the process. We demonstrated that both the cut-off and chemical nature of the UFM affected specific invertase activity. Uniterms:Invertase. Membrane Reactor.Sucrose/hydrolysis.A hidrólise da sacarose através da invertase [EC.3.2.1.26] gera xarope de açúcar invertido, que é usado principalmente como ingrediente na indústria alimentícia. Para ter-se uma hidrólise satisfatória, a enzima deve ser reaproveitada após a reação. É desejável que sua atividade seja mantida constante durante um período considerável de reação. A hidrólise da sacarose foi realizada em um reator com membrana (RM) -que é um reator continuamente agitado acoplado a uma membrana de ultrafiltração (MUF) -, porque apresenta efeitos mínimos de difusão e elevada atividade por unidade de volume. O corte molecular da MUF utilizada para reter a invertase solúvel dentro do MR foi de até 100kDa. Como a invertase é o elemento principal deste processo, este trabalho foi focado no papel do corte molecular da MUF na sua atividade. O corte molecular e a natureza química da membrana-UF mostraram afetar a atividade específica da invertase.Unitermos: Invertase. Reator de Membrana. Sacarose/hidrólise.

Sakkarozun Enzimatik Olarak Hidrolizinin Optimizasyonu

“…Kullanılan asit çevreyi kirletmesinin yanında ekipman için korozif etkiye sahip olması ve yan ürünlerin oluşması gibi dezavantajlara sahiptir. Yüksek özgüllük ve afiniteye sahip enzimatik işlem çok daha ılımlı şartlarda gerçekleşen bir reaksiyondur ve yan ürün oluşumunun olmaması enzimatik yöntemin önemli avantajlarıdır [35].…”

Study the Behavior of Activity and Express for Enzymes

“…For example, the hydrolysis of sucrose by invertase is expressed as total-reducing sugars formed per minute, the collagen hydrolysis by collagenase is expressed as the viscosity diminution of a standard collagen solution per minute, and the amylase activity expressed as a SKB unit (10),(11), (12) . However, it is recommended to express the enzyme activity using International Unit (U), which is defined as the amount of enzyme that catalyzes the formation of 1 mole of (13,14) product per minute in fixed conditions. The specific activity is defined as U/mg protein (if the enzyme preparation is impure), or U/mol enzyme (for pure enzymes) (15) .…”

Structural Properties, Production, and Commercialisation of Invertase

“…In the food and beverage industries, invertase has been largely employed in the production of invert syrup and high fructose syrup (Addezio et al 2014;Ashraf & Bilal 2015). Invertase is used as an alternative to the traditional acid hydrolysis method which might produce undesirable by-products, unfavourable colour, and bitter taste; could also result in low conversion efficiencies and high ash contents (Aburigal et al 2014).…”